Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 36 | 03 |

Tytuł artykułu

Changes in some anti-oxidative enzymes and physiological indices among sesame genotypes (Sesamum indicum L.) in response to soil water deficits under field conditions

Warianty tytułu

Języki publikacji



A field experiment was conducted to evaluate the response of ten sesame genotypes to different levels of soil water in terms of contents of proline, soluble carbohydrates, carotenoids, and activities of catalase (CAT), peroxidase (POX) and ascorbate peroxidase (APX). Plants were grown under three irrigation levels, including irrigation at 55 % (control), 75, and 85 % depletion of soil available water. Field test plots were a two-way factorial arranged in a randomized complete block design with three replications. Under control level of irrigation, the most and the least grain yields were achieved for genotypes Ultan (2,519 kg/ha) and Isfahan1 (1,311 kg/ha), respectively. Grain yield was decreased in some genotypes under 75 % and in all genotypes under 85 % depletion of available water. Based on percentage reduction in grain yield under both 75 and 85 % depletion of soil available water, Isfahan4, Borazjan, Isfahan1, Ahvaz, Ardestan, and Shiraz were recognized as relatively tolerant and Ultan, Shahreza, Kal, and Markazi were identified as relatively sensitive to water stress. The activities of antioxidant enzymes and the contents of carotenoids, proline, and soluble carbohydrates in leaves were increased in most genotypes under stress conditions, and the magnitudes of the increases were greater in the tolerant than in the sensitive genotypes. The results of this experiment showed that the stress-induced increase of antioxidant enzymes and the contents of the compatible solutes in leaves were related to the tolerance of sesame genotypes.

Słowa kluczowe








Opis fizyczny



  • Department of Agronomy and Plant Breeding, Isfahan University of Technology, Isfahan, Iran
  • Department of Agronomy and Plant Breeding, Isfahan University of Technology, Isfahan, Iran
  • Department of Agronomy and Plant Breeding, Isfahan University of Technology, Isfahan, Iran
  • School of Plant Sciences, The University of Arizona, Tucson, AZ, 85721, USA


