Evaluation of morphometric parameters of three types of oligodendrocytes in the periaqueductal gray matter of rats in different age groups

• Autorzy: Wawrzyniak A. , Jaworska-Adamu J. , Luszczewska-Sierakowska I. , Guz T.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to evaluate selected morphometric parameters of three types of oligodendrocytes on the basis of their morphology. A total of ten male Wistar rats in two age groups, 25 weeks and 140 weeks, were used for the experiments. After the rats were sacrificed by perfusion-fixation, the midbrains with the periaqueductal grey matter were collected and embedded in celloidin. Semi-thin sections were stained with methylene blue, analysed and photographed under a light microscope. The morphometric parameters taken into account were mean cell size, mean perimeter of soma in µm and mean area of soma in µm2 . In addition, the percentages of different types of oligodendrocytes in the measurement area were determined. The results obtained were analysed statistically. In the PAG of the 25-week-old rats oligodendrocytes with light cytoplasm predominated. Their average size was about 7 µm. In the 140-week-old individuals an increase was observed in the number of oligodendrocytes with medium and dark cytoplasm, and their average size was about 6.79 µm. The perimeters of the three types of oligodendrocytes were similar in the two age groups – about 20 µm. There were also no statistically significant differences in the surface area of the three types of oligodendrocytes in the 25- and 140-week-old rats. The average surface area of these cells was about 40 µm2 . Oligodendrocytes have been neglected in research on older individuals and should therefore be analysed in this regard.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2015
• Tom: 71
• Numer: 05
• Opis fizyczny: p.292-297,fig.,ref.

Twórcy

autor

Wawrzyniak A.

• Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin

autor

Jaworska-Adamu J.

• Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin

autor

Luszczewska-Sierakowska I.

• Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin

autor

Guz T.

• Department of Engineering and Food Machinery, University of Life Sciences in Lublin, Doswiadczalna 44, 20-280 Lublin, Poland

