PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2016 | 62 | 3 |

Tytuł artykułu

Subtype analysis of Blastocystis sp. isolates from asymptomatic individuals in an urban community in the Philippines

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Blastocystis sp. is a commonly reported enteric protistan parasite in faecal specimens with a worldwide distribution afflicting both humans and a wide range of animals. The aim of this study is to characterize the subtypes (STs) of Blastocystis sp. isolates from asymptomatic individuals in an urban community in Pateros, Metro Manila, Philippines. The 600-bp small subunit ribosomal RNA (SSU rRNA) barcoding region of Blastocystis sp. isolates was amplified and sequenced using the primers RD5 and BhRDr. Subtypes were identified by uploading the sequences onto the Basic Local Alignment and Search Tool (BLAST) websites, the Blastocystis Subtype (18S) and Sequence Typing (MLST) Database and by construction of a phylogenetic tree. Twenty-nine (29) out of 35 individuals were detected positive for Blastocystis sp. ST3 is the most common among the three STs detected (65.5%), followed by ST1 (31.0%) and ST4 (3.44%). This study showed that DNA barcoding can serve as a helpful tool to investigate the diversity of Blastocystis sp. in the Philippines.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

62

Numer

3

Opis fizyczny

p.193–200,fig.,ref.

Twórcy

autor
  • Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City 1101, Philippines
  • Molecular Protozoology Laboratory, Natural Sciences Research Institute, University of the Philippines, Diliman, Quezon City 1101, Philippines
  • Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City 1101, Philippines
  • Department of Parasitology, College of Public Health, University of the Philippines, Ermita, Manila 1000, Philippines
autor
  • Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City 1101, Philippines
  • Molecular Protozoology Laboratory, Natural Sciences Research Institute, University of the Philippines, Diliman, Quezon City 1101, Philippines
autor
  • Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City 1101, Philippines
  • Molecular Protozoology Laboratory, Natural Sciences Research Institute, University of the Philippines, Diliman, Quezon City 1101, Philippines

