Selection and electrophoretic characterization of Salmonella enterica subsp. enterica biocide variants resistant to antibiotics

• Autorzy: Futoma-Koloch B. , Ksiazczyk M. , Korzekwa K. , Migdal I. , Pawlak A. , Jankowska M. , Kedziora A. , Dorotkiewicz-Jach A. , Bugla-Ploskonska C.
• Języki publikacji: EN

Abstrakty

EN

The proposed research outlines a serious common concern of Salmonella resistance to antimicrobials following prolonged exposure to the disinfectants (biocides). These phenotypes of bacteria could potentially result in hard to treat infections. Typical for avian sources, biocide sensitive S. enterica subsp. enterica serovars: Typhimurium, Enteritidis, Virchow and Zanzibar and their isogenic biocide-tolerant variants were studied in order to investigate bacteriostatic effect of two commercially available biocide formulations: potassium peroxymonosulfate (P) and dodecylamine based structure (triamine, D). We found that cultivating of the bacteria in the medium supplemented with a blend containing P did not influence their antibiotic susceptibility pattern. In contrast, tolerance of bacteria to D compound resulted in resistance to co-trimoxazole, cefotaxime and ciprofloxacin of which two cefotaxime and ciprofloxacin are used commonly for the treatment of invasive Salmonella infections in humans. The dependency between OMP patterns and the level of Salmonella survival in media containing the biocides was observed merely in serovar Typhimurium. In conclusion, these results suggest that Salmonella strains challenged by prolonged treatment with the disinfectants become resistant to antibiotics, however it depends on Salmonella serovar and the chemical used. This paper also highlights the loop-mediated isothermal amplification (LAMP) as a technique that offers great benefits to microbiological detecting of Salmonella species in the samples.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2015
• Tom: 18
• Numer: 4
• Opis fizyczny: p.725-732,fig.,ref.

Twórcy

autor

Futoma-Koloch B.

• Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland

autor

Ksiazczyk M.

• Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland

autor

Korzekwa K.

• Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland

autor

Migdal I.

• Department of Genetics and Cell Physiology, Institute of Experimental Biology, University of Wroclaw, Kanonia 6/8, 50-328 Wroclaw, Poland

autor

Pawlak A.

• Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland

autor

Jankowska M.

• Department of Biotechnology and Microbiology, Faculty of Food Science and Nutrition, University of Life Sciences, Wojska Polskiego 48, 60-627 Poznan, Poland

autor

Kedziora A.

• Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland

autor

Dorotkiewicz-Jach A.

• Department of Pathogens’ Biology and Immunology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland

autor

Bugla-Ploskonska C.

• Department of Microbiology, Institute of Genetics and Microbiology, University of Wroclaw, Przybyszewskiego 63-77, 51-148 Wroclaw, Poland

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Identyfikatory

DOI

10.1515/pjvs-2015-0094