Properties of single noninactivating K plus channel currents in medial prefrontal cortex (mPFC) pyramidal neurons

• Autorzy: Ksiazek A. , Ladno V. , Witkowski G. , Szulczyk P.
• Języki publikacji: EN

Abstrakty

EN

Distorted activity of mPFC neurons occurs in age-dependent, widespread neuropsychiatric disorders. Single noninactivating K+ channel currents were recorded in cell attached configuration from freshly dispersed mPFC pyramidal neurons. Neurons were obtained from young (20 days old, 185 channels analyzed), periadolescent (40 days old, 71 channels analyzed) and adult rats (60 days old, 84 channels analyzed). There were found 3 major groups of channel currentsin tested neurons: (1) Ca++ dependent K+ channel currents; these channel currents when recorded in cell attached configuration expressed only outward conductance (150 pS); in inside out configuration they expressed also inward conductance when Ca++ ions applied to the cytoplasmatic side of the membrane; (2) large conductance leak channel currents showed outward (150 pS) and inward (150 pS) conductance in cell attached configuration; these channels were strongly mechanosensitive; (3) small conductance leak channel currents had outward and inward conductance about 30–40 pS. Each category of tested channel currents had similar properties biophysical properties in neurons obtained from rats of different age. Smaller proportion of Ca++ dependent K+ channel currents and larger proportion large conductance leak channel currents were found in pyramidal neurons obtained from preadolescent rats comparing to young and adult rats (see table). Supported by NN401584638 and NN401076537.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2012
• Tom: 72
• Numer: 2
• Opis fizyczny: p.199

Twórcy

autor

Ksiazek A.

• Department of Physiology and Pathophysiology, Medical University of Warsaw, Warsaw, Poland

autor

Ladno V.

• Department of Physiology and Pathophysiology, Medical University of Warsaw, Warsaw, Poland

autor

Witkowski G.

• Department of Physiology and Pathophysiology, Medical University of Warsaw, Warsaw, Poland

autor

Szulczyk P.

Uwagi

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