Bibliografia

  • [1] Stenzel D., Boreham P. 1996. Blastocystis hominis revisited. Clinical Microbiology Reviews 9: 563-584.
  • [2] Tan K.S. 2008. New insights on classification, identification, and clinical relevance of Blastocystis spp. Clinical Microbiology Reviews 21: 639-665.
  • [3] Stensvold C.R. 2013. Blastocystis: genetic diversity and molecular methods for diagnosis and epidemiology. Tropical Parasitology 3: 26-34.
  • [4] El Safadi D., Gaayeb L., Meloni D., Cian A., Poirier P., Wawrzyniak I., Delbac F., Dabboussi F., Delhaes L., Seck M. 2014. Children of Senegal River Basin show the highest prevalence of Blastocystis sp. ever observed worldwide. BMC Infectious Diseases 14: 164. doi:10.1186/1471-2334-14-164.
  • [5] Meloni D., Sanciu G., Poirier P., El Alaoui H., Chabe M., Delhaes L., Dei-Cas E., Delbac F., Luigi Fiori P., Di Cave D., Viscogliosi E. 2011. Molecular subtyping of Blastocystis sp. isolates from symptomatic patients in Italy. Parasitology Research 109: 613-619.
  • [6] Rivera W.L. 2008. Phylogenetic analysis of Blastocystis isolates from animal and human hosts in the Philippines. Veterinary Parasitology 156: 178-182.
  • [7] Yoshikawa H., Wu Z., Pandey K., Pandey B.D., Sherchand J.B., Yanagi T., Kanbara H. 2009. Molecular characterization of Blastocystis isolates from children and rhesus monkeys in Kathmandu, Nepal. Veterinary Parasitology 160: 295-300.
  • [8] Noël C., Peyronnet C., Gerbod D., Edgcomb V.P., Delgado-Viscogliosi P., Sogin M.L., Capron M., Viscogliosi E., Zenner L. 2003. Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequences. Molecular and Biochemical Parasitology 126: 119-123.
  • [9] Suresh K., Smith H., Tan T. 2005. Viable Blastocystis cysts in Scottish and Malaysian sewage samples. Applied and Environmental Microbiology 71: 5619-5620.
  • [10] Alfellani M.A., Taner-Mulla D., Jacob A.S., Imeede C.A., Yoshikawa H., Stensvold C.R., Clark C.G. 2013. Genetic diversity of Blastocystis in livestock and zoo animals. Protist 164: 497-509.
  • [11] Scicluna S.M., Tawari B., Clark C.G. 2006. DNA barcoding of Blastocystis. Protist 157: 77-85.
  • [12] Yakoob J., Jafri W., Jafri N., Khan R., Islam M., Beg M.A., Zaman V. 2004. Irritable bowel syndrome: in search of an etiology: role of Blastocystis hominis. American Journal of Tropical Medicine and Hygiene 70: 383-385.
  • [13] Andiran N., Acikgoz Z.C., Turkay S., Andiran F. 2006. Blastocystis hominis – an emerging and imitating cause of acute abdomen in children. Journal of Pediatric Surgery 41: 1489-1491.
  • [14] Al-Fellani M.A., Khan A.H., Al-Gazoui R.M., Zaid M.K., Al-Ferjani M.A. 2007. Prevalence and clinical features of Blastocystis hominis infection among patients in Sebha, Libya. Sultan Qaboos University Medical Journal 7: 35-40.
  • [15] Jerez Puebla L., Millán I., Núñez Fernández F. 2014. Frequency of blastocystosis and its association with clinical symptoms in 2 years of surveillance at „Pedro Kourí” Institute. Clinical Microbiology 3: 178. doi:10.4172/2327-5073.1000178.
  • [16] Taşova Y., Sahin B., Koltaş S., Paydaş S. 2000. Clinical significance and frequency of Blastocystis hominis in Turkish patients with hematological malignancy. Acta Medica Okayama 54: 133-136.
  • [17] Stensvold C.R., Christiansen D.B., Olsen K.E.P., Nielsen H.V. 2011. Blastocystis sp. subtype 4 is common in Danish Blastocystis-positive patients presenting with acute diarrhea. American Journal of Tropical Medicine and Hygiene 84: 883-885.
  • [18] Jimenez-Gonzalez D.E., Martinez-Flores W.A., Reyes-Gordillo J., Ramirez-Miranda M.E., Arroyo-Escalante S., Romero-Valdovinos M., Stark D., Souza-Saldivar V., Martinez-Hernandez F., Flisser A. 2012. Blastocystis infection is associated with irritable bowel syndrome in a Mexican patient population. Parasitology Research 110: 1269-1275.
  • [19] Vogelberg C., Stensvold C.R., Monecke S., Ditzen A., Stopsack K., Heinrich-Gräfe U., Pöhlmann C. 2010. Blastocystis sp. subtype 2 detection during recurrence of gastrointestinal and urticarial symptoms. Parasitology International 59: 469-471.
  • [20] Chen T.L., Chan C.C., Chen H.P., Fung C.P., Lin C.P., Chan W.L., Liu C.Y. 2003. Clinical characteristics and endoscoic findings associated with Blastocystis hominis in healthy adults. American Journal of Tropical Medicine and Hygiene 69: 213-216.
  • [21] Rivera W.L., Tan M.A. 2005. Molecular characterization of Blastocystis isolates in the Philippines by riboprinting. Parasitology Research 96: 253-257.
  • [22] Tan M.A.V., Rivera W.L. 2009. Genotypic characterization of Blastocystis isolates in the Philippines. Philippine Journal of Veterinary Medicine 45: 86-94.
  • [23] Banaticla J.E., Rivera W.L. 2011. Detection and subtype identification of Blastocystis isolates from wastewater samples in the Philippines. Journal of Water and Health 9: 128-137.
  • [24] Adao D.E.V., Ducusin R.J.T., Padilla M.A., Rivera W.L. 2016. Molecular characterization of Blastocystis isolates infecting farm animals in Victoria and Pila, Laguna, Philippines. The Philippine Agricultural Scientist (in press).
  • [25] Walsh P.S., Metzger D.A., Higuchi R. 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506-513.
  • [26] Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95-98.
  • [27] Posada D. 2008. jModelTest: phylogenetic model averaging. Molecular Biology and Evolution 25: 1253-1256.
  • [28] Swofford D.L. 2003. PAUP*. Phylogenetic analysis using parsimony (* and other methods). Version 4. Sinauer Associates, Sunderland, Massachusetts.
