Mitochondrial cytochrome b sequences resolve the taxonomy of field mice (Apodemus) in the western Balkan refugium

• Autorzy: Krystufek B. , Luznik M. , Buzan E. V.
• Języki publikacji: EN

Abstrakty

EN

Apodemus sylvaticus stankovici, described from the topographically rough landscape of the western Balkan glacial refugium, was recently proposed as being either a junior synonym of Apodemus flavicollis or a species on its own right. To untangle this taxonomic vagueness, we sequenced complete cytochrome b gene in 28 field mice collected at 12 locations in the mountains of Bosnia and Herzegovina, Montenegro, western Macedonia and northern Greece. Samples yielded 27 new haplotypes which clustered into two distinct groups. One of these clades also included the reference haplotype of A. flavicollis, while another cluster emerged as being identical with the reference sample for A. sylvaticus. As is common in Apodemus, both species retrieved in our analysis were characterized by low levels of intraspecific variation (0.4–0.9%) as opposed to a high level of differentiation between them (8.0–10.0%); therefore, the taxonomic classification of our material was without doubt. We found no evidence regarding the presence of an additional cryptic species in the mountains of the western Balkans. The very similar values of genetic variability in the two species imply their common evolutionary history of a long-term coexistence in the western Balkan refugium.

Słowa kluczowe

EN

mitochondrial cytochrome b; cytochrome b sequence; taxonomy; field mouse; mouse; Apodemus; Balkan Peninsula; Apodemus flavicollis; Apodemus sylvaticus; Apodemus stankovici; cytochrome b gene; intraspecific variation; differentiation; genetic variability

• Wydawca: -
• Czasopismo: Acta Theriologica
• Rocznik: 2012
• Tom: 57
• Numer: 1
• Opis fizyczny: p.1-7,fig.,ref.

Twórcy

autor

Krystufek B.

• Science and Research Centre, University of Primorska, Garibaldijeva 1, SI-6000 Koper, Slovenia

autor

Luznik M.

• Science and Research Centre, University of Primorska, Garibaldijeva 1, SI-6000 Koper, Slovenia

autor

Buzan E. V.

• Science and Research Centre, University of Primorska, Garibaldijeva 1, SI-6000 Koper, Slovenia

