Differences in metabolic profiles of planktonic and biofilm cells in Staphylococcus aureus-1H Nuclear Magnetic Resonance search for candidate biomarkers

• Autorzy: Junka A. F. , Deja S. , Smutnicka D. , Szymczyk P. , Ziolkowski G. , Bartoszewicz M. , Mlynarz P.
• Języki publikacji: EN

Abstrakty

EN

Staphylococcus aureus is responsible for many types of infections related to biofilm presence. As the early diagnostics remains the best option for prevention of biofilm infections, the aim of the work presented was to search for differences in metabolite patterns of S. aureus ATCC6538 biofilm vs. free-swimming S. aureus planktonic forms. For this purpose, Nuclear Magnetic Resonance (NMR) spectroscopy was applied. Data obtained were supported by means of Scanning Electron Microscopy, quantitative cultures and X-ray computed microtomography. Metabolic trends accompanying S. aureus biofilm formation were found using Principal Component Analysis (PCA). Levels of isoleucine, alanine and 2,3-butanediol were significantly higher in biofilm than in planktonic forms, whereas level of osmoprotectant glycine-betaine was significantly higher in planktonic forms of S. aureus. Results obtained may find future application in clinical diagnostics of S. aureus biofilm-related infections.

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2013
• Tom: 60
• Numer: 4
• Opis fizyczny: p.710-706,fig.,ref.

Twórcy

autor

Junka A. F.

• Department of Microbiology, Medical University of Wroclaw, Wroclaw, Poland

autor

Deja S.

• Faculty of Chemistry, Opole University, Opole, Poland

autor

Smutnicka D.

• Department of Microbiology, Medical University of Wroclaw, Wroclaw, Poland

autor

Szymczyk P.

• Wroclaw University of Technology, Centre for Advanced Manufacturing Technologies, Wroclaw, Poland

autor

Ziolkowski G.

• Wroclaw University of Technology, Centre for Advanced Manufacturing Technologies, Wroclaw, Poland

autor

Bartoszewicz M.

• Department of Microbiology, Medical University of Wroclaw, Wroclaw, Poland

autor

Mlynarz P.

• Faculty of Chemistry, Opole University, Opole, Poland

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