Evaluation of the genetic stability of plants obtained via somatic embryogenesis in Chrysanthemum x grandiflorum (Ramat./Kitam.)

• Autorzy: Lema-Ruminska J. , Sliwinska E.

Warianty tytułu

PL

Ocena stabilności genetycznej roślin uzyskanych przez embriogenezę somatyczną Chrysanthemum x grandiflorum (Ramat./Kitam.)

• Języki publikacji: EN

Abstrakty

EN

Genetic and phenotypic stability of plants obtained via somatic embryogenesis may be disrupted. The reason can be an indirect regeneration of somatic embryos via callus or a high concentration of growth regulators added at the induction stage of somatic embryos. Somatic embryogenesis of ‘Lady Salmon’ (chimeric) and ‘Lady Vitroflora’ (non-chimeric) cultivars was induced on modified Murashige and Skoog (MS) media, supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (KIN) or 6-benzylaminopurine (BAP). Flow cytometry (FCM) revealed that the plants derived from somatic embryos of both cultivars maintained the ploidy of control plants obtained from the meristem. The colour and pigment content of the inflorescence of plants derived from somatic embryos of ‘Lady Vitroflora’ were similar to the original control plants. However, the ray florets of clones of ‘Lady Salmon’ did not contain carotenoids, characteristic for this cultivar, and consequently produced flowers of different colours. Thus, somatic embryogenesis in chrysanthemums can be applied for separating periclinal chimera components for chimeric cultivars and for receiving an additional source of variation in the breeding of cultivars. In the case of genetically homogeneous cultivars it can be used in production laboratories for cloning plants in vitro via somatic embryogenesis.

PL

Stabilność genetyczna i fenotypowa roślin uzyskanych przez embriogenezę somatyczną może zostać zachwiana. Powodem może być pośrednia regeneracja zarodków somatycznych poprzez kalus lub wysokie stężenie regulatorów wzrostu dodanych na etapie indukcji zarodków somatycznych. Embriogeneza somatyczna chryzantem odmian: ‘Lady Salmon’ (chimera) i ‘Lady Vitroflora’ (niechimera), była indukowana na zmodyfikowanych pożywkach Murashige i Skoog (MS) z dodatkiem 4 mg·dm-3 kwasu 2,4-dwuchlorofenoksyoctowego (2,4-D) i 1 mg·dm-3 kinetyny (KIN) lub 6-benzylaminopuryny (BAP). Cytometria przepływowa (FCM) wykazała, że rośliny pochodzące z zarodków somatycznych obu odmian utrzymują ploidalność roślin kontrolnych uzyskanych z merystemu. Barwa i zawartość barwników w kwiatostanach roślin pochodzących z zarodków somatycznych u ‘Lady Vitroflora’ były podobne do roślin kontrolnych. Jednak kwiaty języczkowate klonów ‘Lady Salmon’ nie zawierały karotenoidów, charakterystycznych dla tej odmiany, a w konsekwencji tworzyły kwiaty w różnych barwach. Tak więc embriogeneza somatyczna u chryzantem może być stosowana do oddzielania składników chimery peryklinalnej u odmian będących chimerami i otrzymania dodatkowego źródła zmienności w hodowli odmian. Natomiast u odmian jednorodnych genetycznie może być stosowana w laboratoriach produkcyjnych do klonowania roślin w warunkach in vitro poprzez embriogenezę somatyczną.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2015
• Tom: 14
• Numer: 3
• Opis fizyczny: p.131-139,fig.,ref.

Twórcy

autor

Lema-Ruminska J.

• Laboratory of Biotechnology, Department of Ornamental Plants and Vegetable Crops, UTP University of Technology and Life Sciences, Bernardynska 6, 85-029 Bydgoszcz, Poland

autor

Sliwinska E.

• UTP University of Technology and Life Sciences, Bernardynska 6, 85-029 Bydgoszcz, Poland

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