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2014 | 36 | 07 |

Tytuł artykułu

In vitro propagation and biosynthesis of steroidal sapogenins from various morphogenetic stages of Moringa oleifera Lam., and their antioxidant potential

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Moringa oleifera is a highly valued medicinal plant. The present research reports callus cultures of M. oleifera Lam., established from seeds and nodal segments on Murashige and Skoog’s (MS) medium using different concentrations and combinations of auxins and cytokinins. Best induction of callus was observed at BAP:IBA (3 mg l⁻¹ each). Shooting and rooting from callus in terms of morphogenesis were observed in MS media supplemented with BAP:KN (2:0.2 mg l⁻¹) and IBA:NAA (3:0.5 mg l⁻¹), respectively. Multiple shooting was observed at treatment dose of BAP:NAA:IAA (1:1:0.2 mg l⁻¹). Regenerated shoots were rooted and mature plants were established, acclimatized, and thrived in greenhouse conditions. Over 95 % of plantlets survived after transplanting plantlets into trays with a mixture of sand and perlite (2:1) for 20 days. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of bioactive constituents of this medicinal plant. Further qualitative and quantitative production of steroidal sapogenins (diosgenin and tigogenin) from various morphogenetic stages was studied using TLC, PTLC, IR spectra, HPLC and GC–MS analysis. Steroidal sapogenins were maximum in the callus associated with rooting. Various stages were further analyzed for their antioxidant potential.

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  • Laboratory of Medicinal Plant Biotechnology, Department of Botany, University of Rajasthan, 302004 Jaipur, India
  • Laboratory of Medicinal Plant Biotechnology, Department of Botany, University of Rajasthan, 302004 Jaipur, India
  • Laboratory of Medicinal Plant Biotechnology, Department of Botany, University of Rajasthan, 302004 Jaipur, India
  • Laboratory of Medicinal Plant Biotechnology, Department of Botany, University of Rajasthan, 302004 Jaipur, India


