Histopathological and HPLC analysis in the hepatic tissue of Pangasius sp. exposed to diclofenac

• Autorzy: Geetha V. , Sujata R. , Shreenidhi K.S. , Sundararaman T.R.
• Języki publikacji: EN

Abstrakty

EN

Pharmaceutics present in the environment are of growing concern for their potential consequences on human and ecosystem health. In long-term monitoring investigations of sewage and surface water samples, diclofenac was identified as one of the most important pharmaceutically active compounds. Pangasius sp. were exposed to determine the lethal concentration (LC₅₀) of diclofenac. The histopathological studies in a fish hepatopancreas sample showed significant changes like nucleation, differentiation, and hepatocytes. The large lesions and infiltration in hepatocytes were distinctly seen due to the toxic effect of diclofenac. In HPLC analysis, the control fish liver tissue showed no significant changes, whereas the diclofenac-exposed fish liver tissue showed a significant peak with a quicker run time. The studies confirmed that the diclofenac compound affects hepatocytes to a noteworthy extent. Therefore, this calls for reduction of diclofenac usage and also to have a checkpoint especially for diclofenac in the pharmaceutical effluent released into the water bodies.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2018
• Tom: 27
• Numer: 6
• Opis fizyczny: p.2493-2498,fig.,ref.

Twórcy

autor

Geetha V.

• Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, Chennai, India

autor

Sujata R.

• Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, Chennai, India

autor

Shreenidhi K.S.

• Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, Chennai, India

autor

Sundararaman T.R.

• Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, Chennai, India

Bibliografia

• 1. PADMINI E., VIJAYAGEETHA B. Biodegradative Efficiency of Recombinant Escherichia Coli on Heavy Metal Contaminants and Organic Pollutants from Ennore Estuary. Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper. 10 (1), 185, 2008.
• 2. CHANTI BABU PATNEEDI, DURGA PRASADU Impact of Pharmaceutical Wastes On Human Life And Environment, Rasayan J. Chem, 8 (1), 67, 2015.
• 3. KATHRYN E. ARNOLD A. ROSS BROWN GERALD, T. ANKLEY JOHN, SUMPTER P. Medicating the environment: assessing risks of pharmaceuticals to wildlife and ecosystems. Philosophical transactions of the Royal Society B, 369 (1656), 2014.
• 4. GAW S., THOMAS K.V., HUTCHINSON T.H. Sources, impacts and trends of pharmaceuticals in the marine and coastal environment, Philosophical transactions of the Royal Society B, 2014.
• 5. MCGUIRE JOHN L., HASSKARL HORST BODE, GERD KLINGMANN, INGRID ZAHN, MANUEL, Pharmaceuticals-General Survey. Ullmann’s Encyclopedia of industrial chemistry, 2007.
• 6. HALLARE A.V., KOHLER H. R.,TRIEBSKORN R, Developmental toxicity and stress protein responses in zebrafish embryos after exposure to diclofenac and its solvent, DMSO. Chemosphere. 56 (7), 545, 2004.
• 7. VAN DEN BRANDHOF AND MONTFORTS M., Fish embryo toxicity of carbamazepine, diclofenac and metoprolol. Ecotoxicology and environmental safety. 73 (8), 1862, 2010.
• 8. PRASKOVA E., VOSLAROVA E., SIROKA Assessment of diclofenac LC₅₀ reference values in juvenile and embryonic stages of the zebrafish. Polish journal of veterinary sciences, 14 (4), 545, 2011.
• 9. HEBERER T., Occurrence, fate, and removal of pharmaceutical residues in the aquatic environment: a review of recent research data. Toxicology Letters. 131 (1-2), 5, 2002.
• 10. EVA PRASKOVA, LUCIE PLHALOVA, LUCIE CHROMCOVA, STANISLAVA STEPANOVA, IVETA BEDANOVA, JANA BLAHOVA, MARTIN HOSTOVSKY,MISASKORIC, PETR MARŠÁLEK, EVA VOSLAROVA, AND ZDENKA SVOBODOVA., Effects of Subchronic Exposure of Diclofenac on Growth, Histopathological Changes, and Oxidative Stress in Zebrafish (Danio rerio). The Scientific World Journal. 2014, 2014.
• 11. BUSER H.R., POIGER T., MULLER M.D., Occurrence and fate of the pharmaceutical drug diclofenac in surface waters: rapid photodegradation in a lake, Environmental Science and Technology, 32 (22), 3449, 1998.
• 12. SAUVAGE, Pangasius hypophthalmusas seen by World Register of Marine Species, 1878.
• 13. YENGKOKPAM S., SAHU N.P., PAL A.K., MUKHERJEE S.C., DEBNATH D., Haematological and hepatic changes in Catla catla fingerlings in relation to dietary sources and levels of gelatinized carbohydrate.Acta Ichthyol. Piscat. 35 (2), 87, 2005.
• 14. DUBOIS, FLUCHARD, SIOR, DELAHAUT, Identification and quantification of five macrolide antibiotics in several tissues, eggs and milk by liquid chromatography-electrospray tandem mass spectrometry, J Chromatogr B Biomed Sci Appl., 5, 189, 2001.
• 15. CHEN J.B., GAO H.W., ZHANG Y.L., ZHANG Y., ZHOU X.F., LI C.Q., GAO H.P. Developmental toxicity of diclofenac and elucidation of gene regulation in zebrafish (Daniorerio). Sci. Rep., 4 (4841), 1, 2014.
• 16. POPOVIC M., ZAJA R., FENT K., SMITAL T., Interaction of environmental contaminants with zebrafish organic anion transporting polypeptide, Oatp1d1 (Slco1d1). Toxicol Appl Pharmacol., 1; 280 (1), 149, 2014.
• 17. WANG Y., HUANG H., WU Q. Characterization of the zebrafish Ugt repertoire reveals a new class of drug-metabolizing UDP glucuronosyltransferases. Mol Pharmacol. 86 (1), 62, 2014.
• 18. REZA SEYRAFI, GHOLAMREZA NAJAFI, HOOMAN RAHMATI-HOLASCO, Histological study of Hepatopancreas in Iridescent Shark Catfish (Pangasius hypopthalmus). Journal of Animal and Veterinary Advances. 8 (7), 1305, 2009.
• 19. JUSTINA I.R., Histopathological changes in liver and muscle of Tilapia fish from QIRE exposed to concentrations of heavy metals., International journal of biological, biomolecular, agricultural, food and biotechnological engineering, 9 (6), 637, 2015.
• 20. HELAN CHANDRA, SAJDA S. SRIDHAR Acute toxicity studies on the fresh water fish Clarias batrachus exposed to pesticide rogorin., Journal of chemical and pharmaceutical sciences. 8 (1), 34, 2015.
• 21. ISLAS FLORES, LEOBARDO MANUEL GÓMEZ-OLIVÁN, MARCELA GALAR-MARTÍNEZ, ARTURO COLÍN-CRUZ, NADIA NERI-CRUZ, SANDRA GARCÍA-MEDINA Diclofenac-Induced Oxidative Stress in Brain, Liver, Gill and Blood of Common Carp (Cyprinus carpio). Ecotoxicology and Environmental Safety. 92, 32, 2013.
• 22. GHELFI A., RIBAS J.L.C., GUILOSKI I.C., BETTIM F.L., PIANCINI L.D.S., CESTARI M.M., PEREIRA A.J., SASSAKI G.L., SILVA DE ASSIS H.C. Evaluation of Biochemical, Genetic and Hematological Biomarkers in a Commercial Catfish Rhamdia quelen Exposed to Diclofenac, Bulletin of Environmental Contamination and Toxicology, 96 (1), 49, 2016.
• 23. SARAVANAN M., KARTHIKA S., MALARVIZHI A., RAMESH M. Ecotoxicological Impacts of Clofibric Acid and Diclofenac in Common Carp (Cyprinus Carpio) Fingerlings: Hematological, biochemical, Ionoregulatory and Enzymological Responses. Journal of Hazardous Materials. 195, 188, 2011.
• 24. ZI J., LIU D., MA P., HUANG H., ZHU J., WEI D., YANG J., CHEN C., Effects of CYP2C9*3 and CYP2C9*13 on diclofenac metabolism and inhibition-based drug-drug interactions. Drug Metab Pharmacokinet. 25, 343, 2010.

Identyfikatory

DOI

10.15244/pjoes/75829