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2002 | 07 | 4 |
Tytuł artykułu

The role of focal adhesion kinase in the emigration of cells from confluent cultures

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
We studied the effect of the modification of focal adhesion kinase (FAK) on the growth, migration and adhesion of C3H 10T1/2 cells. Cells transfected with plasmid coding for antisense FAK displayed a low level of FAK protein. Interestingly, the transfected cells achieved a higher saturation density at confluence, and displayed reduced adhesion and enhanced emigration from a confluent layer of cells when stimulated with fibronectin. In conclusion, it can be postulated that FAK plays an important role in the mechanism of contact inhibition.
Wydawca
-
Rocznik
Tom
07
Numer
4
Opis fizyczny
p.1047-1057,fig.,ref.
Twórcy
autor
  • Department of Cell Biology, Cancer Center, M.Sklodowska-Curie Institute of Oncology, W.K.Roentgen 5, 02-781 Warsaw, Poland
  • Department of Cell Biology, Cancer Center, M.Sklodowska-Curie Institute of Oncology, W.K.Roentgen 5, 02-781 Warsaw, Poland
  • Department of Cell Biology, Cancer Center, M.Sklodowska-Curie Institute of Oncology, W.K.Roentgen 5, 02-781 Warsaw, Poland
autor
  • Department of Cell Biology, Cancer Center, M.Sklodowska-Curie Institute of Oncology, W.K.Roentgen 5, 02-781 Warsaw, Poland
  • Department of Cell Biology, Cancer Center, M.Sklodowska-Curie Institute of Oncology, W.K.Roentgen 5, 02-781 Warsaw, Poland
autor
  • Center for Biotechnology, Temple University, 1900N 12th Street, Philadelphia, USA
autor
  • Department of Cell Biology, Cancer Center, M.Sklodowska-Curie Institute of Oncology, W.K.Roentgen 5, 02-781 Warsaw, Poland
Bibliografia
  • 1. Abercrombie, M. Contact inhibition and malignancy. Nature 281 (1979) 259-262.
  • 2. Schwartz, M. A. Integrins, oncogenes, and anchorage independence. J. Cell Biol. 139 (1997) 575-578.
  • 3. Ilić, D., Damsky, C. H. and Yamamoto, T. Focal adhesion kinase: at the crossroads of signal transduction. J. Cell Sci. 110 (1997) 401-407.
  • 4. Assoian, R. K. Anchorage- dependent cell cycle progression. Mini-review J. Cell Biol. 136 (1997) 1-4.
  • 5. Reznikoff, C. A., Brankow, D. W., and Heidelberger, C. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to post-confluence inhibition of division. Cancer Res. 33 (1973) 3231-3238.
  • 6. Takeuchi, Y., Suzawa, M., Kikuchi, T., Nishida, E., Fujita, T. and Matsumoto, T. Differentiation and transforming growth factor-β receptor down-regulation by collagen-α2β1 integrin interaction is mediated by focal adhesion kinase and its downstream signals in murine osteoblastic cells. J. Biol. Chem. 272 (1997) 29309-29316.
  • 7. Willumsen, B. M., Papageorge, A. G., Kung, F. J., Bekesi, E., Robins, T., Johnsen, M., Vass, W. and Lowly, D. R. Mutational analysis of a ras catalytic domain. Mol. Cell Biol. 9 (1986) 2646-2654.
  • 8. Janik, P., Szaniawska, B., Kowalczyk, D. and Miłoszewska, J. The effect of phorbol ester treatment on migration of C3H 10T1/2 and BT5C glioma cells: possible application to carcinogenesis. J. Cancer Res. Clin. Oncol. 120 (1994) 156-158.
  • 9. Albini, A., Iwamoto, Y., Kleinman, H. K., Martin, G. R., Aaronson, S. S., Kozlowski, J. M. and McEwan, R. N. A rapid in vitro assay for quantitating the invasive potential of tumor cells. Cancer Res. 47 (1987) 3239-3245.
  • 10. Repesh, L. A. A new in vitro assay for ąuantitating tumor cell invasion. Invasion Metastasis 9 (1989) 192-208.
  • 11. Batt, D. B. and Roberts, T. M. Cell density modulates protein-tyrosine phosphorylation. J. Biol. Chem. 273 (1998) 3408-14.
  • 12. Miłoszewska, J., Trawicki, W., Janik, P., Moraczewski, J., Przybyszewska, M. and Szaniawska, B. Protein kinase C translocation in relation to proliferative status of C3H 10T1/2 cells. FEBS Lett. 206 (1986) 283-286.
  • 13. Miłoszewska, J., Trembacz, H. and Janik, P. Signal transduction in confluent C3H10T1/2 cells. The role of focal adhesion kinase. Acta Biochim. Pol. 48 (2001) 175-181.
  • 14. Viñals, F. and Pouysségeur, J. Confluence of vascular endothelial cells induces cell cycle exit by inhibiting p42/p44 mitogen-activated protein kinase activity. Mol. Cell. Biol. 9 (1999) 2763-2772.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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