PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2012 | 34 | 5 |

Tytuł artykułu

Osmotic stress induced-capsaicin production in suspension cultures of Capsicum chinense Jacq.cv. Naga King Chili

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The influence of osmotic stress on capsaicin production was investigated in cell suspension cultures of Capsicum chinense Jacq.cv. Naga King Chili, a chili species native to Northeastern India. The sterilized seeds were germinated in Murashige and Skoog medium. Two-weekold hypocotyls were excised from in vitro germinated seedlings and implanted in MS medium containing 2, 4-dichlorophenoxyacetic acid (2 mg/l), and Kinetin (0.5 mg/l) for callus induction. Capsaicin production in the suspension cultures was significantly affected using sucrose, mannitol, and NaCl in the medium. Stoichiometric analysis with different combinations of sucrose and nonsugar osmotic agent (NaCl) showed that osmotic stress was an important factor for enhancing capsaicin production in cell suspension cultures of C. chinense. The capsaicin content of 1,644.1 µg g⁻¹ f.wt was recorded on day 15 in cultures grown in MS medium containing 87.64 mM sucrose in combination with 40 mM NaCl. However, osmotic stress treatment at 160 mM NaCl with sucrose resulted in lowering capsaicin accumulation and separation of cell wall from their cytoplasm, under microscopic observation.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

34

Numer

5

Opis fizyczny

p.2039-2044,fig.,ref.

Twórcy

autor
  • Plant Biotechnology Laboratory, Centre for Advanced Studies in Botany, North-Eastern Hill University, 793022 Shillong, India
autor
  • Plant Biotechnology Laboratory, Centre for Advanced Studies in Botany, North-Eastern Hill University, 793022 Shillong, India
autor
  • Plant Biotechnology Laboratory, Centre for Advanced Studies in Botany, North-Eastern Hill University, 793022 Shillong, India

Bibliografia

  • Bhagowati RR, Changkija S (2001) Genetic variability and traditional practices in Naga King Chili landraces of Nagaland. Asian Agri-History 13:171–180
  • Borowitzka LJ (1981) Solute accumulation and regulation of cell water activity. In: Paleg LG, Aspinall D (eds) The physiology and biochemistry of drought resistance in plants. Academic press, Sydney, pp 97–127
  • Do BC, Cormier F (1990) Accumulation of anthocyanins enhanced by a high osmotic potential in grape (Vis vinifera L.) cell suspensions. Plant cell Rep 9:143–146
  • Fusco BM, Giacovazzo M (1997) Peppers and pain. The promise of capsaicin. Drugs 53:909–914
  • Guinness Book of World Records (2006) Hottest spice. http://www.guinnessworldrecords.com
  • Gutierrez-Carbajal MG, Monforte-Gonzalez M, Miranda-Ham MdeL, Godoy-Hernandez G, Vazquez-Flota F (2010) Induction of capsaicinoid synthesis in Capsicum chinense cell cultures by salicylic acid or methyl jasmonate. Biol Plant 54:430–434
  • Kehie M, Kumaria S, Tandon P (2011) In vitro plantlet regeneration from nodal segments and shoot tips of Capsicum chinense Jacq. Cv. Naga King Chili. 3 Biotech 2:31–35
  • Kim SI, Choi HK, Kim JH (2001) Effect of osmotic pressure on paclitaxel production in suspension cell cultures of Taxus chinensis. Enzym Microb Tech 28:202–209
  • Liu C, Cheng X (2008) Enhancement of phenylethanoid glycosides biosynthesis in cell cultures of Cistanche deserticola by osmotic stress. Plant Cell Rep 27:357–362
  • Mazzone SB, Geraghty DP (1999) Respiratory action of capsaicin microinjected into the nucleus of the solitary tract: involvement of vanilloid and tachykinins receptors. Br J Pharmacol 127:473–481
  • Meghvansi MK, Siddiqui S, Haneef Khan M, Gupta VK, Vairale MG, Gogoi HK, Singh L (2010) Naga chilli: a potential source of capsaicinoids with broad-spectrum ethnopharmacological applications. J. Ethnopharmacol 132:1–14
  • Min JK, Han KY, Kim EC, Kim YM, Lee KR, Kim OH, Kim KW, Gho YS, Kwon YG (2004) Capsaicin inhibits in vitro and in vivo angiogenesis. Cancer Res 64:644–651
  • Mukherjee SK, Sabapathi RB, Gupta N (1991) Low sugars and osmotic requirements for shoot regeneration from leaf pieces of Solanum melongena L. Plant Cell Tissue Org Cult 25:13–16
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
  • Nunez-Palenius HG, Ochoa-Alejo N (2005) Effect of phenylalanine and phenylpropanoids on the accumulation of capsaicinoids and lignins in cell cultures of chili peppers (Capsicum annum L.). In Vitro Dev Biol Plant 41:801–805
  • Ochoa-Alejo N, Salgado-Garciglia R (1992) Phenylalanine ammonialyase activity and capsaicin-precursor compounds in p-fluorophenylalanine-resistant and sensitive variant cells of chili pepper (Capsicum annuum). Physiol Plant 85:173–179
  • Perucka I, Oleszek W (2000) Extraction and determination of capsaicinoids in fruit of hot pepper Capsicum annuum L. by spectrophotometry and high-performance liquid chromatography. Food Chem 71:287–291
  • Rao SR, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20:101–153
  • Ravishankar GA, Sarma KS, Venkataraman LV, Kadyan AK (1988) Effect of nutritional stress on capsaicin production in immobilized cell cultures of Capsicum annuum. Curr Sci 57:381–383
  • Salgado-Garciglia R, Ochoa-Alejo N (1990) Increased capsaicin content in PFP resistant cells of chili pepper (Capsicum annuum L.). Plant Cell Rep 8:617–620
  • ShohaelAM,HakrabartiD,AliMB,YuKW,Hahn EJ, LeeHL, PaekKY (2006) Enhancement of eleutherococcuc sessiliflorus in response to sucrose-induced osmotic stress. Process Biochem 41:512–518
  • Suzuki JI, Tausig F, Morse RE (1957) Some observation on red pepper. 1. A new method for determination of pungency in red pepper. Food Technol 11:100–104
  • Suzuki T, Kawada T, Iwai K (1981) Biosynthesis of acyl moieties of capsaicin and its analogues from valine and leucine in Capsicum fruits. Plant Cell Physiol 22:23–32
  • Tholakalabavi A, Zwiazek JJ, Thorpe TA (1994) Effect of mannitol and glucose induced osmotic stress on growth, water relations, and solute composition of cell suspension cultures of poplar (Populus deltoids var. occidentalis) in relation to anthocyanin accumulation. In Vitro Cell Dev Biol 30:164–170

Uwagi

Rekord w opracowaniu

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-89575ba4-2f77-43e5-be60-8044c727e913
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.