Oil was extracted from the dehulled seeds of Lagneraria siceraria (bottle gourd) and analysed for physico-chemical properties, as well a fatty acid composition. Standard procedures were employed in all analysis. The seed oil was liquid at room temperature with percentage yield (23.65%). The oil was characterized in terms of specific gravity (0.918 g/cm3), refractive index (1.34), viscosity (26.46 X 103 poise), melting point (11-14.5 °C), moisture content (0.18%), saponification value (203.36 mg KOH/g), unsaponifiable matter (7.13%), iodine value (46.1 g/100g), peroxide value (7.5 meq/kg), free fatty acid value (18.42%), acid value (60.02 mg KOH/g) and ester value (143.34 mg KOH/g). It was also classified as non- drying (iodine value ˂115 g/100 g). The peroxide value indicates that the oil is less prone to rancidity with iodine value less than 30meq/kg. The high saponification value qualifies it for use in the manufacture of soaps and shampoos. Four classes of fatty acid were identified in the oil: palmitic acid (C16:1) (13.5 ± 0.21), stearic acid (C18:1) (6.5 ± 0.96), oleic acid (C18:1) (11.6 ± 0.62) and linoleic acid (C18:2) (68.4 ± 0.13). Linoleic acid was the most abundant fatty acid in the oil. The fatty acid content of the oil reveals that L. Siceraria seed oil could be a rich source of oil for domestic and industrial purposes if exploited.