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2015 | 18 | 4 |
Tytuł artykułu

Selection and electrophoretic characterization of Salmonella enterica subsp. enterica biocide variants resistant to antibiotics

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The proposed research outlines a serious common concern of Salmonella resistance to antimicrobials following prolonged exposure to the disinfectants (biocides). These phenotypes of bacteria could potentially result in hard to treat infections. Typical for avian sources, biocide sensitive S. enterica subsp. enterica serovars: Typhimurium, Enteritidis, Virchow and Zanzibar and their isogenic biocide-tolerant variants were studied in order to investigate bacteriostatic effect of two commercially available biocide formulations: potassium peroxymonosulfate (P) and dodecylamine based structure (triamine, D). We found that cultivating of the bacteria in the medium supplemented with a blend containing P did not influence their antibiotic susceptibility pattern. In contrast, tolerance of bacteria to D compound resulted in resistance to co-trimoxazole, cefotaxime and ciprofloxacin of which two cefotaxime and ciprofloxacin are used commonly for the treatment of invasive Salmonella infections in humans. The dependency between OMP patterns and the level of Salmonella survival in media containing the biocides was observed merely in serovar Typhimurium. In conclusion, these results suggest that Salmonella strains challenged by prolonged treatment with the disinfectants become resistant to antibiotics, however it depends on Salmonella serovar and the chemical used. This paper also highlights the loop-mediated isothermal amplification (LAMP) as a technique that offers great benefits to microbiological detecting of Salmonella species in the samples.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
18
Numer
4
Opis fizyczny
p.725-732,fig.,ref.
Twórcy
  • Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland
autor
  • Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland
autor
  • Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland
autor
  • Department of Genetics and Cell Physiology, Institute of Experimental Biology, University of Wroclaw, Kanonia 6/8, 50-328 Wroclaw, Poland
autor
  • Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland
autor
  • Department of Biotechnology and Microbiology, Faculty of Food Science and Nutrition, University of Life Sciences, Wojska Polskiego 48, 60-627 Poznan, Poland
autor
  • Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland
  • Department of Pathogens’ Biology and Immunology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland
  • Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland
Bibliografia
  • Afema JA, Mather AE, Sischo WM (2014) Antimicrobial resistance profiles and diversity in Salmonella from humans and cattle, 2004-2011. Zoonoses Public Health.
  • Angulo FJ, Baker NL, Olsen SJ, Anderson A, Barrett TJ (2004) Antimicrobial use in agriculture: controlling the transfer of antimicrobial resistance to humans. Sem Pediatr Infect Dis 15: 78-85.
  • Chapman JS (2003) Biocide resistance mechanisms. Int Biodeter Biodegr 51: 133-138.
  • Condell O, Iversen C, Cooney S, Power KA, Walsh C, Burgess C, Fanning S (2012) Efficacy of biocides used in the modern food industry to control Salmonella enterica, and links between biocide tolerance and resistance to clinically relevant antimicrobial compounds. Appl Environ Microbiol 78: 3087-3097.
  • European Committee on Antimicrobial Susceptibility Testing (2015) Breakpoint tables for interpretation of MICs and zone diameters Version 5.0, valid from 2015-01-01, 1-78.
  • Eurosurveillance editorial team (2013) ECDC the annual epidemiological report 2012. Eurosurveill 18: 20418.
  • Futoma-Kołoch B, Ksiażczyk M, Bugla-Płoskońska G (2013) The risk of Salmonella resistance following exposure to common disinfectants: an emerging problem. Biology International 53, 54-66.
  • Futoma-Kołoch B, Migdał I, Burzyński A, Jankowska M, Woźniakowski G (2014) LAMP – szybka i specyficzna detekcja patogenów. Laboratorium – Przegląd Ogólnopolski 5-6, 32-37.
  • Giraud E, Baucheron S, Cloeckaert A (2006) Resistance to fluoroquinolones in Salmonella: emerging mechanisms and resistance prevention strategies. Microbes Infect 8: 1937-1944.
  • Karatzas KA, Webber MA, Jorgensen F, Woodward MJ, Piddock LJ, Humphrey TJ (2007) Prolonged treatment of Salmonella enterica serovar Typhimurium with commercial disinfectants selects for multiple antibiotic resistance, increased efflux and reduced invasiveness. J Antimicrob Chemother 60: 947-955.
  • Karatzas KA, Randall LP, Webber M, Piddock LJ, Humphrey TJ, Woodward MJ, Coldham NG (2008) Phenotypic and proteomic characterization of multiply antibiotic-resistant variants of Salmonella enterica serovar Typhimurium selected following exposure to disinfectants. Appl Environ Microbiol 74: 1508-1516.
  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
  • Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28: E63.
  • Olliver A, Vallé M, Chaslus-Dancla E, Cloeckaert A (2005) Overexpression of the multidrug efflux operon acrEF by insertional activation with IS1 or IS10 elements in Salmonella enterica serovar typhimurium DT204 acrB mutants selected with fluoroquinolones. Antimicrob Agents Chemother 49: 289-301.
  • Ricci V, Tzakas P, Buckley A, Piddock LJ (2006) Ciprofloxacin-resistant Salmonella enterica serovar Typhimurium strains are difficult to select in the absence of AcrB and TolC. Antimicrob Agents Chemother 50: 38-42.
  • Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150: 76-85.
  • Su LH, Chiu CH, Chu C, Ou JT (2004) Antimicrobial resistance in nontyphoid Salmonella serotypes: a global challenge. Clin Infect Dis 39: 546-551.
  • Whitehead RN, Overton TW, Kemp CL, Webber MA (2011) Exposure of Salmonella enterica Serovar Typhimurium to high level biocide challenge can select multidrug resistant mutants in a single step. PLoS One 6: e22833.
Typ dokumentu
Bibliografia
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Identyfikator YADDA
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