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2014 | 36 | 02 |

Tytuł artykułu

Genetic stability in micropropagated Cleome gynandra revealed by SCoT analysis

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
A newly developed and novel DNA marker technique, i.e. start codon targeted (SCoT) polymorphic markers that target plant gene regions were used to assess genetic stability of in vitro raised plants of Cleome gynandra multiplied by enhanced bud proliferation from nodal segments. Seven randomly selected micropropagated plants, following at least 2 months of growth in the greenhouse along with mother plant were subjected to molecular analysis. Of 24 primers screened, 15 primers produced unambiguous and reproducible bands. All 15 primers generated a total of 65 fragments, with a mean of 4.3 ranging 2–7 per primer. No polymorphism was detected in regenerated plants and the mother plant, revealing the genetic fidelity of the in vitro raised plantlets. To verify the results of SCoT analysis, random amplified polymorphic DNA (RAPD) markers were also used for the assessment of genetic fidelity of tissue culture raised plants. The monomorphic banding pattern in micropropagated plants and the mother plant obtained from SCoT and RAPD analysis confirms the genetic stability of the in vitro raised plants and demonstrates the reliability of our micropropagation system for C. gynandra, an important C₄ plant.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

36

Numer

02

Opis fizyczny

p.555-559,fig.,ref.

Twórcy

autor
  • Department of Botany, Biotechnology Centre, Jai Narain Vyas University, Jodhpur, 342033, Rajasthan, India
autor
  • Department of Botany, Biotechnology Centre, Jai Narain Vyas University, Jodhpur, 342033, Rajasthan, India
autor
  • Department of Botany, Biotechnology Centre, Jai Narain Vyas University, Jodhpur, 342033, Rajasthan, India
autor
  • Department of Botany, Biotechnology Centre, Jai Narain Vyas University, Jodhpur, 342033, Rajasthan, India
  • Department of Botany, Biotechnology Centre, Jai Narain Vyas University, Jodhpur, 342033, Rajasthan, India

Bibliografia

  • Amirmoradi B, Talebi R, Karami E (2012) Comparison of genetic variation and differentiation among annual Cicer species using start codon targeted (SCoT) polymorphism, DAMD-PCR, and ISSR markers. Plant Syst Evol 298:1679–1688.
  • Asthana P, Jaiswal VS, Jaiswal U (2011) Micropropagation of Sapindus trifoliatus L. and assessment of genetic fidelity of micropropagated plants using RAPD analysis. Acta Physiol Plant 33:1821–1829.
  • Bairu MW, Aremu AO, Van Staden J (2011) Somaclonal variation in plants: causes and detection methods. Plant Growth Regul 63:147–173.
  • Bhojwani SS, Dantu PK (2013) Plant tissue culture: an introductory text. Springer, India. doi:10.1007/978-81-322-1026-9.
  • Collard BCY, Mackill DJ (2009) Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant Mol Biol Rep 27:86–93.
  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15.
  • Gorji AM, Poczai P, Polgar Z, Taller J (2011) Efficiency of arbitrarily amplified dominant markers (SCoT, ISSR and RAPD) for diagnostic fingerprinting in tetraploid potato. Am J Pot Res 88:226–237.
  • Guo DL, Zhang JY, Liu CH (2012) Genetic diversity in some grape varieties revealed by SCoT analyses. Mol Biol Rep 39:5307–5313.
  • Kumar S, Mangal M, Dhawan AK, Singh N (2011) Assessment of genetic fidelity of micropropagated plants of Simmondsia chinensis (Link) Schneider using RAPD and ISSR markers. Acta Physiol Plant 33:2541–2545.
  • Luo C, He XH, Chen H, Hu Y, Ou SJ (2012) Genetic relationship and diversity of Mangifera indica L.: revealed through SCoT analysis. Genet Resour Crop Evol 59:1505–1515.
  • Nayak SA, Kumar S, Satapathy K, Moharana A, Behera B, Barik DP, Acharya L, Mohapatra PK, Jena PK, Naik SK (2013) In vitro plant regeneration from cotyledonary nodes of Withania somnifera (L.) Dunal and assessment of clonal fidelity using RAPD and ISSR markers. Acta Physiol Plant 35:195–203.
  • Negi D, Saxena S (2010) Ascertaining clonal fidelity of tissue culture raised plants of Bambusa balcooa Roxb. Using inter simple sequence repeat markers. New Forest 40:1–8.
  • Phulwaria M, Rai MK, Shekhawat NS (2013a) An improved micropropagation of Arnebia hispidissima (Lehm.) DC. and assessment of genetic fidelity of micropropagated plants using DNA based molecular markers. Applied Biochem Biotechnol 170:1163–1173.
  • Phulwaria M, Rai MK, Patel AK, Kataria V, Shekhawat NS (2013b) A genetically stable rooting protocol for propagating a threatened medicinal plant-Celastrus paniculatus. AoB Plants. doi:10.1093/aobpla/pls054.
  • Rai MK, Phulwaria M, Harish, Gupta AK, Shekhawat NS, Jaiswal U (2012) Genetic homogeneity of guava plants derived from somatic embryogenesis using SSR and ISSR markers. Plant Cell Tissue Org Cult 111:259–264.
  • Rani V, Raina SN (2000) Genetic fidelity of organized meristem derived micropropagated plants: a critical reappraisal. In Vitro Cell Dev Biol Plant 36:319–330.
  • Rathore MS, Chikara J, Mastan SG, Rahman H, Anand KVG, Shekhawat NS (2011) Assessment of genetic stability and instability of tissue culture-propagated plantlets of Aloe vera L. by RAPD and ISSR markers. Applied Biochem Biotechnol 165:1356–1365.
  • Rathore NS, Rathore N, Shekhawat NS (2013) In vitro propagation and micromorphological studies of Cleome gynandra: a C₄ model plant closely related to Arabidopsis thaliana. Acta Physiol Plant 35:2691–2698.
  • Singh SK, Rai MK, Sahoo L (2012) An improved and efficient micropropagation of Eclipta alba through transverse thin cell layer culture and assessment of clonal fidelity using RAPD analysis. Indust Crop Prod 37:328–333.
  • Singh SR, Dalal S, Singh R, Dhawan AK, Kalia RK (2013) Evaluation of genetic fidelity of in vitro raised plants of Dendrocalamus asper (Schult. and Schult. F.) Backer ex K. Heyne using DNA-based markers. Acta Physiol Plant 35:419–430.
  • Sujatha M, Tarakeswari M, Francis G (2013) Start codon targeted (SCoT) polymorphism in toxic and non-toxic accessions of Jatropha curcas L. and development of a codominant SCAR marker. Plant Sci 207:117–127.
  • Xiong FQ, Zhong RC, Han ZQ, Jiang J, He LQ, Zhuang WJ, Tang RH (2011) Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated peanut (Arachis hypogaea L.) genotypes. Mol Biol Rep 38:3487–3494.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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