Characterization of Clinostomum metacercariae using microscopic and molecular approaches

• Autorzy: Zimik P. , Sharma S. , Roy B.
• Języki publikacji: EN

Abstrakty

EN

One of the fundamental aspects in understanding the biology, diversity and epidemiology of a parasite lies in its proper identification. In the present study, morphological and molecular characterization of Clinostomum metacercariae recovered from an ornamental fish, Trichogaster fasciata, was carried out in order to ascertain its identity. To serve the purpose, scanning electron micrographs and gene sequences for two commonly used molecular markers, i.e., nuclear ribosomal internal transcribed spacer 2 (rDNA-ITS2) and mitochondrial cytochrome c oxidase subunit 1 (mtCO1) were obtained. The sequences were further used for generating similarity index matrix as well as inferring phylogenies. Light and electron microscopic observations on metacercariae of the parasite revealed that it belongs to the genus Clinostomum. Identification of the same up to the level of species was made possible through sequence and phylogenetic analyses. The ITS2 sequence analyses of our species (KX758630) showed similarity to unidentified Clinostomum sp. reported from Nigeria (KY865625) and China (KP110579), and C. tilapiae recorded from South Africa (KX034048) and Nigeria (KY649353). However, the CO1 gene analyses suggested it to be highly identical to C. philippinense and the same was also corroborated in the phylogenetic analysis. Thus, morphological and molecular characterization revealed that the recovered metacercariae belong to the species C. philippinense. Additionally, a brief description of secondary structures of ITS2 of various species of Clinostomum has also been discussed.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2019
• Tom: 65
• Numer: 1
• Opis fizyczny: p.87-97,fig.,ref.

Twórcy

autor

Zimik P.

• Department of Zoology, North-Eastern Hill University, Shillong 793022, Meghalaya, India

autor

Sharma S.

• Department of Zoology, North-Eastern Hill University, Shillong 793022, Meghalaya, India

autor

Roy B.

• Department of Zoology, North-Eastern Hill University, Shillong 793022, Meghalaya, India

Bibliografia

• [1] Dias M.L.G.G., Eiras J.C., Machado M.H., Souza G.T.R., Pavanelli G.C. 2003. The life cycle of Clinostomum complanatum Rudolphi, 1814 (Digenea, Clinostomidae) on the floodplain of the high Parana´ river, Brazil. Parasitology Research 89: 506-508. doi:10.1007/s00436-002-0796-z
• [2] Hara H., Miyauchi Y., Tahara S., Yamashita H. 2014. Human laryngitis caused by Clinostomum complanatum. Nagoya Journal Medical Science 76: 181-185.
• [3] Tiewchaloern S., Udomkijdecha S., Suvouttho S., Chunchamsri K., Waikagul J. 1999. Clinostomum trematode from human eye. Southeast Asian Journal of Tropical Medicine and Public Health 30: 2.
• [4] Park C.W., Kim J.S., Joo H.S., Kim J. 2009. A human case of Clinostomum complanatum infection in Korea. Korean Journal of Parasitology 47: 401-404. https://doi.org/10.3347/kjp.2009.47.4.401
• [5] Shareef P.A.A., Abidi S.M.A. 2012. Incidence and histopathology of encysted progenetic metacercaria of Clinostomum complanatum (Digenea: Clinostomidae) in Channa punctatus and its development in experimental host. Asian Pacific Journal of Tropical Biomedicine 2: 421-426. https://dx.doi.org/10.1016/S2221-1691(12)60068-9
• [6] Ukoli F.M.A. 1966. On Clinostomum tilapiae n. sp. and C. phalacrocoracis Dubois, 1931 from Ghana, and a discussion of the systematics of the genus Clinostomum Leidy, 1856. Journal of Helmintholoy 40: 187-214.
• [7] Yamaguti S. 1971. Synopsis of digenetic trematodes of vertebrates Vol. 1. Tokyo, Keigaku Publishing Co.
• [8] Feizullaev N.A., Mirzoeva S.S. 1983. Revision of the superfamily Clinostomoidea and analysis of its system. Parazitologiya 17: 3-11.
• [9] Gustinelli A., Caffara M., Florio D., Otachi E.O., Wathuta E.M., Fioravanti M.L. 2010. First description of the adult stage of Clinostomum cutaneum Paperna, 1964 (Digenea: Clinostomidae) from grey herons Ardea cinerea L. and a redescription of the metacercaria from the Nile tilapia Oreochromis niloticus niloticus (L.) in Kenya. Systematic Parasitology 76: 39-51. https://doi.org/10.1007/s11230-010-9231-5
• [10] Matthews D., Cribb T.H. 1998. Digenetic trematodes of the genus Clinostomum Leidy, 1856 (Digenea: Clinostomidae) from birds of Queensland, Australia, including C. wilsoni n. sp. from Egretta intermedia. Systematic Parasitology 39: 199-208. https://doi.org/10.1023/A:1005982530560
• [11] Nolan M.J., Cribb T.H. 2005. The use and implications of ribosomal DNA sequencing for the discrimination of digenean species. Advances in Parasitology 60: 102-163. https://doi.org/10.1016/S0065-308X(05)60002-4
• [12] Ghatani S., Shylla J.A., Tandon V., Chatterjee A., Roy B. 2012. Molecular characterization of pouched amphistome parasites (Trematoda: Gastrothylacidae) using ribosomal ITS2 sequence and secondary structures. Journal of Helminthology 86: 117-124. https://doi.org/10.1017/S0022149X11000125
• [13] Briosio-Aguilar R., García-Varela M., Hernández-Mena D.I., Rubio-Godoy M., Pérez-Ponce de León G. 2018. Morphological and molecular characterization of an enigmatic clinostomid trematode (Digenea: Clinostomidae) parasitic as metacercariae in the body cavity of freshwater fishes (Cichlidae) across Middle America. Journal of Helminthology 11: 1-14. https://doi.org/10.1017/S0022149X18000445
• [14] Dzikowski R., Levy M.G., Poore M.F., Flowers J.R., Paperna I. 2004. Clinostomum complanatum and Clinostomum marginatum (Rudolphi, 1819) (Digenea: Clinostomidae) are separate species based on differences in ribosomal DNA. Journal of Parasitology 90: 413-414. https://doi.org/10.1645/GE-159R
• [15] Caffara M., Locke S.A., Gustinelli A., Marcogliese D.J., Fioravanti M.L. 2011. Morphological and molecular differentiation of Clinostomum complanatum and Clinostomum marginatum (Digenea: Clinostomidae) metacercariae and adults. Journal of Parasitology 97: 884-891. https://doi.org/10.1645/GE-2781.1
• [16] Sereno-Uribe A.L., Pinacho P.C.D., Varela M.G., Leon G.P.P.D. 2013. Using mitochondrial and ribosomal DNA sequences to test the taxonomic validity of Clinostomum complanatum Rudolphi, 1814 in fish-eating birds and freshwater fishes in Mexico, with the description of a new species. Parasitology Research 112: 2855-2870. doi:10.1007/s00436-013-3457-5
• [17] Rosser T.G., Baumgartner W.A., Alberson N.R., Woodyard E.T., Reichley S.R., Wise D.J., Pote L.M., Griffin M.J. 2016. Austrodiplostomum sp., Bolbophorus sp. (Digenea: Diplostomidae), and Clinostomum marginatum (Digenea: Clinostomidae) metacercariae in inland silverside Menidia beryllina from catfish aquaculture ponds, with notes on the infectivity of Austrodiplostomum sp. cercariae in channel catfish Ictalurus punctatus. Parasitology Resesrch 115: 4365-4378. https://doi.org/10.1007/s00436-016-5222-z
• [18] Athokpam V.D., Tandon V. 2014. Morphological and molecular characterization of Posthodiplo stomum sp. (Digenea: Diplostomidae) metacercaria in the muscles of snakeheads (Channa punctata) from Manipur, India. Helminthology 51: 141-152. https://doi.org/10.2478/s11687-014-0221-z
• [19] Pekmezci G.Z., Onuk E.E., Bolukbas C.S., Yardimci B., Gurler A.T., Acici M., Umur S. 2014. Molecular identification of Anisakis species (Nematoda: Anisakidae) from marine fishes collected in Turkish waters. Veterinary Parasitology 201: 82-94. https://doi.org/10.1016/j.vetpar.2014.01.005
• [20] Sharma S., Lyngdoh D., Roy B., Tandon V. 2016. Differential diagnosis and molecular characterization of Hymenolepis nana and Hymenolepis diminuta (Cestoda: Cyclophyllidea: Hymenolepididae) based on nuclear rDNA ITS2 gene marker. Parasitology Research 115: 4293-4298. https://doi.org/10.1007/s00436-016-5210-3
• [21] Lyngdoh D., Sharma S., Roy B., Tandon V. 2016. Animal Fascioliasis: Perspectives from high altitudinal regions. Veterinary Pararasitoly 232: 21-31. https://doi.org/10.1016/j.vetpar.2016.11.007
• [22] Kalyanasundaram A., Blanchard K.R., Kendall R.J. 2017. Molecular identification and characterization of partial COX1 gene from caecal worm (Aulonocephalus pennula) in Northern bobwhite (Colinus virginianus) from the Rolling Plains Ecoregion of Texas. International Journal of Parasitology: Parasites and Wildlife 6: 195-201. https://doi.org/10.1016/j.ijppaw.2017.07.002
• [23] Coleman A.W. 2003. ITS2 is a double-edged tool for eukaryotic evolutionary comparisons. Trends in Genetics 19: 370-375. https://doi.org/10.1016/S0168-9525(03)00118-5
• [24] Coleman A.W. 2007. Pan-eukaryote ITS2 homologies revealed by RNA secondary structure. Nucleic Acids Research 35: 3322-3329. https://doi.org/10.1093/nar/gkm233
• [25] Prasad P.K., Goswami L.M., Tandon V., Chatterjee A. 2011. PCR-based molecular characterization and insilico analysis of food-borne trematode parasites Paragonimus westermani, Fasciolopsis buski and Fasciola gigantica from northeast India using ITS2 rDNA. Bioinformation 6: 64-68.
• [26] Rampersad S.N. 2014. ITS1, 5.8S and ITS2 secondary structure modeling for intra-specific differentiation among species of the Colletotrichum gloeosporioides sensu lato species complex. Springerplus 3: 684. https://doi.org/10.1186/2193-1801-3-684
• [27] Zhang W., Yuan Y., Shuo Y., Jianjun H., Luqi H. 2015. ITS2 secondary structure improves discrimination between medicinal “Mu Tong” species when using DNA barcoding. PLoS One 1: 10. https://doi.org/10.1371/journal.pone.0131185
• [28] Roy B., Tandon V. 1991. Usefulness of tetramethylsilane in the preparation of helminth parasites for scanning electron microscopy. Rivista di Para ssi to logia 8: 207-215.
• [29] Sambrook J., Russell D.W. 2001. Preparation and analyses of eukaryotic genomic DNA. In: Molecular cloning: a laboratory manual. Vol. 1, 3rd ed. Cold Spring Harbor Laboratory Press, New York: 51-54.
• [30] Bowles J., Blair D., McManus D.P. 1995. A molecular phylogeny of the human schistosomes. Molecular Phylogenetics and Evolution 4: 103-109. https://doi.org/10.1006/mpev. 1995.1011
• [31] Steenkiste N.V., Locke S.A., Castelin M., Marcogliese D.J. Abbott C.L. 2015. New primers for DNA barcoding of digeneans and cestodes (Platyhelminthes). Molecular Ecology Resource 15: 945-952. doi:10.1111/1755-0998.12358
• [32] Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30: 2725-2729. https://doi.org/10.1093/molbev/mst197
• [33] Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium Series 41. Oxford University Press: 95-98.
• [34] Ronquist F., Teslenko M., van der Mark P., Ayres D.L., Darling A., Höhna S., Larget B., Liu L., Suchard M.A., Huelsenbeck J.P. 2012. MrBayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539-542. https://doi.org/10.1093/sysbio/sys029
• [35] Eddy S. 1998. Profile hidden Markov models. Bioinformatics 14: 755-763.
• [36] Gruber A.R., Lorenz R., Bernhart S.H., Neubock R., Hofacker I.L. 2008. The Vienna RNA web suite. Nucleic Acids Research 36: 70-74. https://doi.org/ 10.1093/nar/gkn188
• [37] Rosser T.G., Alberson N.R., Woodyard E.T., Cunninghamm F.L., Pote L.M., Griffin M.J. 2017. Clinostomum album n. sp. and Clinostomum marginatum (Rudolphi, 1819), parasites of the great egret Ardea alba L. from Mississippi, USA. Systematic Parasitology 94: 35-49. https://doi.org/10.1007/s11230-016-9686-0
• [38] Acosta A.A., Caffara M., Fioravanti M.L., Utsunomia R., Zago A.C., Franceschini L., da Silva R.J. 2016. Morphological and molecular characterization of Clinostomum detruncatum (Trematoda: Clinostomidae) metacercariae infecting Synbranchus marmoratus. Journal of Parasitology 102: 151-156. https://doi.org/10.1645/15-773
• [39] Abidi S.M.A., Ahmad M., Nizami W.A., Hanna R.E.B. 1988. Clinostomum complanatum: Tegumental surface changes during in vivo development. International Journal of Parasitology 18: 433-439.
• [40] Ngamniyom A., Manaporn M., Panyarachun B. 2012. Thai Medaka, Oryzias minutillus Smith, 1945 (Beloniformes: Adrianichthyidae): a new host species of Clinostomum complanatummetacercariae (Digenea: Clinostomatidea) and the surface topography by using SEM. Chiang Mai Journal of Science 39: 540-544. http://www.thaiscience.info/journals/Article/CMJS/ 10905272.pdf
• [41] Rizvi A., Alam M. Md., Parveen S., Saleemuddin M. Abidi S.M.A. 2012. Abandoning the ship: spontaneous mass exodus of Clinostomum complanatum (Rudolphi, 1814) progenetic metecercariae from the dying intermediate host Trichogaster fasciatus (Bloch & Schneider, 1801). Journal of Parasitic Diseases 36: 139-140. doi:10.1007/s12639-011-0077-9
• [42] Yooyen T., Wongsawad C., Kumchoo K., Chaiyapo M. 2006. A new record of Clinostomum philippinensis (Valasquez, 1959) in Trichogaster microlepis (Günther, 1861) from Bung Borapet, Nakhon Sawan, Thailand. Southeast Asian Journal of Tropical Medicine and Public Health 37: 3.
• [43] Perez-Ponce de Leon G., Garcia-Varela M., Pinacho-Pinacho C.D., Sereno-Uribe A.L., Poulin R. 2016. Species delimitation in trematode using DNA sequences: Middle-American Clinostomum as a case study. Parasitology 143: 1773-1789. https://doi.org/10.1017/S0031182016001517
• [44] Keller A., Förster F., Müller T., Dandekar T., Schultz J., Wolf M. 2010. Including RNA secondary structures improves accuracy and robustness in reconstruction of phylogenetic trees. Biology Direct 5: 4. https://doi.org/10.1186/1745-6150-5-4
• [45] Michot B., Despres L., Bonhomme F., Bachellerie JP. 1993. Conserved secondary structures in the ITS2 of trematode pre–rRNA. FEBS Letters 316: 247-252.
• [46] Ma Hongwei., Overstreet RM., Subbotin SA. 2008. ITS2 secondary structure and phylogeny of cystforming nematodes of the genus Heterodera (Tylenchida: Heteroderidae). Organisms, Diversity and Evolution 8: 182-193. doi:10.1016/j.ode.2007.09.001

Identyfikatory

DOI

10.17420/ap6501.187