Thiamine pyrophosphate deficiency causes energy disturbances in astroglial C6 and microglial N9 cells

• Autorzy: Romowska A. , Bizon-Zygmanska D. , Gul-Hinc S. , Dys A. , Klimaszewska-Lata J. , Bielarczyk H. , Wolska A. , Szutowicz A.
• Języki publikacji: EN

Abstrakty

EN

Inhibition of pyruvate (PDHC) and ketoglutarate (KDHC) dehydrogenase complexes induced by thiamine pyrophosphate deficits is known to cause disturbances of cholinergic transmission in the brain, yielding clinical symptoms of cognitive and motor deficits. However, particular alterations in distribution of acetyl-CoA, in the glial cells of thiamine pyrophosphate-deficient brain remain unknown. Therefore, the aim of our work was to find out how amprolium-induced thiamine pyrophosphate deficits (TD) affect distribution of acetyl-CA in the compartments of glial cells. As an experimental model we used astroglial C6 and microglial N9 cell line cultured in low thiamine medium. In such conditions microglial N9 cells displayed significantly greater loss of viability than the C6 ones. In both groups of the cells the activity of the key enzymes of energy/acetyl-CoA metabolism such as: PDHC, KDHC, aconitase was inhibited by amprolium-induced thiamine deficits. It explains why acetyl-CoA levels in the mitochondrial compartment were decreased in the cells. Supported by the Ministry of Research and Higher Education projects: IP 2011 046071, 01-0100/08 and St 57.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2013
• Tom: 73
• Numer: Suppl.1
• Opis fizyczny: p.64

Twórcy

autor

Romowska A.

• Department of Laboratory Medicine, Chair of Clinical Biochemistry, Medical University of Gdansk, Gdansk, Poland

autor

Bizon-Zygmanska D.

• Department of Laboratory Medicine, Chair of Clinical Biochemistry, Medical University of Gdansk, Gdansk, Poland

autor

Gul-Hinc S.

• Department of Laboratory Medicine, Chair of Clinical Biochemistry, Medical University of Gdansk, Gdansk, Poland

autor

Dys A.

• Department of Laboratory Medicine, Chair of Clinical Biochemistry, Medical University of Gdansk, Gdansk, Poland

autor

Klimaszewska-Lata J.

• Department of Laboratory Medicine, Chair of Clinical Biochemistry, Medical University of Gdansk, Gdansk, Poland

autor

Bielarczyk H.

• Department of Laboratory Medicine, Chair of Clinical Biochemistry, Medical University of Gdansk, Gdansk, Poland

autor

Wolska A.

• Department of Laboratory Medicine, Chair of Clinical Biochemistry, Medical University of Gdansk, Gdansk, Poland

autor

Szutowicz A.

• Department of Laboratory Medicine, Chair of Clinical Biochemistry, Medical University of Gdansk, Gdansk, Poland