Wyniki hodowli tasiemców z rzędu Psudophyllidea in vitro
Treść / Zawartość
Conditions of Psudophyllidea tapeworm cultivation in vitro
Uslovija rosta cestod iz roda Psudophyllidea in vitro
The review covers previous publications of Smyth dealing with cultivation of larval Cestode in vitro. Plecercoides were rearing in axenic conditions in vitro and obtained after 72 hours a mature from of Schistocephalus solidus. The plerocercoids were kept in a tube made of half permeable membrane which was placed in a vessel with fluent medium - horse serum; above vessel was heated to a temperature of 40° C being all the time shaked. The rearing of the coracides, out of Schistocephalus solidus ova obtained in vitro, procecded in water with a temperature or 25° C. The incubation of the coracides took place after 8 days. When the culture was trated with sun rays for several hours the process of the ova operculi opening was accelerated. The best results in cultivating plerocercoids Diphyllobothrium dendriticum in vitro up to a mature from was obtained on a medium containing an extract of duck embryo together with serum, monosacharides and albuminous hydrolysates. The plerocercoids after 6 days of breeding attained the stadium of late gametogenesis and shoved a defective somatic development. Any enrichment of the medium in nutritive substances failed to evoke further progress in the plerocercoids development - viz. formation of vitellous glands. In order to check whether the methods chosen for the development of tapeworms reared in vitro have been correct, cytologie, histological and histochemical tests have been performed. Cultivation in vitro is a contribution to the better understanding of relations and dependency existing between the parasite and its host.
- 1. Bell, E. J., Smyth, J. D.: Cytological and histochemical criteria for evaluating development of Trematodes and Pseudophyllidean Cestodes in vivo and in vitro. - Parasitology, 48, 1, 2, 1958.
- 2. Smyth J. D.: Problems relating to the in vitro cultivation of Pseudophyllidean Cestodes from egg to adult. - Revista Iberica de Parasitologia, Tomo Extraordinario, Marzo, 1958.
- 3. Smyth J. D.: Cultivation and development of larval Cestode Fragments in vitro. - Nature, 181, 1958.
- 4. Smyth J. D.: Maturation of larval Pseudophyllidean Cestodes and strigeid Trematodes under axenic conditions; the significance of nutritional levels in Platyhelminth development. - Annals of the New York Academy of Sciences, 77, 1959.