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2018 | 17 | 1 |

Tytuł artykułu

Evaluation of antioxidant activity of in vitro propagated medicinal Ceratophyllum demersum L. extracts

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Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Ceratophyllum demersum L. is a medical plant of the family Ceratophyllaceae that has been traditionally used for the treatment of diseases such as ulcer, diarrhoea, wounds and fever since ancient times. This study presents antioxidant activities of water and methanol extracts of in vitro propagated C. demersum. Shoot tip explants of C. demersum for in vitro plant regeneration were cultured on liquid Murashige and Skoog (MS) medium containing 0.25–1.25 mg/L 6-Benzylaminopurine (BAP) and 0.10 mg/L Thidiazuron (TDZ) combinations for eight weeks. Maximum number of plantlets per explant (110.67) was obtained on MS medium supplemented with 0.75 mg/L BAP + 0.10 mg/L TDZ. In order to determine antioxidant activities of C. demersum, antioxidant contents (β-carotene, flavonoid, lycopene and total phenols), metal chelating and reducing power capacities of methanol and water extracts obtained from C. demersum were investigated. It was detected that water extract was more effective in all activities. Total phenols (76.55 μg/mg) in water extract were measured as the highest antioxidant component. Based on the water extracts data, as IC50 value for chelating on ferrous ions was 9.24 mg/mL, EC50 value for reducing power activity was 8.23 mg/mL. It was also determined that the metal chelating and reducing power activities of the both extracts are increased depending on the concentration. Therefore, all these data suggest that both tested extracts of in vitro propagated C. demersum would exert beneficial effects by virtue of their antioxidant properties and may be utilized as a promising source of therapeutics.

Wydawca

-

Rocznik

Tom

17

Numer

1

Opis fizyczny

p.23-33,fig.,ref.

Twórcy

autor
  • Department of Biology, Kamil Ozdag Faculty of Science, Karamanoglu Mehmetbey University, Karaman, Turkey
autor
  • Department of Biology, Kamil Ozdag Faculty of Science, Karamanoglu Mehmetbey University, Karaman, Turkey

