PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 36 | 06 |

Tytuł artykułu

Antioxidant activity evaluation of essential oil and RAPD analysis of in vitro regenerated Blumea mollis (D.Don) Merr.

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Essential oil obtained from the leaves of Blumea mollis (D. Don) Merr. was analyzed by gas chromatography and gas chromatography coupled with mass spectroscopy (GC and GC–MS). Bicyclic sesquiterpene, β-caryophyllene, was identified as a major compound which accounted for 24.54 %. Antioxidant activity of oil was significantly higher than that of methanol extract of callus. Murashige and Skoog (MS) medium supplemented with 4.4 μM BA showed multiple shoot induction after 8 weeks of culture. 4.6 μM Kin showed in vitro flowering and direct organogenesis was observed from the leaf explants on medium containing 4.4 μM BA and 5.4 μM NAA. Rooted plantlets developed on half strength MS medium fortified with 2.1 μM IBA were hardened and percentage survival was recorded up to 70. RAPD analysis revealed a little genetic variation in micropropagated plants.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

36

Numer

06

Opis fizyczny

p.1593-1598,fig.,ref.

Twórcy

  • Laboratory of Plant Tissue Culture and Molecular Bioprospection, PG and Research Department of Biotechnology, Kongunadu Arts and Science College, GN Mills, 641029 Coimbatore, Tamil Nadu, India
  • Laboratory of Plant Tissue Culture and Molecular Bioprospection, PG and Research Department of Biotechnology, Kongunadu Arts and Science College, GN Mills, 641029 Coimbatore, Tamil Nadu, India

Bibliografia

  • Adams RP (1995) Identification of essential oil components by gas chromatography and mass spectrometry, 3rd edn. Allured Publication Corporation, USA
  • Bhuiyan MNI, Chowdhury JU, Begum J (2009) Chemical components in volatile oil from Blumea balsamifera (L.) DC. Bangladesh J Bot 38:107–109
  • Blois MS (1958) Antioxidant determination by use of a stable free radical. Nature 181:1199–1200
  • Calleja MA, Vieites JM, Montera-Meterdez T, Torres MI, Faus MJ, Gil A, Suarez A (2013) The antioxidant effect of β-caryophyllene protects rat liver from carbon tetrachloride-induced fibrosis by inhibiting hepatic stellate cell activation. Br J Nutr 109:394–401
  • Choi B, Kang S, Ha Y, Park G, Ackman RG (2002) Conjugated linoleic acid as a supplemental nutrient for common carp (Cyprinus carpio). Food Sci Biotechnol 11:457–461
  • Chu SS, Du SS, Liu ZL (2013) Fumigant compounds from the essential oil of chinese Blumea balsamifera leaves against the maize weevil (Sitophilus zeamais). J Chem doi:10.1155/2013/289874
  • De Souza AV, Pinto JEBP, Bertolucci SKV, Correa RM (2007) In vitro propagation of Lychnophora pinaster (Asteraceae): a threatened endemic medicinal plant. Hort Sci 42:1665–1669
  • Dinis TCP, Madeira VMC, Almeida MLM (1994) Action of phenolic derivates (acet aminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 315:161–169
  • Do TL (2005) Blumea lanceolaria (Roxb.) Duce. In: Dang TV, Hoang TQ, Do TB (eds) Medicinal plants and drugs from Vietnam, Medical Publishing House, Hanoi
  • Halliwell B, Gutteridge JMC, Aruoma OI (1987) The deoxyribose method: a simple ‘‘test-tube’’ assay for determination of rate constants for reactions hydroxyl radical. Anal Biochem 165:215–219
  • Huang L, Lin C, Li A, Wei B, Teng J, Li L (2010) Procoagulant activity of phenolic acids isolated from Blumea riparia. Nat Prod Commun 5:1263–1266
  • Husain MK, Anis M (2006) Rapid in vitro propagation of Eclipta alba (L.) Hassk. through high frequency axillary shoot proliferation. Acta Physiol Plant 28:325–330
  • Jain SC, Jain R, Renu Singh R (2010) Micropropagation of Verbesina encelioides: an invasive weed. Ind J Biotechnol 9:333–335
  • Lin CW, Yu CW, Wu SC, Yih KH (2009) DPPH free radical scavenging activity, total phenolic contents and chemical composition analysis of forty-two kinds of essential oils. J Food Drug Anal 17:386–395
  • Mahesh A, Thangadurai D, Melchia G (2012) Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze. Biol Res 45:131–136
  • Minerva G, Kumar S (2013) Micropropagation of Gerbera (Gerbera jamesonii Bolus). Methods Mol Biol doi:10.1007/978-1-62703-074-8_24
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
  • Norikura T, Kojima Yuasa A, Shimizu M, Huang X, Xu S, Kametani S, Rho S, Kennedy DO, Matsui-Yuasa I (2008) Mechanism of growth inhibitory effect of Blumea balsamifera extract in hepatocellular carcinoma. Biosci Biotechnol Biochem 72:1183–1189
  • Okunade AL (2002) Ageratum conyzoides L. (Asteraceae). Fitoterapia 73:1–16
  • Padmalatha K, Prasad MNV (2006) Optimization of DNA isolation and PCR protocol for RAPD analysis of selected medicinal and aromatic plants of conservation concern from peninsular India. Afr J Biotechnol 5:230–234
  • Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York
  • Senthilkumar A, Kannathasan K, Venkatesalu V (2008) Chemical constituents and larvicidal property of the essential oil of Blumea mollis (D. Don) Merr. against Culex quinquefasciatus. Parasitol Res 103:959–962
  • Senthilkumar A, Kannathasan K, Venkatesalu V (2009) Antibacterial activity of the leaf essential oil of Blumea mollis (D. Don) Merr. World J Microbiol Biotechnol 25:1297–1300
  • Singh B, Sharma S, Rani G, Virk GG, Zaidi AA, Nagpal A (2006) In vitro flowering in embryogenic cultures of Kinnow mandarin (Citrus nobilis Lour C. deliciosa Tenora). Afr J Biotechnol 5:1470–1474
  • Singh SK, Raj MK, Asthana P, Sahoo L (2009) An improved micropropagation of Spilanthes acmella L. through transverse thin cell layer culture. Acta Physiol Plant 31:693–698
  • Zhu L, Tian Y (2011) Chemical composition and larvicidal effects of essential oil of Blumea martiniana against Anopheles anthropophagus. Asian Pac J Trop Bio Med 4:371–374

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-89656efb-3428-4d85-8771-f262daa1e89f
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.