Coomassie blue G250 for visualization of active bacteria from lake environment and culture

• Autorzy: Kiersztyn B. , Siuda W. , Chrost R.
• Języki publikacji: EN

Abstrakty

EN

Bacteria play a fundamental role in the cycling of nutrients in aquatic environments. A precise distinction between active and inactive bacteria is crucial for the description of this process. We have evaluated the usefulness of Coomassie Blue G250 for fluorescent staining of protein containing potentially highly active bacteria. We found that the G250 solution has excitation and emission properties appropriate for direct epifluorescence microscopy observations. It enables fast and effective fluorescent visualization of living, protein-rich bacteria, both in freshwater environment and culture. Our results revealed that the number of G250-stained bacteria from eutrophic lake was positively correlated with other standard bacterial activity markers, like number of bacteria containing 16S rRNA, bacterial secondary productionor maximal potential leucine-aminopeptidase activity. In case of the E. coli culture, the percentage of bacteria visualized with G250 was similar to that of bacteria which accumulated tetracycline. Compared to other common methods utilizing fluorogenic substances forbacteria staining, the approach we evaluated is inexpensive and less hazardous (for example mutagenic) to the environment and researchers. It can be regarded as an additional or alternative method for protein rich, active bacteria staining.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2017
• Tom: 66
• Numer: 3
• Opis fizyczny: p.365-373,fig.,ref.

Twórcy

autor

Kiersztyn B.

• Biological and Chemical Research Centre, Department of Microbial Ecology and Environmental Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland

autor

Siuda W.

• Biological and Chemical Research Centre, Department of Microbial Ecology and Environmental Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland

autor

Chrost R.

• Biological and Chemical Research Centre, Department of Microbial Ecology and Environmental Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland

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Identyfikatory

DOI

10.5604/01.3001.0010.4867