Transcript expression and soluble acid invertase activity during sucrose accumulation in sugarcane

• Autorzy: Verma A.K. , Upadhyay S. K. , Srivastava M. K. , Verma P. C. , Salomon S. , Singh S. B.
• Języki publikacji: EN

Abstrakty

EN

Soluble acid invertase (SAI, EC 3.2.1.26), catalyzes the hydrolysis of sucrose into hexose sugars, and it has been considered a key enzyme for carbohydrate metabolism. In the present study, the activity of SAI enzyme was determined to establish a correlation between the change in transcript levels and enzyme activity in high and low sugar accumulating sugarcane cultivars, in various internodal tissues at different developmental stages. A decrease in SAI activity and transcript levels was observed with age, during all the developmental stages in both the cultivars. A negative correlation between SAI activity and sucrose content was observed in mature and immature internodes; however, there was a positive correlation between SAI activity and content of hexose sugars. These results imply that SAI plays a crucial role in sucrose partitioning in various intermodal tissues in high and low sugar cultivars. In addition to this, the changes in enzyme activity also resulted in changes in transcript level.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 5
• Opis fizyczny: p.1749-1757,fig.,ref.

Twórcy

autor

Verma A.K.

• Plant Molecular Biology Laboratory, National Botanical Research Institute, Lucknow, 226001, India

autor

Upadhyay S. K.

• Plant Molecular Biology Laboratory, National Botanical Research Institute, Lucknow, 226001, India

autor

Srivastava M. K.

• Key Laboratory of Genetic Crop Improvement and Biotechnology, Guangxi Academy of Agricultural Sciences, Nanning, 530007, Guangxi, People s Republic of China

autor

Verma P. C.

• Plant Molecular Biology Laboratory, National Botanical Research Institute, Lucknow, 226001, India

autor

Salomon S.

• Indian Institute of Sugarcane Research, Lucknow, India

autor

Singh S. B.

