Effects of lead chloride on human erythrocyte membranes and on kinetic anion sulphate and glutathione concentrations

• Autorzy: Gugliotta T. , De Luca G. , Romano P. , Rigano C. , Scuteri A. , Romano L.
• Języki publikacji: EN

Abstrakty

EN

Our study concerns the effects of exposure to lead chloride on the morphology, K+ efflux, SO4 − influx and GSH levels of the human erythrocyte. Blood was collected in heparinized tubes and washed three times. The cells were suspended at 3% hematocrit and incubated for 1 h at 25°C in a medium containing increasing concentrations of lead chloride (0, 0.3, 0.5 and 1 μM). After incubation, the suspensions were centrifuged and the erythrocyte pellets were divided into three aliquots for testing. The results show: an increase in the permeability of erythrocytes treated with lead chloride with consequent damage and cellular death, especially in the presence of high concentrations; an increase in potassium ion efflux; alterations in the morphology and membrane structure of the red blood cells; and a decrease in sulphate uptake, due either to the oxidative effect of this compound on the band 3 protein, which loses its biological valence as a carrier of sulphate ions, or to a decrease in the ATP erythrocyte concentration. In conclusion, the exposure of erythrocytes to Pb2+ ions leads to a reduction in the average lifetime of the erythrocytes and the subsequent development of anemia. These data are discussed in terms of the possible effect of lead on the reduction-oxidation systems of the cell. Oxidant agents, such as lead, are known to cross-link integral membrane proteins, leading to K/Cl-cotransport. The increased K+ efflux affects the altered redox state.

Słowa kluczowe

EN

lead chloride; human erythrocyte; erythrocyte membrane; kinetic anion sulphate; glutathione concentration; anion transport; scanning electron microscopy; anaemia; adenosine 5'-triphosphate; sulphate

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2012
• Tom: 17
• Numer: 4
• Opis fizyczny: p.586-597,fig.,ref.

Twórcy

autor

Gugliotta T.

• Dipartimento di Scienze della Vita "M.

autor

De Luca G.

autor

Romano P.

autor

Rigano C.

autor

Scuteri A.

autor

Romano L.

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