EN
Tomato black ring virus (TBRV) is a serious pathogen of many plants worldwide. TBRV has single stranded RNA genomes divided into two genomic RNAs. In Institute of Plant Protection – National Research Institute we collected different isolates of TBRV from zucchini, cucumber, tomato, potato, Robinia pseudoaccacia L. and Sambucus nigra. Using ELISA test and Dot blot immunoassay we were able to distinguish two serologically related viruses (TBRV and Beet ringspot virus). Results from serological tests were confirmed by RT-PCR. The expected product about 1200 bp was amplified for all isolates tested.