  • Agarwal B, Mehta A (2009) Antiasthmatic activity of Moringa oleifera: a clinical study. Indian J Pharmacol 40(1):28–31
  • Akbas F, Işikalan C, Namli S (2008) Callus induction and plant regeneration from different explants of Actinidia deliciosa. Appl Biochem Biotechnol 158:470–475
  • Ali A, Naz S, Iqbal J (2007) Effect of different explants and media compositions for efficient somatic embryogenesis in sugarcane (Saccharum officinarum). Pak J Bot 39:1961–1977
  • Badmis S, Gupta MK, Suresh B (2003) Antioxidant activity of the ethanolic extract of Striga orobanchiodes. J Ethanopharmacol 85:227–230
  • Barz W, Reinhard E, Zenk MH (1977) Plant tissue culture and its biotechnological application. Springer, Berlin
  • Benjamin S, Yona T, Victor G, Tanya G, Yiftach V (2009) Vegetative micro-cloning to sustain biodiversity of threatened species. In Vitro Cell Develop Biol Plant 45:65–71
  • Brand-Williams W, Cuvellier ME, Berset C (1995) Use of free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28:25–30
  • Cook NC, Samman S (1996) Flavonoids-chemistry, metabolism, cardioprotective effects and dietary sources. Nutr Biochem 7:66–76
  • Coppen JJW (1979) Steroids from plants to pills—the changing picture. Trop Sci 21:125–141
  • Endress R (1994) Plant cell biotechnology. Springer, Germany
  • Fakurazi S, Nanthini U, Hairuszah I (2008) Hepatoprotective and antioxidant action of Moringa oleifera Lam. against acetaminophen induced hepatotoxicity in rats. Int J Pharmocol 4(4):270–275
  • Finkel T, Holbrook NJ (2000) Oxidants, oxidative stress and the biology of ageing. Nature 408:239–247
  • Grossmann K (1988) Plant cell suspensions for screening and studying the mode of action of plant growth retardants. Adv Cell Cult 6:89–136
  • Guevara AP, Vargas C, Sakurai H, Fujiwara Y, Hashimoto K, Maoka T, Kozuka M, Ito Y, Tokuda Y, Nishino H (1999) Antitumour promoter from Moringa oleifera Lam. Mutat Res 440:181–188
  • Hall JB, Walker DH (1991) Balanites aegyptiaca Del.—a monograph. School of Agricultural and Forest Science, University of Wales, Banger
  • Handley LW, Chambliss OL (1979) In vitro propagation of Cucumis sativus L. Hortic Sci 14:22–23
  • Hartwell JL (1967–1971) Plant used against cancer: a survey. Lloydia, pp 30–34
  • Heble MR, Narayanaswami S, Chadha MS (1968) Diosgenin and β-sitosterol: isolation from Solanum xanthocarpum tissue cultures. Science 161:38, 46, 1145
  • Heftman E (1967) Biochemistry of steroidal saponins and glycoalkaloids. Lloydia 30:209–230
  • Heftman E (1974) Recent progress in the biochemistry of plant steroids other than sterols (saponins, glycoalkaloids, pregnane derivatives, cardiac glycosides and sex hormones). Lipids 9:629–639
  • Hussey G (1975) Propagation of Hyacinths by tissue culture. Scientia Horti 3:21–28
  • Ibrahim RK, Edgar H (1976) Phenolic synthesis in Perilla cell suspension cultures. Phytochemistry 15:129–131
  • Islam S, Jahan MAA, Khatun R (2005) In vitro regeneration and multiplication of year round fruit bearing Moringa oleifera Lam. J Biol Sci 5:145–148
  • Jabeen R, Shahid M, Jamil A, Ashraf M (2008) Microscopic evaluation of the antimicrobial activity of seed extracts of Moringa Oleifera. Pak J Bot 40:1349–1358
  • Kadkade PG, Rolz C, Dwyer JD (1976) Steroidal sapogenins of the tubers of Dioscoreaceae: a chemotaxonomic study. Lloydia 39:416–419
  • Kantharajah AS, Dodd W (1991) A rapid clonal propagation of Moringa oleifera Lam., using tissue culture. South Indian Hortic 39:224–228
  • Karadi RV, Palkar MB, Gaviraj EN, Gadge NB, Mannur VS, Alagawadi KR (2008) Antiurolithiatic property of Moringa oleifera Root Bark. Pharma Biol 46:861–865
  • Kaul B, Staba EJ (1968) Dioscorea tissue cultures. I. Biosynthesis and isolation of diosgenin from Dioscorea deltoidea callus and suspension cells. Lloydia 31:171–179
  • Kevers C, Franck T, Strasser RJ, Dommes J, Gaspar T (2004) Hyperhydricity of micro propagated shoots: a typically stress induced change of physiological state. Plant Cell Tissue Organ Cult 77:181–191
  • Khan MS, Usman M, Lilla MI (2006) Facile plant regeneration from tomato leaves induced with spectinomycin. Pak J Bot 38:947–952
  • Khanna P, Jain SC (1973) Diosgenin, gitogenin and tigogenin from Trigonella foenum-graecum L. tissue cultures. Lloydia 36:96–98
  • Kim YJ, Hasegawa PM, Bressan RA (1981) In vitro propagation of Hyacinth. Hortic Sci 16:645–647
  • King P (1976) Utilization of 2, 4-D by steady state cell cultures of Acer pseudoplatones. J Exp Biol 27:1053–1072
  • Liu MJ, Wang Z, Ju Y, Wong RN, Wu QY (2005) Diosgenin induces cell cycle arrest and apoptosis in human leukemia K562 cells with the disruption of Ca²⁺ homeostasis. Cancer Chemother Pharmacol 55:79–90
