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2012 | 30 | 2 |
Tytuł artykułu

Adptation of primer extension method for monitoring of scrapie-associated genotypes in sheep PRNP gene

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The paper describes adaptation of primer extension method for genotyping scrapie-associated codons 136, 141 and 156 in sheep PRNP gene. As the method failed to genotype the polymorphism in codon 171 in some samples, this polymorphism was excluded from the assay. Such genotyping problems were not observed in earlier studies describing the method and cannot be easily explained. However, full accordance of genotypes in codons 136, 141 and 156 with sequencing results was obtained and thus the method can be recommended for routine application. Simultaneously, we strongly recommend to pay special attention when using the minisequencing method for genotyping codon 171 in sheep PRNP.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
30
Numer
2
Opis fizyczny
p.151-158,fig.,ref.
Twórcy
autor
  • Department of Animal Cytogenetics and Molecular Genetics of the National Research Institute for Animal Production, Krakowska 1, 32-083 Balice, Cracow, Poland
autor
autor
Bibliografia
  • BOSSERS A., VRIES R., SMITS M., 2000 – Susceptibility of Sheep for Scrapie as Assessed by In Vitro Conversion of Nine Naturally Occurring Variants of PrP. Journal of Virology 74, 1407-1414.
  • DEARMOND S. J., PRUSINER S. B., 1995 – Etiology and Pathogenesis of Prion Diseases. American Journal of Pathology 146, 785-811.
  • FEDIAEVSKY A., TONGUE S., NÖREMARK M., CALAVAS C., RU G., HOPP P., 2008 – A descriptive study of the prevalence of atypical and classical scrapie in sheep in 20 European countries.BMC Veterinary Research 4, 19.
  • GARCIA-CRESPO D., OPORTO B., GOMEZ N., NAGORE D., BENEDICTO L., JUSTE R.A.,HURTADO A., 2004 – PrP polymorphisms in Basque sheep breeds determined by PCR-restriction fragment length polymorphism and real-time PCR. Veterinary Record 154, 717-722.
  • GOLDMANN W., HOUSTON F., STEWART P., PERUCCHINI M., FOSTER J., HUNTER N., 2006 – Ovine prion protein variant A136 R154 L168 Q171 increases resistance to experimental challenge with bovine spongiform encephalopathy agent. Journal of General Virology 87, 3741-3745.
  • GROSCHUP M., HARMEYER S., PFAFF E., 1997 – Antigenic features of prion proteins of sheep and other mammalian species. Journal of Immunological Methods 207, 89-101.
  • HORIUCHI M., NEMOTO T., ISHIGURO N., FURUOKA H., MOHRI S., SHINAGAWA M.,2002 – Biological and Biochemical Characterization of Sheep Scrapie in Japan. Journal of Clinical Microbiology 40, 3421-3426.
  • LAU A., YAM A. Y., MICHELITSCH M., WANG X., GAO C., GOODSON R. J., SHIMIZU R., TIMOTEO G., HALL J., MEDINA-SELBY A., COIT D., MCCOIN C., PHELPS B., WU P., HU C., CHIEN D., PERETZ D., 2007 – Characterization of prion protein (PrP)-derived peptides that discriminate full-length PrPSc from PrPC. Proceedings of the National Academy of Sciences of the USA 104, 11551-11556.
  • PIESTRZYŃSKA-KAJTOCH A., OCZKOWICZ M., NATONEK-WIŚNIEWSKA M.,PIÓRKOWSKA K., KAWECKA M., KOZUBSKA-SOBOCIŃSKA A., KNAPIK J., KRUPIŃSKI J., REJDUCH B., 2009 – International Scientific Conference: Transmissible Spongiform Encephalopathies in Farm Animals – Diagnosis and Prevention. Balice, Poland, 28-29 September,Book of Abstracts p. 57.
  • POLAK M.P, LARSKA M., LANGEVELD J.P., BUSCHMANN A., GROSCHUP M.H.,ZMUDZINSKI J.F., 2010 – Diagnosis of the first cases of scrapie in Poland. Veterinary Journal 186, 47-52.
  • TONGUE S.C., WILESMITH J.W., COOK C.J., 2004 – Frequencies of prion protein (PrP) genotypem and distribution of ages in 15 scrapie-affected flocks in Great Britain. Veterinary Record 154, 9-16.Erratum in: Vet. Rec. 154, 116.
  • MOUM T., OLSAKER I., HOPP P., MOLDAL T., VALHEIM M., MOUM T., BENESTAD S.L.,2005 – Polymorphisms at codons 141 and 154 in the ovine prion protein gene are associated with scrapie Nor98 cases. Journal of General Virology 86, 231-235.
  • WIERZBICKA A., DEPTUŁA W., 2008 – Rola układu odpornościowego w patogenezie chorób prionowych. (The role of the immune system in the pathogenesis of prion diseases). In Polish with Emglish summary. Postępy Higieny i Medycyny Doświadczalnej 62, 166-173.
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PL
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.agro-862e466e-1175-4a1a-8c9a-4f0781907433
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