  • [29] Ronquist F., Huelsenbeck J.P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572-1574.
  • [30] Guindon S., Dufayard J.F., Lefort V., Anisimova M., Hordijk W., Gascuel O. 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology 59: 307-321.
  • [31] Baldo E.T., Belizario V.Y., De Leon W.U., Kong H.-H., Chung D.-I. 2004. Infection status of intestinal parasites in children living in residential institutions in Metro Manila, the Philippines. Korean Journal of Parasitology 42: 67-70.
  • [32] Belleza M.L.B., Cadacio J.L.C., Borja M.P., Solon J.A.A., Padilla M.A., Tongol-Rivera P.N., Rivera W.L. 2015. Epidemiologic study of Blastocystis infection in an urban community in the Philippines. Journal of Environmental and Public Health. doi:10.1155/2015/894297.
  • [33] Stensvold C.R., Suresh G.K., Tan K.S., Thompson R., Traub R.J., Viscogliosi E., Yoshikawa H., Clark C.G. 2007. Terminology for Blastocystis subtypes – a consensus. Trends in Parasitology 23: 93-96.
  • [34] Noël C., Dufernez F., Gerbod D., Edgcomb V.P., Delgado-Viscogliosi P., Ho L.C., Singh M., Wintjens R., Sogin M.L., Capron M. 2005. Molecular phylogenies of Blastocystis isolates from different hosts: implications for genetic diversity, identification of species, and zoonosis. Journal of Clinical Microbiology 43: 348-355.
  • [35] Alfellani M.A., Stensvold C.R., Vidal-Lapiedra A., Onuoha E.S., Fagbenro-Beyioku A.F., Clark C.G. 2013. Variable geographic distribution of Blastocystis subtypes and its potential implications. Acta Tropica 126: 11-18.
  • [36] Forsell J., Granlund M., Stensvold C.R., Clark C.G., Clark G.C., Evengard B. 2012. Subtype analysis of Blastocystis isolates in Swedish patients. European Journal of Clinical Microbiology Infectious Diseases 31: 1689-1696.
  • [37] Li L.H., Zhou X.N., Du Z.W., Wang X.Z., Wang L.B., Jiang J. Y., Yoshikawa H., Steinmann P., Utzinger J., Wu Z., Chen J.X., Chen S.H., Zhang L. 2007. Molecular epidemiology of human Blastocystis in a village in Yunnan province, China. Parasitology International 56: 281-286.
  • [38] Ramirez J.D., Sanchez L.V., Bautista D.C., Corredor A.F., Florez A.C., Stensvold C.R. 2014. Blastocystis subtypes detected in humans and animals from Colombia. Infection, Genetics and Evolution 22: 223-228.
  • [39] Alfellani M.A., Jacob A.S., Perea N.O., Krecek R.C., Taner-Mulla D., Verweij J.J., Levecke B., Tannich E., Clark C.G., Stensvold C.R. 2013. Diversity and distribution of Blastocystis sp. subtypes in non-human primates. Parasitology 140: 966-971.
  • [40] Bart A., Wentink-Bonnema E.M., Gilis H., Verhaar N., Wassenaar C.J., van Vugt M., Goorhuis A., van Gool T. 2013. Diagnosis and subtype analysis of Blastocystis sp. in 442 patients in a hospital setting in the Netherlands. BMC Infectious Diseases 13: 389. doi:10.1186/1471-2334-13-389.
  • [41] El Safadi D., Meloni D., Poirier P., Osman M., Cian A., Gaayeb L., Wawrzyniak I., Delbac F., El Alaoui H., Delhaes L. 2013. Molecular epidemiology of Blastocystis in Lebanon and correlation between subtype 1 and gastrointestinal symptoms. American Journal of Tropical Medicine and Hygiene 88: 1203-1206.
  • [42] Wong K.H., Ng G., Lin R.T., Yoshikawa H., Taylor M.B., Tan K.S. 2008. Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore. Parasitology Research 102: 663-670.
  • [43] Jantermtor S., Pinlaor P., Sawadpanich K., Pinlaor S., Sangka A., Wilailuckana C., Wongsena W., Yoshikawa H. 2013. Subtype identification of Blastocystis spp. isolated from patients in a major hospital in northeastern Thailand. Parasitology Research 112: 1781-1786.
  • [44] Popruk S., Udonsom R., Koompapong K., Mahittikorn A., Kusolsuk T., Ruangsittichai J., Palasuwan A. 2015. Subtype distribution of Blastocystis in Thai-Myanmar Border, Thailand. Korean Journal of Parasitology 53: 13-19.
  • [45] Thathaisong U., Siripattanapipong S., Mungthin M., Pipatsatitpong D., Tan-ariya P., Naaglor T., Leelayoova S. 2013. Identification of Blastocystis subtype 1 variants in the home for girls, Bangkok, Thailand. American Journal of Tropical Medicine and Hygiene 88: 352-358.
  • [46] Lee L.I., Chye T.T., Karmacharya B.M., Govind S.K. 2012. Blastocystis sp.: waterborne zoonotic organism, a possibility? Parasites and Vectors 5: 130. doi: 10.1186/1756-3305-5-130.
  • [47] Abdulsalam A.M., Ithoi I., Al-Mekhlafi H.M., Ahmed A., Surin J., Mak J.W. 2012. Drinking water is a significant predictor of Blastocystis infection among rural Malaysian primary schoolchildren. Parasitology 139: 1014-1020.
  • [48] Leelayoova S., Siripattanapipong S., Thathaisong U., Naaglor T., Taamasri P., Piyaraj P., Mungthin M. 2008. Drinking water: a possible source of Blastocystis spp. subtype 1 infection in schoolchildren of a rural community in central Thailand. American Journal of Tropical Medicine and Hygiene 79: 401-406.
  • [49] Leelayoova S., Rangsin R., Taamasri P., Naaglor T., Thathaisong U., Mungthin M. 2004. Evidence of waterborne transmission of Blastocystis hominis. American Journal of Tropical Medicine and Hygiene 70: 658-662.
  • [50] Ithoi I., Jali A., Mak J., Wan Sulaiman W.Y., Mahmud R. 2011. Occurrence of Blastocystis in water of two rivers from recreational areas in Malaysia. Journal of Parasitology Research. doi:10.1155/2011/123916.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-87e2dc1f-8e2e-4124-bf45-66d1971754e1
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.