Effect of medium type and plant growth regulators on the in vitro shoot proliferation of Cotinus coggygria Scop. 'Royal Purple'

• Autorzy: Jaygrad E. , Ilczuk A. , Mikos M. , Jagiello-Kubiec K.

Warianty tytułu

PL

Wpływ zestawu soli mineralnych, pH pożywki i regulatorów wzrostu na regenerację pędów Cotinus coggygria Scop. 'Royal Purple' in vitro

• Języki publikacji: EN

Abstrakty

EN

The smoke bush (Cotinus coggygria Scop.) is a popular and highly demanded ornamental shrub whose propagation can be problematic. The aim of the study was to test the effect of the mineral salt composition in the medium, concentration of plant growth regulators and the medium pH on the in vitro multiplication and shoot growth of C. coggygria Scop. ‘Royal Purple’. Shoot tips were cultured on the following media: Anderson (AN), Murashige and Skoog (MS) (full- , half- and quarter strength), Nitsch and Nitsch (NN), Quoirin and Lepoivre (QL) or Lloyd and McCown (WPM). Media were supplemented with 6-benzyladenine (BA) in combinations with 1-naphthaleneacetic acid (NAA). The medium type and a concentration of the cytokinin significantly affected the regeneration rate of explants, the number, length and weight of axillary shoots as well as callus growth. The MS medium containing full strength of mineral salts with pH 5.8 was the best. High shoot proliferation (100%) and the highest number of shoots per explant (4.6) were obtained due to the use of 1.0 mg·dm-3 BA in combination with 0.1 mg·dm-3 NAA .

PL

Perukowiec podolski (Cotinus coggygria Scop.) jest popularnym i poszukiwanym na rynku krzewem ozdobnym, którego rozmnażanie może sprawiać problemy. Celem badań było określenie wpływu zestawu soli mineralnych, stężenia regulatorów wzrostu oraz pH pożywki na regenerację i wzrost in vitro perukowca podolskiego C. coggygria Scop. ‘Royal Purple’. Wierzchołkowe fragmenty pędów wykładano na pożywki wg Andersona (AN), Murashige i Skoog (MS) (pełny zestaw, ½ lub ¼), Nitsch i Nitsch (NN), Quorin i Lepoivre (QL) oraz Lloyd i McCown (WPM). Pożywki wzbogacano w 6-benzyloadeninę (BA) w różnych stężeniach w kombinacji z kwasem naftylo-1- octowym (NAA). Wykazano istotny wpływ rodzaju pożywki i stężenia zastosowanej cytokininy na liczbę, długość i świeżą masę zregenerowanych pędów i wzrost kalusa. Najlepsze efekty uzyskano na pożywce MS o pełnym składzie makroskładników i pH 5,8. W obecności 1,0 mg·dm-3 BA i 0,1 mg·dm-3 NAA eksplantaty wierzchołkowe regenerowały w 100% i odznaczały się najwyższym współczynnikiem rozmnażania (4,6).

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2012
• Tom: 11
• Numer: 5
• Opis fizyczny: p.143-151,fig.,ref.

Twórcy

autor

Jaygrad E.

• Department of Ornamental Plants, Warsaw University of Life Sciences - SGGW, ul. Nowoursynowska 166, 02-787 Warsaw, Poland

autor

Ilczuk A.

• Warsaw University of Life Sciences - SGGW, Warsaw, Poland

autor

Mikos M.

• Warsaw University of Life Sciences - SGGW, Warsaw, Poland

autor

Jagiello-Kubiec K.

• Warsaw University of Life Sciences - SGGW, Warsaw, Poland

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