Bibliografia

• 1. Bachevalier J., Landis L. S., Walker L. C., Brickso M., Mishkin M., Price D. L.: Aged monkeys exhibit behavioral deficits indicative of widespread cerebraldysfunction. Neurobiol. Aging 1991, 12, 99-111.
• 2. Baumann N., Pham-Dinh D.: Biology of oligodendrocyte and myelin in the mammalian central nervous system. Physiol. Rev. 2001, 81, 871-927.
• 3. Behbehani M. M.: Functional characteristics of the midbrain periaqueductal gray. Progr. Neurobiol. 1995, 46, 575-605.
• 4. Benarroch E. E., Schmeichel A. M., Low P. A., Parisi J. E.: Differential involvement of the periaqueductal gray in multiple system atrophy. Auton. Neurosci.2010, 158, 111-117.
• 5. Borras D., Ferres I., Pumarola M.: Age-related changes in the brain of the dog. Vet. Pathol. 1999, 36, 202-211.
• 6. Bradl M., Lassmann H.: Oligodendrocytes: biology and pathology. Acta Neuropathol. 2010, 119, 37-53.
• 7. Cavallotti D., Cavallotti C., Pescosolido N., Iannetti G. D., Pacella E.: Amorphometric study of age changes in the rat optic nerve. Ophthalmologica 2001,215, 366-371.
• 8. Cho Ik-Hyun, Park Chang-Hyun, Lee Jong-Hwan, Bae Chun-Sik, Ye Sang-Kyu, Lee Beob-Yi, Park Seung-Hwa, Koh Ki-Seok, Kim Jin-Suk, Chang Byung-Joon:Morphometric and Ultrastructural Change of Myelin-Associated Glycoprotein(MAG)-Immunoreactive Oligodendrocytes by Aging. Korean J. ElectronMicroscopy 2006, 36, 119-130.
• 9. Chen L., Lu W., Yang Z., Yang S., Li C., Shi X., Tang Y.: Age-related changes of the oligodendrocytes in rat subcortical white matter. Anat. Rec. 2011, 294,487-493.
• 10. Edgar N., Sibille E.: A putative functional role for oligodendrocytes in mood regulation. Transl. Psychiatry 2012, 1,2, e 109, doi: 10.
• 11. Ling A. E., Leblond C. P.: Investigation of glial cells in semithin sections. II. Variation with age in the numbers of the various glial cell types in rat cortexnd corpus callosum. J. Comp. Neurol. 1973, 149, 73-81.
• 12. Ling A. E., Paterson J. A., Privat A., Mori S., Leblond C. P.: Investigation of glial cells in semithin sections. I. Identification of glial cells in the brain ofyoung rats J. Comp. Neurol. 1973, 149, 43-71.
• 13. Loyd D. R., Murphy A. Z.: The role of the periaqueductal gray in the modulation of pain in males and females: are the anatomy and physiology really that different? Neural. Plast. 2009, 462879, doi: 10.1155/2009/462879.
• 14. Monteiro R. A., Conceição L. E., Rocha E., Marini-Abreu M. M.: Age changes in cerebellar oligodendrocytes: the appearance of nuclear filaments and increase in the volume density of the nucleus and in the number of dark cellforms. Arch. Histol. Cytol. 1995, 58, 417-425.
• 15. Mori S.: Light and electron microscopic features and frequencies of the glial cell present in the cerebral cortex of the rat brain. Arch. Histol. Jnp 1972, 34, 231-244.
• 16. Mori S. I., Leblond C. P.: Electron microscopic identification of three classes of oligodendrocytes and a preliminary study of their proliferative activity inthe corpus callosum of young rats. J. Comp. Neurol. 1970, 139, 1-30.
• 17. Murtie J. C., Macklin W. B., Corfas G.: Morphometric analysis of oligodendrocytes in the adult mouse frontal cortex. J. Neurosci. Res. 2007, 85, 2080-2086.
• 18. Muton L. J., Klop E.-M., Broman J., Zhang M., Holstege G.: Lateral cervical nucleus projections to periaqueductal gray matter of cat. J. Comp. Neurol.2004, 471, 434-445.
• 19. Pakkenberg B., Pelvig D., Marner L., Bundgaard M. J., Gundersen H. J. G., Nyengaard J. R., Regeur L.: Aging and the human neocortex. Exp. Gerontol.2003, 38, 95-99.
• 20. Peinado M. A., Quesada A., Pedrosa J., Torres M. I., Martinem M., Esteban F. J., Del Moral M., Hernandez R., Rodrigo J.: Quantitative and ultrastructuralchanges in glia and pericytes in the parietal cortex of the aging rat. MicroscopyRes. Techn. 1998, 43, 34-42.
• 21. Peters A.: The effect of normal aging on myelinated nerve fibres in monkey central nervous system. Frontier Neuroanat. 2009, 3, 1-10.
• 22. Peters A., Sethares C.: Is there remyelination during aging of the primate central nervous system? J. Comp. Neurol. 2003, 460, 238-254.
• 23. Peters A., Sethares C.: Oligodendrocytes, their progenitors and other neuroglial cells in the aging primate cerebral cortex. Cerebrebral Cortex 2004, 14, 995-1007.
• 24. Rees S.: A quantitative electron microscope study of the ageing human cerebral cortex. Acta Neuropathol. 1976, 36, 347-362.
• 25. Richter-Landsberg C.: The cytoskeleton in oligodendrocytes. Microtubule dynamics in health and disease. J. Mol. Neurosci. 2008, 35, 55-63.
• 26. Sato T.: A modified method for lead staining of thin sections. Electron Microsc. 1968, 17, 158-159.
• 27. Sturrock R. R.: Identification of mitotic oligodendrocytes in semithin sections of the developing mouse corpus callosum and hippocampal commissure.J. Anat. 1983, 137, 47-55.
• 28. Uranova N. A., Vostrikov V. M., Vikhreva O. V., Zimina I. S., Kolomeets N. S., Orlovskaya D. D.: The role of oligodendrocyte pathology in schizophrenia.Int. J. Neuropsychopharmacol. 2007, 10, 537-545.
• 29. Uranova N. A., Vikhreva O. V., Rachmanova V. I., Orlovskaya D. D.: Ultrastructural alterations of myelinated fibers and oligodendrocytes in the prefrontalcortex in schizophrenia: a postmortem morphometric study. Schizophr. Res.Treatment 2011, 325789. doi: 10.1155/2011/325789.
• 30. Vaughan D. W., Peters A.: Neuroglial cells in the cerebral cortex of rats from young aldulthood to old age: an electron microscope study. J. Neurocytol. 1974, 3, 404-433.
• 31. Vianna D. M. I., Brandão M. I.: Anatomical connections of the periaqueductal gray: specific neural substratem for different kinds of fear. Braz. J. Med. Biol.Res. 2003, 36, 557-566.
• 32. Vinet J., Lemieux P., Tamburri A., Tiesinga P., Scafidi J., Gallo V., Sík A.: Subclasses of oligodendrocytes populate the mouse hippocampus. Eur. J.Neurosci. 2010, 31, 425-438.
• 33. Watanabe M., Toyama Y., Nishiyama A.: Differentiation of proliferating NG2- positive glial progenitor cells in a remyelinating lesion. J. Neurosci. Res. 2002, 69, 826-836.