In vitro efficacy of different concentrations of lupeol on old world Leishmania donovani

• Autorzy: Abbas T.F. , Ali H.Z.
• Języki publikacji: EN

Abstrakty

EN

Leishmaniosis is a tropical neglected parasitic disease that is endemic in many countries, includingMiddle East, with no existing effective vaccines. The bite of female sand-fly transmits the causative agent, Leishmaniaspp., to humans. High toxicity, resistance and treatment failure of the available chemotherapy against visceralleishmaniosis demands the investigation of new anti-leishmanial compounds. Lupeol is a form of triterpene isolatedfrom several medicinal plants and possesses an antimicrobial property. In this study, cytotoxic effect of lupeol wasscreened against the mammalian amastigotes form and insect promastigote form of Leishmania donovani,followingthree cycles of incubation at different concentrations by MTT assay. Results revealed the in vitroanti-leishmanial effectof lupeol on both forms of the parasite where significant decline in promastigotes and amastigotes growth was observed.This was conducted along three times of follow up (24, 48, 72) hours, in comparison to the classical sodiumstibogluconate treatment. Cell viability was calculated and the minimum IC50was detected after 48 hours foramastigotes and 24 hours for promastigotes, 12.125 μM, 102.78 μM, respectively. Given the severity of visceralleishmaniosis and the toxicity of conventional chemotherapies, the anti-leishmanial activity of lupeol suggested apromising compound for additional clinical trials.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2024
• Tom: 70
• Numer: 2
• Opis fizyczny: p.73-79,fig.,ref.

Twórcy

autor

Abbas T.F.

• College of Pharmacy, University of Al-Muthana, Samawa, Almuthana, Iraq

autor

Ali H.Z.

• Department of Biology, College of Science, University of Baghdad, Al-Jaderiya Campus Block A, Risafa district, Baghdad, 10071, Iraq

Identyfikatory

DOI

10.17420/ap7002.523