Assessment of erythroblastic cells in the bone marrow of healthy Holstein-Friesian cows

• Autorzy: Snarska A. , Krystkiewicz W. , Sobiech P. , Zarczynska K. , Rekawek W. , Stopyra A.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to determine the activity and effectiveness of bone marrow hematopoiesis in healthy, adult cattle, and to compare hematopoietic activity levels in diseased cattle. The experimental material comprised 10 high-yielding, clinically healthy Holstein-Friesian cows aged 2-3 years. Bone marrow aspirate smears were analyzed. Marrow samples were collected with a marrow biopsy needle with a length of 63 mm (13G, from the medullary cavity of the third and fourth rib in the sternal region, and the material was subjected to a smear test. Marrow and peripheral blood smears were stained by a method proposed by May Grünwald-Giemsa (MGG). An increase in the number of orthochromatic erythroblasts with a simultaneous decrease in basophilic and polychromatic erythroblast counts was noted in this study. The results indicate that the nucleoli of bovine proerythroblasts are less visible, and the cytoplasm is stained light blue.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2013
• Tom: 69
• Numer: 09
• Opis fizyczny: p.540-542,ref.

Twórcy

autor

Snarska A.

• Department of Internal Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-957 Olsztyn, Poland

autor

Krystkiewicz W.

• Department of Internal Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-957 Olsztyn, Poland

autor

Sobiech P.

• Department of Internal Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-957 Olsztyn, Poland

autor

Zarczynska K.

• Department of Internal Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-957 Olsztyn, Poland

autor

Rekawek W.

• Department of Internal Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-957 Olsztyn, Poland

autor

Stopyra A.

• Department of Internal Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-957 Olsztyn, Poland

Bibliografia

• 1.Ammann V. J., Fecteau G., Helie P., Desnoyers M., Hebert P., Babkine M.: Pancytopenia associated with bone marrow aplasia in a Holstein heifer. Can. Vet. J. 1996, 37, 493-495.
• 2.Ban A., Ogata Y., Kato T.: Erythrocyte morphology and the frequency of spherocytes in hereditary erythrocyte membrane protein disorder in Japanese Black cattle. Bull. Nippon Vet. Anim. Sci. Univ. 1995, 44, 21-27.
• 3.Calhoun M. L.: A cytological study of costal marrow. Am. J. Vet. Res. 1954, 15, 181-196.
• 4.Dirksen G., Gründer H. D., Stöber M.: Choroby wewnętrzne i chirurgia bydła. Galaktyka, Łódź 2007, p. 251.
• 5.Harvey J. W.: Atlas of veterinary hematology. Elsewier, Philadephia 2001, 93.
• 6.Harvey J. W.: Canine bone marrow: normal hematopoiesis, biopsy techniques, and cell identification and evaluation. Comp. Cont. Ed. Pract. Vet. 1984, 6, 909-926.
• 7.Jain N. C.: Essentials of Veterinary Hematology. Lea&Febiger, Philadelphia 1993, 35-67.
• 8.Jain N. C.: Hematologic Techniques, [in:] Schalm's Veterinary Hematology. 4th ed., Lea& Febiger, Philadelphia 1986, 20-86.
• 9.Lawrence W. C., Nichols W. W., Altera K. P.: A simple method for bone marrow aspiration in the cow. Cornell Vet. 1962, 52, 297-305.
• 10.McGillivray S. R., Searcy G. P., Hirsch V. M.: Serum iron, total iron binding capacity, plasma copper and hemoglobin types in anemic and poikilocytic calves. Can. J. Comp. Med. 1985, 49, 286-290.
• 11.Merris V. Van, Meyer E., Dosogne H., Burvenich C.: Separation of bovine bone marrow into maturation-related cell fractions. Vet. Immunol. Immunopathol. 2001, 83, 11-17.
• 12.Merris V. Van, Meyer E., Duchateau L., Burvenrich C.: Differential effects of steroid and retinoid on bovine myelopoiesis in vitro. J. Dairy Sci. 2004, 87, 1188-1195.
• 13.Merris V. Van, Meyer E., Gasthuys F., Burvenich C.: Sternal aspiration of bone marrow in adult cows. Vlaams Diergeneesk. Tijdschr. 2001, 70, 304-306.
• 14.Mischke R.: Praktyczna hematologia psów i kotów. Galaktyka, Łódź 2003, p. 3.
• 15.Morin D. E., Garry F. B., Weiser M. G.: Hematologic responses in llamas with experimentally-induced iron deficiency anemia. Vet. Clin. Pathol. 1993, 22, 81-85.
• 16.Okabe J., Tajima S., Jamato O., Inaba M., Hagiwara S., Maede Y.: Hemoglobin types, erythrocyte membrane skeleton and plasma iron concentration in calves with poikilocytosis. J. Vet. Med. Sci. 1996, 58, 629-634.
• 17.Sato T., Mizuno M.: Poikilocytosis of newborn calves. Nippon. Juigaku. Zasshi. 1982, 44, 801-805.
• 18.Schalm O. W., Lasmanis J.: Cytologic features of bone marrow in normal and mastitic cows. Am. J. Vet. Res. 1976, 37, 359-363.
• 19.Schalm O. W., Lasmanis J., Jain N. C.: Conversion of chronic staphylococcal mastitis to acute gangrenous mastitis after neutropenia in blood and bone marrow produced by an equine anti-bovine leukocyte serum. Am. J. Vet. Res. 1976, 37, 885-890.
• 20.Scruggs D. W., Fleming S. A., Maslin W. R., Grace A. W.: Osteopetrosis, anemia, thrombocytopenia, and marrow necrosis in beef calves naturally infected with bovine virus diarrhea virus. J. Vet. Diagn. Invest. 1995, 7, 555-559.
• 21.Weber W. T.: Cattle leukemia - hematologic and biochemical studies. Ann. N. Y. Acad. Sci. 1963, 108, 1270-1283.
• 22.Weiss D. J., Greig B., Aird B., Geor R. J.: Inflammatory disorders of bone marrow. Vet. Clin. Pathol. 1992, 21, 79-84.
• 23.Weiss D. J., Miller D. C.: Bone marrow necrosis associated with pancytopenia in a cow. Vet. Pathol. 1985, 22, 90-92.
• 24.Wilde J. K. H.: The cellular elements of the bovine marrow. Res. Vet. Sci. 1964, 5, 227-231.

Uwagi

Rekord w opracowaniu