Bibliografia

  • Abourashed, E. (2013). Bioavailability of plant-derived antioxidants. Antioxidants, 2, 309–325.
  • Arber, A. (2010). Water plants. A study of aquatic angiosperms. Cambridge University Press, New York. Ashraf, M.F., Aziz, M.A., Kemat, N., Ismail, I. (2014). Effect of cytokinin types, concentrations and their interactions on in vitro shoot regeneration of Chlorophytum borivilianum Sant. & Fernandez. Electron. J. Biotechnol., 17, 275–279.
  • Augustyniak, A., Bartosz, G., Cipak, A., Duburs, G., Horáková, L., Luczaj, W., Majekova, M., Odysseos, A.D., Rackova, L., Skrzydlewska, E., Stefek, M., Strosová, M., Tirzitis, G., Venskutonis, P.R., Viskupicova, J., Vraka, P.S., Zarković, N. (2010). Natural and synthetic antioxidants: An updated overview. Free Radic. Res., 44, 1216–1262.
  • Baksha, R., Jahan, M.A.A., Khatun, R., Munshi, J.L. (2005). Micropropagation of Aloe barbadensis Mill. through in vitro culture of shoot tip explants. Plant Tissue Cult. Biotechnol., 15, 121–126.
  • Bankova, V., Ivanova, P., Christov, R., Popov, S., Dimitrova-Konaklieva, S. (1995). Secondary metabolites of Ceratophyllum demersum. Hydrobiologia, 316, 59–61.
  • Beddou, F., Bekhechi, C., Ksouri, R., Chabane Sari, D., Atik Bekkara, F. (2015). Potential assessment of Rumex vesicarius L. as a source of natural antioxidants and bioactive compounds. J. Food Sci. Technol., 52, 3549–3560.
  • Carocho, M., Ferreira, I.C.F.R. (2013). A review on antioxidants, prooxidants and related controversy: Natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food Chem. Toxicol., 51, 15–25.
  • Carvalho Costa, D., Costa, H., Gonçalves Albuquerque, T., Ramos, F., Castilho, M., Sanches-Silva, A., Jorge, R.
  • (2015). Advances in phenolic compounds analysis of aromatic plants and their potential applications. Trends Food Sci. Technol., 45, 336–354.
  • Chen, L.J., Hu, TW., Huang, L.C. (1995). A protocol toward multiplication of the medicinal tree, Eucommia ulmoides Oliver. In Vitro Cell. Dev. Biol. – Plant, 31, 193–198.
  • Devasagayam, T.P.A., Tilak, J.C., Boloor, K.K., Sane, K.S., Ghaskadbi, S.S., Lele, R.D. (2004). Free radicals and antioxidants in human health: Current status and future prospects. J. Assoc. Phys. India, 52, 794–804.
  • Dogan, M. (2013). In vitro propagation of coontail (Ceratophyllum demersum L.). Dissertation, Karamanoğlu Mehmetbey University. Emsen, B., Aslan, A., Togar, B., Turkez, H. (2016). In vitro antitumor activities of the lichen compounds olivetoric, physodic and psoromic acid in rat neuron and glioblastoma cells. Pharm. Biol., 54, 1748–1762.
  • Emsen, B., Turkez, H., Togar, B., Aslan, A. (2017). Evaluation of antioxidant and cytotoxic effects of olivetoric and physodic acid in cultured human amnion fibroblasts. Hum. Exp. Toxicol., 36, 376–385.
  • Eng, W.H., Aziz, M.A., Sinniah, U.R. (2014). Shoot regeneration of limau purut (Citrus hystrix) using shoot tip: Assessment of calcium gluconate and silver nitrate in overcoming premature leaf senescence. Pak. J. Bot., 46, 1453–1458.
  • Fareed, M.F., Haroon, A.M., Rabeh, S.A. (2008). Antimi- crobial activity of some Macrophytes from Lake Manzalah (Egypt). Pak. J. Biol. Sci., 11, 2454–2463.
  • Gnanaraj, W.E., Marimuthu, J., Subramanian, K.M., Nallyan, S. (2011). Micropropagation of Alternanthera sessilis (L.) using shoot tip and nodal segments. Iran. J. Biotechnol., 9, 206–212.
  • Gulcin, I., Uguz, M.T., Oktay, M., Beydemir, S., Kufrevioglu, O.I. (2004). Evaluation of the antioxidant and antimicrobial activities of clary sage (Salvia sclarea L.). Turkish J. Agric. For., 28, 25–33.
  • Gupta, S., Sahu, P.K., Sen, D.L., Pandey, P. (2014). Invitro propagation of Aloe vera (L.) Burm. F. Br. Biotechnol. J., 4, 806–816.
  • Heimler, D., Isolani, L., Vignolini, P., Tombelli, S., Romani, A. (2007). Polyphenol content and antioxidative activity in some species of freshly consumed salads. J. Agric. Food Chem., 55, 1724–1729.
  • Hinneburg, I., Damien Dorman, H.J., Hiltunen, R. (2006). Antioxidant activities of extracts from selected culinary herbs and spices. Food Chem., 97, 122–129.
  • Hofer, T., Jørgensen, T.O., Olsen, R.L. (2014). Compari- son of food antioxidants and iron chelators in two cellular free radical assays: Strong protection by Luteolin. J. Agric. Food Chem., 62, 8402–8410.
  • Hussey, G. (1982). In vitro propagation of monocotyledo- nous bulbs and corms. In: Proceedings of the 5th International Plant Tissue and Cell Culture, July 11–16. Tokyo, 677–680.
  • Jo, U.A., Murthy, H.N., Hahn, E.J., Paek, K.Y. (2008). Micropropagation of Alocasia amazonica using semisolid and liquid cultures. In Vitro Cell. Dev. Biol. – Plant, 44, 26–32.
  • Karamac, M., Amarowicz, R., Weidner, S., Abe, S., Shahidi, F. (2000). Antioxidant activity of rye caryopses and embryos extracts. Czech J. Food Sci., 20, 209–214.
  • Keskinkan, O., Goksu, M.Z.L., Basibuyuk, M., Forster, C.F. (2004). Heavy metal adsorption properties of a submerged aquatic plant (Ceratophyllum demersum). Bioresour. Technol., 92, 197–200.
  • Lange, D. (2006). International trade in medicinal and aromatic plants. Actors, volumes and commodities. Med. Aromat. Plants, 155–170.
