Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma

• Autorzy: Liu J. , Yang C. , Gu Y. , Li C. , Zhang H. , Zhang W. , Zheng C.
• Języki publikacji: EN

Abstrakty

EN

Background: Epstein–Barr virus (EBV) infection is causatively associated with a variety of human cancers, including gastric cancer (GC), which has one of the highest mortality rates of all human cancers. Long non-coding RNAs (lncRNAs) show important regulatory roles in human GC. SNHG8 is a recently identified lncRNA that was reported to show abnormal expression pattern in GC. However, little is known of its biological function in EBV-associated GC. Methods: We used cell viability, colony formation and cell cycle assays to investigate the roles of lncRNA SNHG8 in the cell growth of EBV-associated GC. Results: The transcript levels of SNHG8 in the cultured EBV-associated GC cells were significantly higher in the cultured EBV-associated GC cells compared with the levels in normal human gastric mucosal cells and EBV-negative GC cells. Knockdown of SNHG8 with specific shRNAs inhibited cell proliferation and colony formation and arrested the cell cycle in the G0/G1 phase in vitro. We also found that knockdown of SNHG8 suppressed tumor growth in vivo. Conclusions: These data indicate the pro-oncogenic potential of SNHG8 in EBVassociated GC, meaning it is a latent therapeutic target for the treatment of this type of cancer

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2018
• Tom: 23
• Opis fizyczny: p.1-10,fig.,ref.

Twórcy

autor

Liu J.

• Department of Nephology, Zibo, China

autor

Yang C.

• Department of Nephology, Zibo, China

autor

Gu Y.

• Department of Nephology, Zibo, China

autor

Li C.

• Department of Nephology, Zibo, Chin
• Department of Gastrointestinal Surgery, Zibo, China

autor

Zhang H.

• Department of Nephology, Zibo, China

autor

Zhang W.

autor

Zheng C.

• Department of Medical Care, Zibo Central Hospital, 54 Gongqingtuan Rd, Zibo, Shandong 255036, People’s Republic of China

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Identyfikatory

DOI

10.1186/s11658-018-0070-8