  • Makris DP, Kefalas P (2001) Effect of principal polyphenolic components in relation to antioxidant characteristics of aged red wines. J Agric Food Chem 49:5736–5742
  • Marfori EC (2010) Clonal micropropagation of Moringa oleifera. Philipp Agric Sci 93:4
  • Mercier H, Kerbauy GB (1992) In vitro multiplication of Vriesea fosteriana. Plant Cell Tissue Organ Cult 30:247–249
  • Mercier H, Kerbauy GB (1993) Micropropagation of Dyckia macedoi—an endangered endemic Brazilian bromeliad. Bot Gard Micropropag News 1:70–72
  • Murashige T (1974) Plant propagation through tissue cultures. Ann Rev Plant Physiol 25:135–166
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497
  • Nieves MC, Aspuria ET (2011) Callus induction in cotyledons of Moringa oleifera Lam. Philipp Agric Sci 94:239–247
  • Offord CA, Campbell LC (1992) Micropropagation of Telopea speciosissima R. Br (Proteaceae). 2: Rhizogenesis and acclimatization to ex vitro conditions. Plant Cell Tissue Organ Cult 29:223–230
  • Oleszek WA (2002) Chromatographic determination of plant saponins. J Chromatogr 967:147–162
  • Parejo I, Viladomat F, Bastida J, Rosas-Romero A, Flerlage N, Burillo J, Codina C (2002) Comparison between the radical scavenging activities and antioxidant activity of six distilled and nondistilled mediterranean herbs and aromatic plants. J Agric Food Chem 50:6882–6890
  • Petiard V, Courtois D (1983) Recent advances in research for novel alkaloids in Apocynaceae tissue cultures. Physiol Veg 21:217–227
  • Reinert J, Bajaj YPS (eds) (1977) Plant cell, tissue and organ culture. Springer, Berlin
  • Roman ID, Thewles A, Coleman R (1995) Fractionation of livers following diosgenin treatment to elevate biliary cholesterol. Biochem Biophys Acta 1255:77–81
  • Safer AM, al-Nughamish AJ (1999) Hepatotoxicity induced by the anti-oxidant food additive, butylated hydroxytoluene (BHT), in rats: an electron microscopical study. Histol Histopathol 14:391–406
  • Saini RK, Shetty NP, Giridhar P, Ravishankar GA (2012) Rapid in vitro regeneration method for Moringa oleifera and performance evaluation of field grown nutritionally enriched tissue cultured plants. 3 Biotech 2:187–192
  • Sanchez GL, Madina JC, Soto RR (1972) Spectrophotometric determination of diosgenin in Dioscorea composite following TLC. Analyst 97:973
  • Schripsema J, Meijer AH, Van IF, Ten HJG, Verpoorte R (1990) Dissimilation cures as a simple method for the characterization of growth of plant cell suspension cultures. Plant Cell Tissue Organ Cult 22:55–64
  • Sohai O, Shuler I (1984) Environmental parameters influencing phenolic production by batch culture of Nicotiana tobacum. Biotechnol Bioeng 26:11–120
  • Staba EJ (1980) Secondary metabolism and biotransformation in plant tissue. Plant tissue culture as a source of biochemical’s. CRC Press, Florida, pp 59–67
  • Stephenson KK, Fahey JW (2004) Development of tissue culture methods for the rescue and propagation of endangered Moringa spp. Germplasm. Econ Bot 58:116–124
  • Sugano N, Iwata R, Nischi A (1975) Formation of phenolic acids in carrot cell suspensions. Phytochemistry 14:1205–1207
  • Sulaiman MR, Zakaria ZA, Bujarimin AS, Somchit MN, Israf DA, Moin S (2008) Evaluation of Moringa oleifera aqueous extract for antinociceptive and anti-inflammatory activities in animal models. Pharma Biol 46(12):838–845
  • Thorpe TA, Patel KR (1984) Clonal propagation: adventitious buds. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants. Academic Press, New York, pp 49–60
  • Tomita Y, Uomori A, Minato H (1970) Steroidal sapogenins and sterols in tissue cultures of Dioscorea tokoro. Phytochemistry 9:111
  • Torrey JG (1966) Morphogenesis in relation to chromosomal constitution in long-term plant tissue cultures. Plant Physiol 20:265
  • Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39:44–84
  • Verma SC, Banerji R, Mishra G, Nigam SK (1976) Nutritional value in Moringa. Curr Sci 45:769–770
  • Wall ME (1960) Steroidal sapogenins and derived steroid hormones. Anne Perfumes Aromat 75:63–73
  • Wang W, Zhao X, Zhuang G, Wang S, Chen F (2008) Simple hormonal regulation of somatic embryogenesis and/or shoot organogenesis in caryopsis cultures of Pogonatherum paniceum (Poaceae). Plant Cell Tissue Organ Cult 95:57–67
  • Wiermann R (1981) Secondary plant products and cell and tissue differentiation. The Biochemistry of Plants, vol 7. Academic Press Inc., San Diego, pp 86–116
  • Woodward AW, Bartel B (2005) Auxin: regulation, action, and interaction. Ann Bot (Lond) 95:707–735
  • Zenk MH (1978) The impact of cell cultures on industry. In: Thorpe TA (ed) Frontiers of plant tissue culture. University of Calgary, Canada, pp 1–13

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