Kształtowanie się cech morfologicznych i biochemicznych łubinu żółtego rosnącego na glebie zanieczyszczonej chlorotetracykliną w warunkach fotoperiodu i ciemności

• Autorzy: Baciak M. , Piotrowicz-Cieslak A.I. , Adomas B.

Warianty tytułu

EN

Morphological and biochemical characteristics of yellow lupin growing on soil contaminated with chlortetracycline in photoperiod and darkness

• Języki publikacji: PL

Abstrakty

PL

W ocenie zanieczyszczenia środowiska lekami bardzo ważną rolę odgrywają badania z wykorzystaniem roślin przeznaczonych na cele konsumpcyjne. Celem pracy było określenie fitotoksyczności chlorotetracykliny (0, 1,45, 2,90, 5,80, 11,60, 23,19 i 46,38 mg∙kg-1 gleby) obecnej w glebie wobec łubinu żółtego (Lupinus luteus L.) odmiany Mister. Oceniano wybrane cechy morfologiczne łubinu oraz biochemiczne aktywności peroksydazy i katalazy w korzeniach i łodygach siewek łubinu w warunkach fotoperiodu i ciemności. Stwierdzono, że niskie stężenia chlorotetracykliny w glebie nie zmieniają długości korzeni i łodyg. Ciśnienie osmotyczne siewek łubinu w obu wariantach świetlnych przy najwyższym stężeniu leku (46,38 mg∙kg-1 gleby) wzrosło około dwukrotnie w porównaniu z próbami kontrolnymi. Aktywność peroksydazy korzeni rosnących na glebie zawierającej lek w stężeniu 46,38 mgkg-1wyniosła 29 U (fotoperiod) i 24 U (ciemność). Aktywność katalazy była większa w łodygach łubinu żółtego rosnących w ciemności (15 U) w porównaniu do korzeni rosnących w tych samych warunkach świetlnych (12 U).

EN

Veterinary antibiotics are important measures for the treatment of diseases in production animals. However, the annual increase in their use in cattle and poultry production is disturbing.Tetracyclines, including tetracycline, chlortetracycline and oxytetracycline, are among the most commonly used antibiotics worldwide. Since they are poorly absorbed and excreted in substantial amounts in the digestive tract of animals (about 5080%) with faeces and urine, chlortetracycline, oxytetracycline and tetracycline are detected in manure and slurry. They are sensitive to light and are degraded by light and oxidative factors. The studies were conducted under photoperiodic conditions and constant darkness in order to establish whether solar light has an impact on the uptake of chlortetracycline by 8-day-old yellow lupin seedlings. In addition, it was investigated whether chlortetracycline in soil (under photoperiod or darkness) influences the activity of peroxidase and superoxide dismutase, i.e. the enzymes that neutralize free radicals, in yellow lupin roots and stems. Research using plants typically intended for consumption plays a very important role in the evaluation of environment contaminated with drugs. The aim of the study was to investigate the phytotoxicity low concentration of chlortetracycline (0, 1.45, 2.90, 5.80, 11.60, 23.19 and 46.38 mg-kg-1 of soil) to yellow lupin (Lupinus luteus L.) cv. Mister. Morphological characteristics and biochemical reactions (peroxidase and catalyze activities) yellow lupin seedlings growing in a photoperiod and in darkness were evaluated. It was found that chlo-rtetracycline was phytotoxic to yellow lupin. The average length of roots growing on soil contaminated with the tested concentrations of chlortetracycline was 76 mm (photoperiod) and 82 mm (darkness). Lupin produced significantly shorter roots compared to the control growing in darkness on soil contaminated with 1.45, 2.90, and 5.80 mg-kg-1 of soil. It was found that low concentrations of chlortetracycline in soil were not changed roots and stems length, and in contrast to the dark photoperiod to prolong the test organs. The osmotic pressure of lupin seedlings in both light conditions at the highest concentration of the drug (46.38 mg∙kg-1 of soil) was increased about twice compared with controls. Peroxidase activity was achieved the value of 29 U (photoperiod) and 24 U (dark) at the highest concentration of the drug (46.38 mg∙kg-1 of soil). Catalase activity was higher in the stems of yellow lupin grown in the dark (15 U) compared to the roots grown in the same conditions of light (12 U).

• Wydawca: -
• Czasopismo: Zeszyty Problemowe Postępów Nauk Rolniczych
• Rocznik: 2015
• Tom: 583
• Opis fizyczny: s.3-11,rys.,bibliogr.

Twórcy

autor

Baciak M.

• Katedra Chemii, Wydział Kształtowania Środowiska i Rolnictwa, Uniwersytet Warmińsko-Mazurski w Olsztynie

autor

Piotrowicz-Cieslak A.I.

• Uniwersytet Warmińsko-Mazurski w Olsztynie

autor

Adomas B.

