New PCR test for detection on Candida glabrata based on the molecular target chosen by RAPD technique

• Autorzy: Olchawa A. , Krawczyk B. , Brillowska-Dabrowska A.
• Języki publikacji: EN

Abstrakty

EN

Rapid, reliable diagnosis is a necessary condition for the successful treatment of infections. Such diagnostic assays are continually being developed. The paper presents a method for selecting the molecular target for PCR-based diagnostics based on the comparison of RAPD patterns. A sequence encoding Candida glabrata CBS138 hypothetical protein was selected. The limit of detection for PCR and real-time PCR reactions with DNA extracted from blood samples spiked with Candida glabrata was estimated at 1 CFU/ml. The application of the assays developed in this study would thus seem to be promising as a complementary method in the diagnostics of C. glabrata infections.

Słowa kluczowe

EN

polymerase chain reaction; new test; detection; Candida glabrata; molecular target; real-time polymerase chain reaction; random amplified polymorphic DNA technique

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2013
• Tom: 62
• Numer: 1
• Opis fizyczny: p.81-84,fig.,ref.

Twórcy

autor

Olchawa A.

• Department of Microbiology, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdansk, Poland

autor

Krawczyk B.

• Department of Microbiology, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdansk, Poland

autor

Brillowska-Dabrowska A.

• Department of Microbiology, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdansk, Poland

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