  • Abedi T, Pakniyat H (2010) Antioxidant enzyme changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.). Plant Breed 46:27–34.
  • Alizadeh A (2004) Soil, water and plant relationship, 4th edn. Emam Reza University Press, Mashhad (ISBN. 964-6582-57-50).
  • Allen RG, Pereira LS, Raes D, Smith M (2000) FAO irrigation and drainage paper. Crop Evapotranspiration (Guidel Comput Crop Water Requir) 56:1–326.
  • Andrew JS, Moreau H, Kuntz M, Pagny G, Lin C, Tanksley S, McCarthy J (2008) An investigation of carotenoid biosynthesis in Coffea canephora and Coffea arabica. Plant Physiol 165:1087–1106.
  • Bartoli CG, Simontacchi M, Tambussi E, Beltrano J, Montaldi E, Puntarulo S (1999) Drought and watering-dependent oxidative stress: effect on antioxidant content in Triticum aestivum L. leaves. J Exp Bot 50:375–383.
  • Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39(1):205–207.
  • Boydak E, Karaaslan D, Simsek M, Gercak S, Kirnak H, Kaspa Y, Ozturk I (2007) Effect of irrigation methods and irrigation interval on yield and some yield components of sesame growing in semi-arid area. J Agron 6(3):439–443.
  • Brar GS, Kar S, Singh NT (1990) Photosynthetic response of wheat to soil water deficits in the tropics. J Agron Crop Sci 164:343–348.
  • Chance B, Maehly AC (1955) Assay of catalases and peroxidase. Method Enzymol 2:764–775.
  • Demmig-Adams B, Adams WW (1996) Chlorophyll and carotenoid composition in leaves of Euonymus kiautschovicus acclimated to different degrees of light stress in the field. Aust J Plant Physiol 23:649–659.
  • Deng X, Hu ZA, Wang HX, Wen XG, Kuang TY (2003) A comparison of photosynthetic apparatus of the detached leaves of the resurrection plant Boea hygrometrica with its non-tolerant relative Chirita heterotricha in response to dehydration and rehydration. Plant Sci 165:851–861.
  • Fazeli F, Ghorbanli M, Niknam V (2007) Effect of drought on biomass, protein content, lipid peroxidation and anti-oxidant enzymes in two sesame cultivars. Biol Plant 51:98–103.
  • Fischer RA, Maurer R (1978) Drought resistance in spring wheat cultivars. 1. Grain yields responses. Aust J Agric Res 29:897–912.
  • Gao J (2006) Experimental techniques of plant physiology. Higher Education Press, Beijing (In Chinese).
  • Ghorbanli M, Ebrahimzadeh H, Sharifi M (2004) Effects of NaCl and mycorrhizal fungi on antioxidative enzymes in soybean. Biol Plant 48:575–581.
  • Glombitza C, Dubuis PH, Thulke O (2004) Crosstalk and differential response to abiotic and biotic stressors reflected at the transcriptional level of effector genes from secondary metabolism. Plant Mol Biol 51:1–19.
  • Hassanzadeh M, Ebadi M, Panahyan-e-Kivi M, Jamaati-e-Somarin SH, Saeidi M, Zabihi-e-Mahmoodabad R (2009) Evaluation of drought stress on relative water content and chlorophyll content of sesame (Sesamum indicum L.) genotypes at early flowering stage. Res J Environ Sci 3(3):345–350.
  • Havaux M, Tardy F (1999) Loss of chlorophyll with limited reduction of photosynthesis as an adaptive response of Syrian barley landraces to high-light and heat stress. Aust J Plant Physiol 26:569–578.
  • Hendry GAF, Price AH (1993) Stress indications: chlorophylls and carotenoids. In: Hendry GAF, Grime JP (eds) Methods in comparative plant ecology, 1st edn. Chapman and Hall, London, pp 148–152.
  • Herzog V, Fahimi H (1973) Determination of the activity of peroxidase. Anal Biochem 55:554–562.
  • Irigoyen JJ, Emerich DW, Sanchez-Diaz M (1992) Water stress induce changes in concentration of proline and total soluble sugar in nodulated alfalfa (Medicago sativa) plants. Physiol Plant 84:55–60.
  • Jaleel CA, Manivannan P, Wahid A, Farooq M, Somasundaram R, Panneerselvam R (2009) Drought stress in plants: a review on morphological characteristics and pigments composition. Int J Agri Biol 11:100–105.
  • Karaaslan D, Boydak E, Gercek S, Simsek M (2007) Influence of irrigation intervals and row spacing on some yield components of sesame grown in Harran region. Asian J Plant Sci 6:623–662.
  • Kholova J, Hash CT, Kocova M, Vadez V (2011) Does a terminal drought tolerance QTL contribute to differences in ROS scavenging enzymes and photosynthetic pigments in pearl millet exposed to drought? Environ Exp Bot 71:99–106.
