In vitro propagation and biosynthesis of steroidal sapogenins from various morphogenetic stages of Moringa oleifera Lam., and their antioxidant potential

• Autorzy: Mathur M. , Yadav S. , Katariya P.K. , Kamal R.
• Języki publikacji: EN

Abstrakty

EN

Moringa oleifera is a highly valued medicinal plant. The present research reports callus cultures of M. oleifera Lam., established from seeds and nodal segments on Murashige and Skoog’s (MS) medium using different concentrations and combinations of auxins and cytokinins. Best induction of callus was observed at BAP:IBA (3 mg l⁻¹ each). Shooting and rooting from callus in terms of morphogenesis were observed in MS media supplemented with BAP:KN (2:0.2 mg l⁻¹) and IBA:NAA (3:0.5 mg l⁻¹), respectively. Multiple shooting was observed at treatment dose of BAP:NAA:IAA (1:1:0.2 mg l⁻¹). Regenerated shoots were rooted and mature plants were established, acclimatized, and thrived in greenhouse conditions. Over 95 % of plantlets survived after transplanting plantlets into trays with a mixture of sand and perlite (2:1) for 20 days. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of bioactive constituents of this medicinal plant. Further qualitative and quantitative production of steroidal sapogenins (diosgenin and tigogenin) from various morphogenetic stages was studied using TLC, PTLC, IR spectra, HPLC and GC–MS analysis. Steroidal sapogenins were maximum in the callus associated with rooting. Various stages were further analyzed for their antioxidant potential.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 07
• Opis fizyczny: p.1749-1762,fig.,ref.

Twórcy

autor

Mathur M.

• Laboratory of Medicinal Plant Biotechnology, Department of Botany, University of Rajasthan, 302004 Jaipur, India

autor

Yadav S.

• Laboratory of Medicinal Plant Biotechnology, Department of Botany, University of Rajasthan, 302004 Jaipur, India

autor

Katariya P.K.

• Laboratory of Medicinal Plant Biotechnology, Department of Botany, University of Rajasthan, 302004 Jaipur, India

autor

Kamal R.

• Laboratory of Medicinal Plant Biotechnology, Department of Botany, University of Rajasthan, 302004 Jaipur, India

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Identyfikatory

DOI

10.1007/s11738-014-1549-1