A rapid and simple method for detection of type II restriction endonucleases in cells of bacteria with high activity of nonspecific nucleases

• Autorzy: Podgorska B. , Kujawska G. , Skurzewski M. , Batsko O. , Kaczorowski T.
• Języki publikacji: EN

Abstrakty

EN

 In this work we describe a novel, rapid and simple microscale procedure for identification of restriction endonuclease activity in bacteria lysates, which contain high levels of non-specific DNA nucleases.

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2012
• Tom: 59
• Numer: 4
• Opis fizyczny: p.669-672,fig.,ref.

Twórcy

autor

Podgorska B.

• Department of Molecular Evolution, University of Gdańsk, Gdańsk, Poland
• Department of Microbiology, University of Gdańsk, Gdańsk, Poland

autor

Kujawska G.

• Department of Microbiology, University of Gdańsk, Gdańsk, Poland

autor

Skurzewski M.

• Department of Microbiology, University of Gdańsk, Gdańsk, Poland

autor

Batsko O.

• Department of Microbiology, University of Gdańsk, Gdańsk, Poland

autor

Kaczorowski T.

• Department of Microbiology, University of Gdańsk, Gdańsk, Poland

Bibliografia

• Arber W (1979) Promotion and limitation of genetic exchange. Science 205: 361-365. 
• Belavin PA, Dedkov VS, Degtiarev SKh (1988) A simple technique for detection of restriction endonucleases in bacterial colonies. Appl Biochem Microbiol (Russia) 24: 121-124. 
• Bickle TA, Krüger DH (1993) Biology of DNA restriction. Microbiol Rev 57: 434-450. 
• Edberg SC, Clare D, Moore MH, Singer JM (1979) Rapid identification of Enterobacteriaceae from blood cultures with the Micro-ID system. J Clin Microbiol 10: 693-697. 
• Edwards PR and Ewing W H (1972) Identification of Enterobacteriaceae. Burgess, Minneapolis 3rd edN.
• Greene PJ, Heyneker HL, Bolivar F, Rodriguez RL, Betlach MC, Covarrubias AA, Backman K, Russel DJ, Tait R, Boyer HW (1978) A general method for purification of restriction enzymes. Nucleic Acids Res 5: 2373-2380. 
• Isenberg HD (2007) Clinical microbiology procedures handbook. Washington DC: Am Soci for Microbiol 2nd edn.
• Kaida T, Ginting L, Kanda K, Murata A, Kato F (1999) Screening of type II restriction endonuclease from soil bacteria. Bull Fac Agr 84: 1-9.
• Krüger DH, Bickle A (1983) Bacteriophage survival: multiple mechanisms for avoiding the deoxyribonucleic acid restriction systems of their hosts. Microbiol Rev 47: 345-360. 
• Mruk I, Kaczorowski T (2003) Genetic organization and molecular analysis of the EcoVIII restriction-modification system of Escherichia coli E1585-68 and its comparison with isospecific homologs. Appl Environ Microbiol 69: 2638-2650. 
• Pingoud A, Jeltsch A (2001) Structure and function of type II restriction endonucleases. Nucl Acids Res 29: 3705-3727. 
• Poch MT, Somkuti GA (1993) Rapid screening of lactic acid bacteria for endonuclease activity. Biotechnol Tech 7: 781-784.
• Poch MT, Somkuti GA (1995) Isolation of SagI, a new HaeIII isoschizomer from Streptococcus agalactiae. Appl Microbiol Biotechnol 43: 282-284. 
• Puchkova LI, Ushakova TA, Mikhailova VK, Serov GD, Krivopalova GN, Repin VE (2002) Testing and isolation of high-purity restriction endonucleases. Appl Biochem Microbiol 38: 20-24. 
• REBASE http://rebase.neb.com
• Roberts RJ (2005) How restriction enzymes became the workhorses of molecular biology. Proc Natl Acad Sci 102: 5905-5908. 
• Sambrook J, Fritsch E.T, Maniatis T (1989) Molecular Cloning: A laboratory manual. Cold Spring Harbor Laboratory Pr; 2nd edn.
• Smith DI, Blattner FR, Davies J (1976) The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii. Nucleic Acids Res 3: 343-353. 
• Whitehead PR, Brown NL (1985) A simple and rapid method for screening bacteria for type II restriction endonucleases: enzymes in Aphanothece halophytica. Arch Microbiol 141: 70-74. 

Uwagi

rekord w opracowaniu