  • Kim KS, Park SH, Jenks MA (2006) Changes in leaf cuticular waxes of sesame (Sesamum indicum L.) plants exposed to water deficit. J Plant Physiol 164:1134–1143.
  • Kreps JA, Wu YJ, Chang HS (2002) Transcriptome changes for Arabidopsis in response to salt, osmotic, and cold stress. Plant Physiol 130:2129–2141.
  • Mensah JK, Obadoni BO, Eruotor PG, Onome-Irieguna F (2006) Simulated flooding and drought effects on germination, growth, and yield parameters of sesame (Sesamum indicum L.). Afr J Biotech 5(13):1249–1253.
  • Mitra J (2001) Genetics and genetic improvement of drought resistance in crop plants. Curr Sci 80:758–762.
  • Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9(10):490–498.
  • Munne-Bosch S, Alegre L (2000) Changes in carotenoids, tocopherols and diterpenes during drought and recovery, and the biological significance of chlorophyll loss in Rosmarinus officinalis plants. Planta 207:925–931.
  • Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880.
  • Nazarli H, Faraji F, Zardashti MR (2011) Effect of drought stress and polymer on osmotic adjustment and photosynthetic pigments of sunflower. Cercetări Agronomice în Moldova XLIV(1(145)):35–41.
  • Noctor G, Foyer CH (1998) Ascorbate and glutathione. Keeping active oxygen under control. Ann Rev Plant Physiol Mol Biol 49:249–279.
  • Parida AK, Dagaonkar VS, Phalak MS, Umalkar GV, Aurangabadkar LP (2007) Alterations in photosynthetic pigments, protein and osmotic components in cotton genotypes subjected to short-term drought stress followed by recovery. Plant Biotechnol Rep 1:37–48.
  • Pastori GM, Trippi VS (1992) Antioxidative protection in a drought-resistant maize strain during leaf senescence. J Plant Physiol 87:227–231.
  • Pinhero RG, Pao MV, Palyath G, Murr DP, Fletcher RA (2000) Changes in the activities of antioxidant enzymes and their relationship to genetic and paclobutrazol induced chilling tolerance of maize seedlings. J Plant Physiol 114:695–704.
  • Ramachandra Reddy A, Viswanatha Chaitanya K, Vivekanandan M (2004) Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. J Plant Physiol 161:1189–1202.
  • Rudoplh AS, Crowe JH, Crowe LM (1986) Effects of three stabilizing agents-proline, betaine, and trehalose on membrane phospholipids. Arch Biochem Biophys 245:134–143.
  • Saeidi A, Tohidi-Nejad E, Ebrahimi F, Mohammadi-Nejad G, Shirzadi MH (2012) Investigation of water stress on yield and some yield components of sesame genotypes (Sesamum indicum L.) in Jiroft Region. J Appl Sci Res 8(1):243–246.
  • Selote DS, Khanna-Chopra R (2004) Drought-induced spikelet sterility is associated with an inefficient antioxidant defense in rice panicles. Plant Physiol 121:462–471.
  • Shao HB, Liang ZS, Shao MA (2003) Roles of ABA signal transduction during higher plant seed maturation and germination. Forestry Stud China 5(4):42–51.
  • Shao HB, Liang ZS, Shao MA (2005) Impacts of PEG-6000 pretreatment for barley (Hordeum vulgare L.) seeds on the effect of their mature embryo in vitro culture and primary investigation on its physiological mechanism. Colloids Surf B Biointerfaces 41(2–3):73–77.
  • Sharma P, Dubey RS (2005) Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant Growth Regul 46:209–221.
  • Tantawy MM, Ouda SA, Khalil FA (2007) Irrigation optimization for different sesame varieties grown under water stress conditions. J Applied Sci Res 3:7–12.
  • Terzi R, Kadioglu A (2006) Drought stress tolerance and the antioxidant enzyme system in Ctenanthe setosa. Acta Biol Cracov Bot 48(2):89–96.
  • Vasconcelos ACF, Zhang XZ, Ervin EH, Kiehl JD (2009) Enzymatic antioxidant responses to Biostimulants in maize and soybean subjected to drought. Scientia Agricola 66(3):395–402.
  • Wang JR, Li SX, Li KI (2001) Effect of water limited deficit stress during different growth stages on leaf enzymes of winter wheat. Acta Bot Borel Occident Sin 21(1):47–52.
  • Yang D, Shen X, Zhao T, Ma X (2001) Achievement of drought-injury physiology in maize. Crops (In Chinese) 5:1–4.
  • Zhu JK (2001) Plant salt tolerance. Trends Plant Sci 6:66–71.
  • Zhu JK (2003) Regulation of ion homeostasis under salt stress. Curr Opin Plant Biol 6(5):441–445.
  • Zhu JH, Shi HZ, Lee BH (2004) An Arabidopsis homeodomain transcription factor gene, HOS9, mediates cold tolerance through a CBF-independent pathway. PNAS 101(26):9873–9878.

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.