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2012 | 21 | 2 |

Tytuł artykułu

Ultrasonic stimulation of co-immobilized saccharomyces cerevisiae cells and β-galactosidase enzyme for enhanced ethanol production from whey ultrafiltration permeate

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Low energy ultrasound irradiation (20 kHz, 1.0 W‧L⁻¹) was applied to enhance bioethanol production from whey ultrafiltration permeate by co-immobilized Saccharomyces cerevisiae cells and β-galactosidese enzyme. Sugar utilization and ethanol formation were investigated as a function of hydraulic retention time (HRT) between 12 and 36 h. Maximum ethanol production under HRT of 36 h was 26.30 g‧L⁻¹ with ultrasound exposure, and 23.60 g‧L⁻¹ without. Maximum ethanol yield was 0.532 g‧g⁻¹ lactose in the fermentation process with ultrasound irradiation, and 0.511 g‧g⁻¹ without. For the continuously operating bioreactors, the maximum rates of sugar utilization were 98.9 and 92.4% for the yeast with and without ultrasound exposure, respectively. These results highlight the positive effect of low-intensity ultrasounds in bioethanol fermentation from whey permeates, raising new perspectives for its disposal.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

21

Numer

2

Opis fizyczny

p.387-393,fig.,ref.

Twórcy

  • Department of Environmental Protection Engineering, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1, 10-957 Olsztyn, Poland

Bibliografia

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  • 3. SILVEIRA W. B., PASSOS F. J. V., MANTOVANI H. C., PASSOS F. M. L. Ethanol production from cheese whey permeate by Kluyveromyces marxianus UFV-3: A flux analysis of oxido-reductive metabolism as a function of lactose concentration and oxygen levels. Enzyme Microb. Technol. 36, 930, 2005.
  • 4. KOURKOUTAS Y., DIMITROPOULOU S., KANELLAKI M., MARCHANT R., NIGAM P., BANAT I. M. Hightemperature alcoholic fermentation of whey using Kluyveromyces marxianus IMB3 yeast immobilized on delignified cellulosic material. Bioresour. Technol. 82, 177, 2002.
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  • 6. COTÉ A., BROWN W. A., CAMERON D., van WALSUM G. P. Hydrolysis of lactose in whey permeate for subsequent fermentation to ethanol. J. Dairy Sci. 87, 1608, 2004.
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  • 11. LIU Y., TAKATSUKI H., YOSHIKOSHI A., WANG B., SAKANISHI A. Effects of ultrasound on the growth and vacuolar H+-ATPase activity of aloe arborescens callus cells. Colloids Surf. B 32, 105, 2003.
  • 12. LIU H., YAN Y., WANG W., YU Y. Low intensity ultrasound stimulates biological activity of aerobic activated sludge. Front. Energy Power Eng. China 1, 67, 2007.
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  • 16. MARQUES L. L. M., BUZATO J. B., CELLIGOI M. A. P. C. Effect of raffinose and ultrasound pulses on invertase release by free and immobilized Saccharomyces cerevisiae in loofa (Luffa cylindrica) sponge. Braz. Arch. Biol. Technol. 49, 1516, 2006.
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  • 24. LIU Y., YOSHIKOSHI A., WANG B. C., SAKANISHI A. Influence of ultrasonic stimulation on the growth and proliferation of Oryza sativa Nipponbare callus cells. Colloids Surf. B 27, 287, 2003.
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Typ dokumentu

Bibliografia

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Identyfikator YADDA

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