Relationship between activity of matrix mettaloproteinasa -2 and -9 (MMP-2, MMP-9) and ischemia induced neurogenesis in adult gerbil hipoccampus

• Autorzy: Wojcik L. , Zalewska T.
• Języki publikacji: EN

Abstrakty

EN

In physiological conditions proteolytic remodeling of extracellular matrix (ECM) participates in the stem cells development. Signal derived from ECM may activate specifi c intracellular signaling pathways which involve focal adhesion kinase (FAK), a key component responsible for the fl ow of information to the cell. In our study we checked if there is a spatial and temporal relationship between activity of two matrix metalloproteinases (MMP-2 and MMP-9) and neural stem-cell development in gerbil hippocampus 1, 2 and 4 weeks after short-term global ischemia. Furthermore we also investigate the activation of FAK. Our results show that after the ischemia there is a signifi cant increase in the number of proliferating cells in DG which correlates with the activation of MMPs. Whereas in the damaged CA1 pyramidal layer MMP-2 and -9 activity decreases as a result of delayed death of neurons. In the same region we have also noticed a few immature neurons (NF-200 positive) 2 and 4 weeks after reperfusion, however they did not attain maturity. On the other hand we have noticed higher MMPs activity in the astrocytes surrounding the CA1 region. The diverse localization of MMP-2 and -9 activity in both cell types may be due to their different functions – fi rstly with the proliferation of newborn cells and secondly with the creation of the environment while the formation of post injury glial scar. Supported by MSRHE grants: 1266/P01/2006/31

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.310

Twórcy

autor

Wojcik L.

• NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland

autor

Zalewska T.

• NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland