Serum cytokines and activation ex vivo of CD4plus and CD8plus T cells in chagasic chronic Mexican patients

• Autorzy: Cruz-Robles D. , Vargas-Alarcon G. , Ortiz-Muniz R. , Reyes P.A. , Monteon V.M.
• Języki publikacji: EN

Abstrakty

EN

The clinical manifestations of human Chagas disease are associated with several factors, including immunological alterations, in this regard, many studies propose that tissue damage might be more severe in the absence of immune regulatory mechanisms, other factors are the genetic background of host and parasite. Trypanosoma cruzi population is genetically, biochemistry and pathogenic diverse along the Latin-America continent and phylogenetic ally are divided into six intra-species lineages TcI-VI. The TcI lineage has a wide distribution with heterogeneous virulence and pathogenesis within strains. In Mexico, the main circulating lineage is TcI in human infections. We analyzed intracytoplasmic cytokines of unstimulated peripheral T lymphocytes, and the level of cytokines (IL-2, IL-4, IL-12, IL-10, IFN-γ and sIL-2R) in the serum of Mexican chagasic subjects. The population studied consisted of 15 asymptomatic individuals, 17 patients with chronic chagasic cardiopathy (CCC), 20 patients with cardiopathy but negative serology for T. cruzi, and 10 healthy subjects. The analysis of CD4+ cells revealed that CCC and asymptomatic patients have higher CD25+ and CD69 activation markers than controls. The Th1 subset (CD4+/IFN- γ +) was higher in CCC than in asymptomatic and control subjects, whereas Th2 subset was markedly high in asymptomatic subjects. Circulating cytokines were below level detection with the exception of IL-2 and sIL-2R. Infection with Mexican Trypanosoma cruzi strains in asymptomatic chagasic subjects have a tendency for a Th2 response with higher CD8+/IFN-γ T cells. In contrast, CCC patients have low levels of intracellular IFN- γ and IL-2 cytokines. In both groups circulating serum cytokines are below the detectable level.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2017
• Tom: 63
• Numer: 4
• Opis fizyczny: p.299-308,fig.,ref.

Twórcy

autor

Cruz-Robles D.

• Department of Molecular Biology, Instituto Nacional de Cardiología - Ignacio Chavez, Juan Badiano 1, 14080 Mexico, Mexico

autor

Vargas-Alarcon G.

• Department of Molecular Biology, Instituto Nacional de Cardiología - Ignacio Chavez, Juan Badiano 1, 14080 Mexico, Mexico

autor

Ortiz-Muniz R.

• Department of Cellular and Molecular Biology, Universidad Autonoma Metropolitana - Unidad Iztapalapa, San Rafael Atlixco No.186, Col.Vicentina, Iztapalapa, 09340 Mexico, Mexico

autor

Reyes P.A.

• Department of Molecular Biology, Instituto Nacional de Cardiología - Ignacio Chavez, Juan Badiano 1, 14080 Mexico, Mexico

autor

Monteon V.M.

• Centro Investigaciones Biomedicas, Universidad Autonoma Campeche, Patricio Trueba s/n, Campeche 4090, Mexico

Bibliografia

• [1] Macedo A.M., Oliveira R.P., Pena S.D.J. 2002. Chagas disease: role of parasite genetic variation in pathogenesis. Expert Reviews in Molecular Medicine 4: 1-16. https://doi.org/10.1017/s1462399402004118
• [2] Marcon G.E.B., de Albuquerque D.M., Batista A.M., Andrade P.D., Almeida E.A., Guariento M.E., Teixeira M.A.B., Costa S.C.B. 2011. Trypanosoma cruzi: parasite persistence in tissues in chronic chagasic Brazilian patients. Memórias do Instituto Oswaldo Cruz 106: 85-91. doi:10.1590/s0074-02762011000100014
• [3] Cunha-Neto E., Rizzo L.V., Albuquerque F., Abel L., Guilherme L., Bocchi E., Bacal F., Carrara D., Ianni B., Mady C., Kalil J. 1998. Cytokine production profile of heart-infiltrating T cells in Chagas’ disease cardiomyopathy. Brazilian Journal of Medical and Biological Research 31: 133-137. http://dx.doi.org/10.1590/s0100-879x19980001000 18
• [4] Dutra W.O., Martins-Filho O.A., Cançado J.R., Pinto-Dias J.C., Brener Z., Gazzinelli G., Carvalho J.F., Colley D.G. 1996. Chagasic patients lack CD28 expression on many of their circulating T lymphocytes. Scandinavian Journal of Immunology 43: 88-93. doi:10.1046/j.1365-3083.1996.d01-9.x
• [5] Vitelli-Avelar D.M., Sathler-Avelar R., Dias J.C.P., Pascoal V.P.M., Teixeira-Carvalho A., Lage P.S., Elói-Santos S.M., Corrêa-Oliveira R., Martins-Filho O.A. 2005. Chagasic patients with indeterminate clinical form of the disease have high frequencies of circulating CD3+CD16-CD56+ natural killer T cells and CD4+CD25high regulatory T lymphocytes. Scandinavian Journal of Immunology 62: 297-308. doi:10.1111/j.1365-3083.2005.01668.x
• [6] Gomes J.A.S., Bahia-Oliveira L.M.G., Rocha M.O.C., Martins-Filho O.A.G., Gazzinelli G., Correa-Oliveira R. 2003. Evidence that development of severe cardiomyopathy in human Chagas’ disease is due to a Th1-specific immune response. Infection and Immunity 71: 1185-1193. doi:10.1128/iai.71.3.1185-1193.2003
• [7] Moretti E., Basso B., Cervetta L., Brigada A., Barbieri G. 2002. Patterns of cytokines and soluble cellular receptors in the sera of children with acute Chagas’ disease. Clinical and Diagnostic Laboratory Immunology 9: 1324-1327. doi:10.1128/cdli.9.6.1324-1327.2002
• [8] Cuna W.R., Choque A.G.H., Passera R., Rodriguez C. 2009. Pro-inflammatory cytokine production in chagasic mothers and their uninfected newborns. Journal of Parasitology 95: 891-894. https://doi.org/10.1645/ge-1927.1
• [9] Ward L.S., Guariento M.E., Fernandes G.A., Maciel R.M.B. 1999. Serum cytokines in chronic Chagas’ disease. Revista da Sociedade Brasileira de Medicina Tropical 32: 285-289. http://dx.doi.org/10.1590/s0037-868219990003000 10
• [10] dos Santos D.M., Talvani A., da Mata Guedes P.M., Machado-Coelho G.L.L., de Lana M., Bahia M.T. 2009. Trypansoma cruzi: genetic diversity influences the profile of immunoglobulins during experimental infection. Experimental Parasitology 121: 8-14. https://doi.org/10.1016/j.exppara.2008.09.012
• [11] Zingales B., Andrade S.G., Briones M.R.S., Campbell D.A., Chiari E., Fernandes O., Guhl F., Lages-Silva E.,Macedo A.M.,Machado C.R.,Miles M.A., Romanha A.J, Sturm N.R., Tibayrenc M., Schijman A.G. 2009. A new consensus for Trypanosoma cruzi intraspecific nomenclature: second revision meeting recommends TcI to TcVI. Memórias do Instituto Oswaldo Cruz 104: 1051-1054. http://dx.doi.org/10.1590/s0074-02762009000700021
• [12] Bosseno M.-F., Barnabé C., Gastélum E.M., Kasten F.L., Ramsey J., Espinoza B., Brenière S.F. 2002. Predominance of Trypanosoma cruzi lineage I in Mexico. Journal of Clinical Microbiology 40: 627-632. doi:10.1128/jcm.40.2.627-632.2002
• [13] Ruíz-Sanchez R., de León M.P., Matta V., Reyes P.A. López R., Jay D., Monteón V.M. 2005. Trypanosoma cruzi isolates from Mexican and Guatemalan acute and chronic chagasic cardiopathy patients belong to Trypanosoma cruzi I. Memórias do Instituto Oswaldo Cruz 100: 281-283. http://dx.doi.org/10.1590/s0074-0276200500030001 2
• [14] Pérez-Fuentes R., Torres-Rasgado E., Salgado-Rosas H., Zamora-Ginez I., Sánchez-Guillén M.C. 2008. The anti-oxidant defence response in individuals with the indeterminate form of Chagas disease (American trypanosomiasis). Annals of Tropical Medicine and Parasitology 102: 189-197. http://dx.doi.org/10.1179/ 136485908x267858
• [15] Monteón V.M., Hernández O., López R., Reyes P.A. 2009. Cytokine expression at the inoculation site and nearby tissues in an animal model infected with metacyclic trypomastigotes of Trypanosoma cruzi. Tropical Medicine and Health 37: 141-147. http://doi.org/10.2149/tmh.2009-03
• [16] Espinoza B., Rico T., Sosa S., Oaxaca E., Vizcaino-Castillo A., Caballero M.L., Martínez I. 2010. Mexican Trypanosoma cruzi TCI strains with different degrees of virulence induce diverse humoral and cellular immune responses in a murine experimental infection model. Journal of Biomedicine and Biotechnology 2010: 890672. doi:10.1155/2010/890672
• [17] Monteón V., Quen-Rámirez E., Macedo-Reyes V., Lopez R., Acosta-Viana K., Pennigton P., Ramos-Ligonio A. 2016. Pre-exposure to faeces or saliva of Triatoma dimidiata decreases parasitemia in mice challenged with Trypanosoma cruzi: a description of the inflammatory reaction at the inoculation site. Annals of Parasitology 62: 209-219. doi:10.17420/ap6203.54
• [18] Lorena V.M.B., Lorena I.M.B., Braz S.C.M., Melo A.S., Melo M.F.A.D.,Melo M.G.A.C., Silva E.D., Ferreira A.G.P., Morais C.N.L., Costa V.M.A., Correa-Oliveira R., Gomes Y.M. 2010. Cytokine levels in serious cardiopathy of Chagas disease after in vitro stimulation with recombinant antigens from Trypanosoma cruzi. Scandinavian Journal of Immunology 72: 529-539. doi:10.1111/j.1365-3083.2010.02462.x
• [19] Laucella S.A., Pérez Mazliah D., Bertocchi G., Alvarez M.G., Cooley G., Viotti R., Albareda M.C., Lo coco B., Postan M., Armenti A., Tarleton R.L. 2009. Changes in Trypanosoma cruzi-specific immune responses after treatment: surrogate markers of treatment efficacy. Clinical Infectious Diseases 49: 1675-1684. https://doi.org/10.1086/648072
• [20] Alvarez M.G., Postan M., Weatherly D.B., Albareda M.C., Sidney J., Sette A., Olivera C., Armenti A.H., Tarleton R.L., Laucella S.A. 2008. HLA class IT cell epitopes from trans-sialidase proteins reveal functionally distinct subsets of CD8+ T cells in chronic Chagas disease. PLOS Neglected Tropical Diseases 2: e288. doi:10.1371/journal.pntd.0000288
• [21] Martín-Romero C., Santos-Alvarez J., Goberna R., Sánchez-Margalet V. 2000. Human leptin enhances activation and proliferation of human circulating T lymphocytes. Cell Immunology 199: 15-24. https://doi.org/10.1006/cimm.1999.1594
• [22] Bixby L.M., Tarleton R.L. 2008. Stable CD8+ T cell memory during persistent Trypanosma cruzi infection. Journal of Immunology 181: 2644-2650. https://doi.org/10.4049/jimmunol.181.4.2644
• [23] Lanzavecchia A., Sallusto F. 2000. Dynamics of T lymphocyte responses: intermediates, effectors, and memory cells. Science 290: 92-97. doi:10.1126/science.290.5489.92
• [24] de Meis J., Morrot A., Farias-de-Oliveira D.A., Villa-Verde D.M.S., Savino W. 2009. Differential regional immune response in Chagas disease. PLOS Neglected Tropical Diseases 3: e417. doi:10.1371/journal.pntd.0000417
• [25] Donohue J.H., Rosenberg S.A. 1983. The fate of interleukin-2 after in vivo administration. Journal of Immunology 130: 2203-2208.
• [26] Gonçalves Souza V.C., dos Santos J.T., Cabral F.L., Barbisan F., Azevedo M.I., Dias Carli L.F., de Avila Botton S., dos Santos Jaques J.A., Rosa Leal D.B. 2017. Evaluation of P2X7 receptor expression in peripheral lymphocytes and immune profile from patients with indeterminate form of Chagas disease. Microbial Pathogenesis 104: 32-38. doi:10.1016/j.micpath.2017.01.002
• [27] Sousa G.R., Gomes J.A.S., Gomes Fares R.C., de Souza Damásio M.P., Chaves A.T., Ferreira K.S., Pereira Nunes M.C., Medeiros N.I., Azevedo Valente V.A., Corrêa-Oliveira R., da Costa Rocha M.O. 2014. Plasma cytokine expression is associated with cardiac morbidity in Chagas disease. PLoS ONE 9: e87082. doi:10.1371/journal.pone.0087082
• [28] Rodríguez-Angulo H., Marques J., Mendoza I., Villegas M., Mijares A., Gironès N., Fresno M. 2017. Differential cytokine profiling in Chagasic patients according to their arrhythmogenic-status. BMC Infectious Diseases 17: 221. doi:10.1186/s12879-017-2324-x
• [29] Cartier A., Côté M., Lemieux I., Pérusse L., Tremblay A., Bouchard C., Després J.-P. 2009. Sex differences in inflammatory markers: what is the contribution of visceral adiposity? American Journal of Clinical Nutrition 89: 1307-1314. doi:10.3945/ajcn.2008.27030
• [30] Heinisch R.H., Zanetti C.R., Comin F., Fernandes J.L., Ramires J.A., Serrano Jr.C.V. 2005. Serial changes in plasma levels of cytokines in patients with coronary artery disease. Vascular Health and Risk Management 1: 245-250.
• [31] Milner T., Reilly L., Nausch N., Midzi N., Mduluza T., Maizels R., Mutapi F. 2010. Circulating cytokine levels and antibody responses to human Schistosoma haematobium: IL-5 and IL-10 levels depend upon age and infection status. Parasite Immunology 32: 710-721. doi:10.1111/j.1365-3024.2010.01235.x

Identyfikatory

DOI

10.17420/ap6304.116