  • Liu, Y., Du, Y., Wang, J., Zha, X., Zhang, J. (2014). Structural analysis and antioxidant activities of polysaccharide isolated from Jinqian mushroom. Int. J. Biol. Macromol., 64, 63–68.
  • Lu, Y., Yeap Foo, L. (2001). Antioxidant activities of polyphenols from sage (Salvia officinalis). Food Chem., 75, 197–202.
  • Makowczyńska, J., Sliwinska, E., Kalemba, D., Piątczak, E., Wysokińska, H. (2016). In vitro propagation, DNA content and essential oil composition of Teucrium scorodonia L. ssp. scorodonia. Plant Cell. Tissue Organ Cult., 127, 1–13.
  • McKay, D.L., Chen, C.Y.O., Zampariello, C.A., Blumberg, J.B. (2015). Flavonoids and phenolic acids from cranberry juice are bioavailable and bioactive in healthy older adults. Food Chem., 168, 233–240.
  • Meir, S., Kanner, J., Akiri, B., Philosoph-Hadas, S. (1995). Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J. Agric. Food Chem., 43(7), 1813– 1819.
  • Mena, S., Ortega, A., Estrela, J.M. (2009). Oxidative stress in environmental-induced carcinogenesis. Mutat. Res., 674, 36–44.
  • Muhammad, A., Rashid, H., Hussain, I. (2007). Proliferation-rate effects of BAP and kinetin on banana (Musa spp. AAA Group) “Basrai.” HortScience, 42, 1253–1255.
  • Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15, 473–497.
  • Nikoo, M., Benjakul, S. (2015). Potential application of seafood-derived peptides as bifunctional ingredients, antioxidant-cryoprotectant: A review. J. Funct. Foods, 19, 753–764.
  • Oyetayo, V.O., Dong, C.H., Yao, Y.J. (2009). Antioxidant and antimicrobial properties of aqueous extract from Dictyophora indusiata. Open Mycol. J., 3, 20–26.
  • Ozkan, G., Simsek, B., Kuleasan, H. (2007). Antioxidant activities of Satureja cilicia essential oil in butter and in vitro. J. Food Eng., 79, 1391–1396.
  • Öztürk, M., Khawar, K.M., Atar, H.H., Sancak, C., Özcan, S. (2004). In vitro micropropagation of the aquarium plant Ludwigia repens. Asia Pacific J. Mol. Biol. Biotechnol., 12, 21–25.
  • Pal, J., Ganguly, S., Tahsin, K.S., Acharya, K. (2010). In vitro free radical scavenging activity of wild edible mushroom, Pleurotus squarrosulus (Mont.) Singer. Indian J. Exp. Biol., 48, 1210–1218.
  • Pełechaty, M., Pronin, E., Pukacz, A. (2004). Charophyte occurrence in Ceratophyllum demersum stands. Hydrobiologia, 737, 111–120.
  • Pokorný, J. (2007). Are natural antioxidants better – and safer – than synthetic antioxidants? Eur. J. Lipid Sci. Technol., 109, 629–642.
  • Qiming, X., Haidong, C., Huixian, Z., Daqiang, Y. (2006). Chemical composition of essential oils of two submerged macrophytes, Ceratophyllum demersum L. and Vallisne- ria spiralis L. Flavour Fragr. J., 21, 524–526.
  • Raja, H.D., Arockiasamy, D.I. (2008). In vitro propagation of Mentha viridis L. from nodal and shoot tip explants. Plant Tissue Cult. Biotechnol., 18, 1–6.
  • Sarwar Alam, M., Kaur, G., Jabbar, Z., Javed, K., Athar, M. (2007). Eruca sativa seeds possess antioxidant activity and exert a protective effect on mercuric chloride induced renal toxicity. Food Chem. Toxicol., 45, 910–920.
  • Sharma, N., Satsangi, R., Pandey, R., Devi, S.V. (2007). In vitro clonal propagation and medium term conservation of Brahmi [Bacopa monnieri (L) Wettst]. J. Plant Biochem. Biotechnol., 16, 139–143.
  • Soumia, K., Tahar, D., Lynda, L., Saida, B., Chabane, C., Hafidha, M. (2014). Antioxidant and antimicrobial activities of selected medicinal plants from Algeria. J. Coast. Life Med., 2, 478–483.
  • Sudha, G., Janardhanan, A., Moorthy, A., Chinnasamy, M., Gunasekaran, S., Thimmaraju, A., Gopalan, J. (2016). Comparative study on the antioxidant activity of methanolic and aqueous extracts from the fruiting bodies of an edible mushroom Pleurotus djamor. Food Sci. Biotechnol., 25, 371–377.
  • Svobodova, B., Barros, L., Calhelha, R.C., Heleno, S., Alves, M.J., Walcott, S., Bittova, M., Kuban, V., Ferreira, I.C.F.R. (2017). Bioactive properties and phenolic profile of Momordica charantia L. medicinal plant growing wild in Trinidad and Tobago. Ind. Crops Prod., 95, 365–373.
  • Taga, M.S., Miller, E.E., Pratt, D.E. (1984). Chia seeds as a source of natural lipid antioxidants. J. Am. Oil Chem. Soc., 61, 928–931.
  • Taranhalli, A.D., Kadam, A.M., Karale, S.S., Warke, Y.B. (2011). Evaluation of antidiarrhoeal and wound healing potentials of Ceratophyllum demeresum Linn. whole plant in rats. Lat. Am. J. Pharm., 30, 297–303.
  • Valko, M., Leibfritz, D., Moncol, J., Cronin, M.T., Mazur, M., Telser, J. (2007). Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol., 39, 44–84.
  • Vikram, D.S., Rivera, B.K., Kuppusamy, P. (2010). Free radicals and antioxidant protocols. Free Radicals Antioxid. Protoc., 610, 3–27.
  • Wan, X.L., Niu, Y., Zheng, X.C., Huang, Q., Su, W.P., Zhang, J.F., Zhang, L.L., Wang, T. (2016). Antioxidant capacities of Artemisia annua L. leaves and enzymatically treated Artemisia annua L. in vitro and in broilers. Anim. Feed Sci. Technol., 221, 27–34.
  • Xiao-Li, L., Ying, Q., Xian-Min, Z., Bo-Lin, M., MingHua, Q. (2007). Chemical constituents from Ceratophyllum demersum (Ceratophyllaceae). Acta Bot. Yunnan., 29, 263–264.

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Bibliografia

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