• U.P. Council of Sugarcane Research, Shahjahanpur, 242001, India

Bibliografia

• Batta SK, Singh R (1986) Sucrose metabolism in sugarcane grown under varying climatic conditions: synthesis and storage of sucrose in relation to the activities of sucrose synthase, sucrose phosphate synthase and invertase. Phytochemistry 25:2431–2437
• Botha FC, Black KG (2000) Sucrose phosphate synthase and sucrose synthase activity during maturation of internodal tissue in sugarcane. Aust J Plant Physiol 27:81–85
• Burg SP, Bieleski RL (1962) The physiology of sugarcane. V. Kinetics of sugar accumulation. Aust J Biol Sci 15:429–444
• Carson DL, Botha FC (2002) Genes expressed in sugarcane maturing internodal tissue. Plant Cell Rep 20:1075–1081
• Carson DL, Huckett BI, Botha FC (2002) Sugarcane ESTs differentially expressed in immature and maturing internodal tissue. Plant Sci 162:289–300
• Casu RE, Jarmey JM, Bonnett GD, Manners JM (2007) Identification of transcripts associated with cell wall metabolism and development in the stem of sugarcane by Affymetrix GeneChip Sugarcane Genome Array expression profiling. Funct Integr Genomics 7:153–167
• Echeverria E, Gonzalez PC, Brune A (1997) Characteristics of proton and sugar transport at the tonoplast of sweet lime (Citrus limettioides) juice cells. Physiol Plant 101:291–300
• Glasziou KT, Gayler KR (1972) Storage of sugars in stalks of sugarcane. Bot Rev 38:471–490
• Grof CPL, Campbell JA (2001) Sugarcane sucrose metabolism: scope for molecular manipulation. Aust J Plant Physiol 28:1–12
• Hawker JS, Hatch MD (1965) Mechanism of sugar storage by mature stem tissue of sugarcane. Plant Physiol 18:444–453
• Klann EM, Hall B, Bennett AB (1996) Antisense acid invertase (T1V1) gene alters soluble sugar composition and size in transgenic tomato fruit. Plant Physiol 112:1321–1330
• Kubo T, Hohjo I, Hiratsuka S (2001) Sucrose accumulation and its related enzyme activities in the juice sacs of Satsuma mandarin fruit from trees of different crop loads. Sci Hortic 91:215–225
• Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275
• Ma H, Albert HH, Paull R, Moore PH (2000) Metabolic engineering of invertase activities in different subcellular compartments affects sucrose accumulation in sugarcane cells. Aust J Plant Physiol 27:1021–1030
• Martinoia E (1992) Transport process in vacuoles of higher plants. Bot Acta 105:232–245
• McCormick AJ, Cramer MD, Watt DA (2006) Sink strength regulates photosynthesis in sugarcane. New Phytol 171:759–770
• Menossi M, Silva-Filho MC, Vincentz M, Van-Sluys MA, Souza GM (2008) Sugarcane functional genomics: gene discovery for agronomic trait development. Int J Plant Genomics 1:11
• Moore PH (1995) Temporal and spatial regulation of sucrose accumulation in the sugarcane stem. Aust J Plant Physiol 22:661–679
• Moore PH (2005) Integration of sucrose accumulation processes across hierarchical scales: towards developing an understanding of the gene to crop continuum. Field Crop Res 92:119–135
• Nelson N (1944) A photometric adaptation of Somogyi method for the determination of glucose. J Biol Chem 153:375–380
• Quick WP, Schaffer AA (1996) Sucrose metabolism in sources and sinks. In: Zamski E, Schaffer AA (eds) Photoassimilate distribution in plants and crops. Marcel Dekker, NY, pp 115–156
• Rausch T, Greiner S (2004) Plant protein inhibitors of invertases. Biochim Biophys Acta 1696:253–261
• Roe JH (1934) A colorimetric method for the determination of fructose in blood and urine. J Biol Chem 107:15–22
• Sacher JA, Hatch MD, Glasziou KT (1963) Sugar accumulation cycle in sugarcane. III. Physical and metabolic aspect of cycle in immature storage tissues. Plant Physiol 39:348–354
• Scholes J, Bundock N, Wilde R, Rolfe S (1996) The impact of reduced vacuolar invertase on the photosynthetic and carbohydrate metabolism of tomato. Planta 200:265–272
• Singh RK, Singh P, Singh SP, Mohapatara T, Singh SB (2006) Mapping QTLs for sugar content and segregation analysis in sugarcane. Sugar Cane Int 24:7–13
• Tang GQ, Luscher M, Sturm A (1999) Antisense repression of vacuolar and cell wall invertase in transgenic carrot alters early plant development and sucrose portioning. Plant Cell 11:177–190
• Vattuone MA, Fleischmacher OL, Prado FE, Vinals AL, Sampietro AR (1983) Localisation of invertase activities in Ricinus communis leaves. Phytochemistry 22:1361–1365
• Venkataramana S, Naidu MK, Singh S (1991) Invertase and growth factors dependent sucrose accumulation in sugarcane. Plant Sci 74:65–72
• Verma AK, Upadhyay SK, Verma PC, Solomon S, Singh SB (2010) Functional analysis of sucrose phosphate synthase (SPS) and sucrose synthase (SS) in sugarcane (Saccharum) cultivars. Plant Biol. doi:10.1111/j.1438-8677.2010.00379.x
• Walker RP, Pollock CJ (1993) The purification and characterisation of soluble acid invertase from coleoptiles of wheat (Triticum aestivum L. cv. Avalon). J Exp Bot 44:1029–1037
• Welbaum GE, Meinzer FC (1990) Compartmentation of solutes and water in developing sugarcane stalk tissue. Plant Physiol 93:1147–1153
• Whittaker A, Botha FC (1997) Carbon partitioning during sucrose accumulation in sugarcane internodal tissues. Plant Physiol 115:1651–1659
• Winter H, Robinson DG, Heldt HW (1994) Subcellular volumes and metabolites in spinach leaves. Planta 193:530–535
• Yamada K, Kojima T, Bantog N, Shimoda T, Mori H, Shiratake K, Yamaki S (2007) Cloning of two isoforms of soluble acid invertase of Japanese pear and their expression during fruit development. J Plant Physiol 164:746–755
• Zhu YJ, Komor E, Moore PH (1997) Sucrose accumulation in the sugarcane stem is regulated by the difference between the activities of soluble acid invertase and sucrose phosphate synthase. Plant Physiol 115:609–616
• Zhu YJ, Albert HH, Moore PH(2000) Differential expression of soluble acid invertase (SAI) genes correlates to differences in sucrose accumulation in sugarcane. Aust J Plant Physiol 27:193–199

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