In vitro propogation of Physalis peruviana (L.) using apical shoot explants

• Autorzy: Guney M. , Kafkas S. , Kefayati S. , Motalebipour Z. , Turkeli Y. , Ercisli S. , Kafkas E.

Warianty tytułu

PL

Rozmnażanie in vitro Physalis peruviana (L.) przy użyciu eksplantów ze szczytowych pędów

• Języki publikacji: EN

Abstrakty

EN

Physalis peruviana L. is belongs to Solanaceae family and commonly known as Cape gooseberry. More recently it is very popular and widely used as medicinal plant to treat malaria, asthma, hepatitis, dermatitis and rheumatism and has diuretic and antiinflammatory properties. In this study, it was aimed to develop in vitro propagation protocol for P. peruviana L. using apical shoots as an explant sources. Regenerated plants were evaluated based on their multiplication rate and shoot length using various concentration of BAP (1, 2, 3 mg l-1) in combination with IBA (0, 0.1, 0.2, 0.4 mg l-1) and NAA (0, 0.1, 0.2, 0.4 mg l-1). In addition, efficiency of various auxin concentrations of (1 and 2 mg l-1 IBA and NAA) was also applied on root formation of P. peruviana L. The highest shoot numbers were obtained from 2 mg l-1 BAP with 0.4 mg l-1 IBA (6.00) combinations and shoot length obtained in 2 mg l-1 BAP with 0.2 mg l-1 IBA combinations (3.30 cm). As for the effects of BAP and NAA combinations; the highest shoot length were obtained from 2 mg l-1 BAP without NAA combinations (3.33 cm) while the lowest one was in 3 mg l-1 BAP with 0.4 mg/I NAA combinations. The highest root numbers were obtained from NAA application (2 mg l-1 and 1 mg l-1, respectively). In vitro derived plants were acclimatized to the soil smoothly. The present study highlights the importance of plant tissue culture in order to be used for large-scale production of P. peruviana (L.) due to the elimination of sexual propagation.

PL

Gatunek Physalis peruviana L. należy do rodziny Solanaceae i znany jest powszechnie jako miechunka peruwiańska. Ostatnio jest bardzo popularny i używany jako roślina lecznicza w terapii malarii, astmy, zapalenia wątroby, zaplenia skóry i reumatyzmu. Posiada właściwości moczopędne i przeciwzapalne. Celem badania było opracowanie protokołu rozmnażania in vitro dla Physalis peruviana L. przy użyciu szczytowych pędów jako źródła eksplantów. Zregenerowane rośliny oceniono na podstawie ich współczynnika rozmnażania oraz długości pędów przy użyciu różnych stężeń BAP (1, 2, 3 mg l-1) w kombinacji z IBA (0, 0,1, 0,2, 0,4 mg l-1) oraz NAA (0, 0,1, 0,2, 0,4 mg l-1). Ponadto sprawdzono też skuteczność różnych koncentracji auksyn (1 i 2 mg l- 1 IBA i NAA) w tworzeniu korzeni P. peruviana L. Największą liczbę pędów otrzymano z kombinacji 2 mg l- 1 BAP z 0.4 mg l- 1 IBA (6.00) i dla długości pędów w kombinacji 2 mg l- 1 BAP z 0.2 mg l- 1 IBA (3,30 cm). Odnośnie do efektów kombinacji BAP i NAA: największą długość pędów otrzymano z kombinacji 2 mg l- 1 BAP bez NAA (3,33 cm) natomiast najmniejszą z kombinacji 3 mg l- 1 BAP z 0,4 mg/I NAA. Największą liczbę korzeni otrzymano przy zastosowaniu NAA (odpowiednio, 2 mg l- 1 i 1 mg l-1). Rośliny uzyskane in vitro dobrze aklimatyzowały się w warunkach glebowych. Badanie podkreśla znaczenie hodowli tkanek roślin w celu wykorzystania ich do produkcji P. peruviana (L.) na dużą skalę ze względu na eliminację rozmnażania płciowego.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2016
• Tom: 15
• Numer: 5
• Opis fizyczny: p.109-118,fig.,ref.

Twórcy

autor

Guney M.

• Cukurova University of Cukurova, Balcalı, Adana, Turkey

autor

Kafkas S.

• Cukurova University of Cukurova, Balcalı, Adana, Turkey

autor

Kefayati S.

• Cukurova University of Cukurova, Balcalı, Adana, Turkey

autor

Motalebipour Z.

• Cukurova University of Cukurova, Balcalı, Adana, Turkey

autor

Turkeli Y.

• Ministry of Food Agriculture and Livestock, Ankara, Turkey

autor

Ercisli S.

• Department of Horticulture, Faculty of Agriculture, Ataturk University, 25240 Erzurum, Turkey

autor

Kafkas E.

• Cukurova University of Cukurova, Balcalı, Adana, Turkey

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