• Uniwersytet Warmińsko-Mazurski w Olsztynie

Bibliografia

• Błaszczak M., Przybulewska K., 2011. Wpływ pestycydów triazynowych na mikroorganizmy glebowe. Zesz. Probl. Post. Nauk Rol. 567, 21-28.
• Bowler C., Camp V.W., Montagu V., Inzé D., 1992. Superoxide dismutase and stress tolerance. Annu. Rev. Plant Physiol. Plant Mol. Biol. 43, 83-116.
• Cosio C., Dunand C., 2009. Specific functions of individual class III peroxidase genes. J. Exp. Bot. 60(2), 391-408.
• Fang H., Han Y.L., Yin Y.M., Pan X., Yu Y.L., 2014. Variations in dissipation rate, microbial function and antibiotic resistance due to repeated introductions of manure containing sulfadia-zine and chlortetracycline to soil. Chemosphere 96, 51-56.
• Grace S.C., Logan B.A., 2000. Energy dissipation and radical scavenging by the plant phenylpropanoid pathway. Philos. Trans. R Soc. Lond. B. Biol. Sci. 355(1402), 1499-1510.
• Jiao S., Zheng S., Yin D., Wang L., Chen L., 2008. Aqueous photolysis of tetracycline and toxicity of photolytic products to luminiscent bacteria. Chemosphere 73(3), 377-382.
• Kaczyńska G., Szudrewicz A., Hawryluk U., Strachel R., 2011. Reakcja drobnoustrojów i enzymów na zanieczyszczenie gleby miedzią. Zesz. Probl. Post. Nauk Rol. 567, 105-116.
• Kim Y.K., Lim S.J., Han M.H., Cho J.Y., 2012. Sorption characteristics of Oxytetracycline, amoxicillin, and sulfathiazole in two different soil types. Geoderma 185-186, 97-101.
• Kong W., Li Ch., Dolhi J.M., Li S., He J., Qiao M., 2012. Characteristics of oxytetracycline sorption and potential bioavailability in soils with various physical-chemical properties. Che-mosphere 87(5), 542-548.
• Liu L., Liu Y., Liu Ch., Wang Z., Dong J., Zhu G., Huang X., 2012. Potential effect and accumulation of veterinary antibiotics in Phragmites australis under hydroponic conditions. Ecol. Eng. 53, 138-143.
• Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J., 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265-275.
• de Maria N., de Felipe M.R., Fernandez-Pascual M., 2005. Alternation induced by glyphosate on lupin photosynthetic apparatus and nodule ultrastructure and some oxygen diffusion related proteins. Plant Physiol. Biochem. 43(10-11), 985-996.
• Marjamaa K., Kukkola E.M., Fagerstedt K.V., 2009. The role of xylem class III peroxidases in lignification. J. Exp. Bot. 60(2), 367-376.
• Mathé C., Barre A., Jourda C., Dunand C., 2010. Evolution and expression of class III peroxidases Arch. Biochem. Biophys. 500(1), 58-65.
• Passardi F., Cosio C., Penel C., Dunand C., 2005. Peroxidases have more functions than a Swiss army knife. Plant Cell Rep. 24(5), 255-265.
• Pereira L.B., de A. Mazzanti C.M., Gonęalves J.F., Cargnelutti D., Tabaldi L.A., Becker A.G., Cal-garoto N.S., Farias J.G., Battisti V., Bohrer D., Nicoloso F.T., Morsch V.M., Schetinger M.R.C., 2010. Aluminum-induced oxidative stress in cucumber. Plant Physiol. Bioch. 48(8), 683- 689.
• Qin P., Liu R., 2013. Oxidative stress response of two fluoroquinolones with catalase and erythrocytes: A combined molecular and cellular study. J. Hazard. Mater. 252-253, 321-329.
• Sikorski Ł., Adomas B., Dobiesz M., Baciak M., Piotrowicz-Cieślak A.I., 2014. Morphological and biochemical responses of Lemna minor L. (common duckweed) to ciprofloxacin. Fresen. Environ. Bull. 23(2), 363-371.
• Wang Y., Sun R., Xiao A., Wang S., Zhou D., 2011. Phosphate affects the adsorption of tetracycline on two soils with different characteristics. Geoderma 156(3-4), 237-242.
• Wu X., Wei Y, Zheng J., Zhao X., Zhong W., 2011. The behavior of tetracyclines and their degradation products during swine manure composting. Bioresource Technol. 102(10), 5924-5931.
• Zhao-jun Li, Xiao-yu X., Shu-qing Z., Yong-chao L., 2011. Negative effects of oxytetracycline on wheat (Triticum aestivum L.) growth, root activity, photosynthesis, and chlorophyll contents. Agricultural Sciences in China 10(10), 1545-1553.
• Ziółkowska A., Piotrowicz-Cieślak A.I., Rydzyński D., Adomas B., Nałęcz Jawecki G., 2014. Bio-markers of leguminous plant viability in response to soil contamination with diclofanac. Pol. J. Environ. Stud. 23(1), 263-269.