Bibliografia

• Anisimova M, Gascuel O (2006) Approximate likelihood ratio test for branches: a fast, accurate and powerful alternative. Syst Biol 55:539–552
• Bego F, Kryštufek B, Paspali G, Rogozi E (2008) Small terrestrial mammals in Albania: annotated list and distribution. Hystrix 19:83–101
• Bellinvia E (2004) A phylogenetic study of the genus Apodemus by sequencing the mitochondrial DNA control region. J Zoolog Syst Evol Res 42:289–297
• Bilton DT, Mirol PM, Mascheretti S, Fredga K, Zima J, Searle JB (1998) Mediterranean Europe as an area of endemism for small mammals rather than a source of northwards post-glacial colonization. Proc R Soc Lond B Biol Sci 265:1219–1226
• Bugarski-Stanojević V, Blagojević J, Anađević T, Jojić V, Vujošević M (2008) Molecular phylogeny and distribution of three Apodemus species (Muridae, Rodentia) in Serbia. J Zoolog Syst Evol Res 46:278–286
• Buzan E, Förster DW, Searle B, Kryštufek B (2010) A new cytochrome b phylogroup of the common vole (Microtus arvalis) endemic to the Balkans and its implications for the evolutionary history of the species. Biol J Linn Soc 100:788–796
• Corbet GB (1978) The mammals of the Palaearctic region: a taxonomic review. British Museum (Natural History), London, pp 1–314
• Dubey S, Michaux J, Brünner H, Hutterer R, Vogel P (2009) False phylogenies on wood mice due to cryptic cytochrome-b pseudogene. Mol Phylogenet Evol 50:633–641
• Đulić B, Mirić Đ (1967) Catalogus Faunae Jugoslaviae IV/4: Mammalia. Academia scientiarum et artium Slovenica, Ljubljana, pp 1–46
• Ellerman JR, Morrison-Scott TCS (1966) Checklist of Palaearctic and Indian mammals 1758 to 1946. 2nd ed. British Museum (Natural History), London, pp 1–810
• Ewing B, Hillier L, Wendl MC, Green P (1998) Base-calling of automated sequencer traces using Phred. I. Accuracy assessment. Genome Res 8:175–185
• Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567
• Griffiths HI, Kryštufek B, Reed JM (eds) (2004) Balkan biodiversity: pattern and process in the European hotspot. Kluwer, Dordrecht, pp 1–357
• Guindon S, Gascuel O (2003) A simple, fast, and accurate algorithm to estimate phylogenies by maximum likelihood. Syst Biol 52:696–704
• Hewitt GM (2000) The genetic legacy of the Quaternary ice ages. Nature 405:907–913
• Hoofer SR, Gaschak S, Dunina-Barkovskaya Y, Makluk J, Meeks HN, Wickliffe JK, Baker RJ (2007) New information for systematics, taxonomy, and phylogeography of the rodent genus Apodemus (Sylvaemus) in Ukraine. J Mammal 88:330–342
• Huelsenbeck JP, Ronquist F (2001) MrBayes: Bayesian inference of phylogeny. Bioinformatics 17:754–755
• Jaarola M, Searle JB (2002) Phylogeography of field voles (Microtus agrestis) in Eurasia inferred from mitochondrial DNA sequences. Mol Ecol 11:2613–2621
• Kryštufek B, Stojanovski L (1996) Apodemus sylvaticus stankovici is a synonym of Apodemus flavicollis. Folia Zool 45:1–7
• Kryštufek B, Buzan EV, Hutchinson WF, Hänfling B (2007) Phylogeography of the rare Balkan endemic Martino’s vole, Dinaromys bogdanovi, reveals strong differentiation within the western Balkan Peninsula. Mol Ecol 16:1221–1232
• Kryštufek B, Bryja J, Bužan EV (2009) Mitochondrial phylogeography of the European ground squirrel, Spermophilus citellus, yields evidence on refugia for steppic taxa in the southern Balkans. Heredity 103:129–135
• Martin Y, Gerlach G, Schlötterer C, Meyer A (2000) Molecular phylogeny of European muroid rodents based on complete cytochrome b sequences. Mol Phylogenet Evol 16:37–47
• Martino V, Martino E (1937) Preliminary note on four new rodents from Korab mountains. Ann Mag Nat Hist 19:514–518, Series 10
• Mezhzherin SV (1987) Genetic differentiation in wood mice, subgenus Sylvimus. Dokl Akad Nauk SSSR 296:1255–1258 [in Russian]
• Michaux JR, Chevret P, Filippucci M-G, Macholan M (2002) Phylogeny of the genus Apodemus with a special emphasis on the subgenus Sylvaemus using the nuclear IRBP gene and two mitochondrial markers: cytochrome b and 12S rRNA. Mol Phylogenet Evol 23:123–136
• Michaux JR, Magnanou E, Paradis E, Nieberding C, Libois R (2003) Mitochondrial phylogeography of the woodmouse (Apodemus sylvaticus) in the western Palearctic region. Mol Ecol 12:685–697
• Michaux JR, Libois R, Paradis E, Filippucci M-G (2004) Phylogeographic history of the yellow-necked fieldmouse (Apodemus flavicollis) in Europe and in the Near and Middle East. Mol Phylogenet Evol 32:788–798
• Miller GS (1912) Catalogue of the mammals of Western Europe (Europe exclusive of Russia) in the collection of the British Museum. British Museum, London, pp 1–1019
• Musser GG, Carleton MD (2005) Superfamily Muroidea. In: Wilson DE, Reeder DAM (eds) Mammal species of the world: a taxonomic and geographic reference. 3rd ed., vol. 2. John Hopkins University Press, Baltimore, pp 894–1531
• Nylander JAA (2004) Mrmodeltest, Version 2.2. Uppsala University, Department of Systematic Zoology, Uppsala
• Petrov BM (1992) Mammals of Yugoslavia. Insectivores and rodents. Natural History Museum, Belgrade, pp 1–186, Suppl. 37
• Petrov BM (1994) Apodemus stankovici V. & E. Martino: new ideas on the taxonomic status of some rodent species in the former and actual Yugoslavia. Bull Nat Hist Mus 48:183–188, Series B
• Posada D, Crandall KA (1998) Modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818
• Reutter BA, Petit E, Brünner H, Vogel P (2003) Cytochrome b haplotype divergences in Western European Apodemus. Mammalian biology 68:153–164
• Ronquist F, Huelsenbeck JP (2003) MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574
• Rovatsos MT, Mitsainas GP, Tryfonopoulos GA, Stamatopoulos C, Giagia-Athanasopoulou EB (2008) A chromosomal study on Greek populations of the genus Apodemus (Rodentia, Murinae) reveals new data on B chromosome distribution. Acta Theriol 53:157–167
• Serizawa K, Suzuki H, Tsuchiya K (2000) A phylogenetic view on species radiation in Apodemus inferred from variation of nuclear and mitochondrial genes. Biochem Genet 38:27–41
• Shimodaira H, Hasegawa M (1999) Multiple comparisons of log-likelihoods with applications to phylogenetic inference. Mol Biol Evol 16:1114–1116
• Spitzenberger F (ed) (2001) Die Säugetierfauna Österreichs. Bundesministerium für Land- und Forstwirtschaft, Umwelt und Wassertwirtschaft, Wien: 1–895
• Storch G, Lütt O (1989) Artstatus der Alpenwaldmaus, Apodemus alpicola Heinrich, 1952. Zeitschrift für Säugetierkunde 54:337–346
• Suzuki H, Filippucci MG, Chelomina GN, Sato JJ, Serizawa K, Nevo E (2008) A biogeographic view of Apodemus in Asia and Europe inferred from nuclear and mitochondrial gene sequences. Biochem Genet 46:329–346
• Swofford DL (2002) PAUP*. Phylogenetic analysis using parsimony (*and other methods), version 4.0b10. Sinauer Associates, Sunderland
• Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
• Vorontsov NN, Mezhzherin SV, Boyeskorov GG, Lyapunova EA (1989) Genetic differentiation in sibling species of wood mice (Apodemus) of the Caucasus and their diagnostics. Dokl Akad Nauk SSSR 309:1234–1238 [in Russian]
• Vorontsov NN, Boyeskorov GG, Mezhzherin SV, Lyapunova EA, Kandaurov AS (1992) Systematics of the Caucasian wood mice of the subgenus Sylavemus (Mammalia, Rodentia, Apodemus). Zool Zh 71:119–131