Metodyka eksperymentalnego zarażania kleszczy toksoplazmozą

• Autorzy: Szymanski S.

Warianty tytułu

EN

Methods of experimental infection of ticks with toxoplasma

RU

Metodika ehksperimental'nogo zarazhenija kleshhejj toksoplazmozom

• Języki publikacji: PL

Abstrakty

EN

In experimental investigations on elucidation of the role of ectoparasites in transmitting agents of various infectious and invastive diseases, the method of infecting the vectors is of great importance. In such experiments the laboratorial animals (donators) are in most cases the source of infection for parasitic arthropods. Such a method, however, is very expensive and, for various reasons gives not always positive results. First of all, in relation to some diseases (e.g. toxoplasmosis) the feeding on donators gives no full guarantee for the infection of arthropods. In this connection a number of methods of artificial infection of insects and ticks withs various pathogenic microorganisms was introduced to the laboratory practice. Among others the following methods belong to them: the feeding of arthropods on chick embryos and the capillary method. One decided to use both these methods for infecting ticks with toxoplasma. Using the first method a hole was made in the shell (over the air sac) of 11-12 days old chick embryos infected 3-4 days before witr toxoplasma, and ticks were placed on the chorioallantoic membrane where they saturated themsleves with embryos' blood. In this way the following tick species were infected: Argas persicus, Ornithodoros lahorensis, O. papillipes and O. coniceps. The second method was based on compulsory feeding of ticks. These latter were fastened to strips of plastic clay placed on slides, and glass capillars filled with concentrated (through centrifugation) toxoplasma exudate, were put on their proboscis. By means of this method the following ticks were infected: Hyalomma asiaticum, Dermacentor marginatus and D. pictus. Despite some imperfection both methods gave very good results: by means of them a high degree of infection of the ticks was aitained.

• Wydawca: -
• Czasopismo: Wiadomości Parazytologiczne
• Rocznik: 1960
• Tom: 06
• Numer: 2-3
• Opis fizyczny: s.147-156,tab.,fot.,bibliogr.

Twórcy

autor

Szymanski S.

• Zakład Parazytologii Lekarskiej, Państwowy Zakład Higieny, Warszawa

Bibliografia

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