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2015 | 59 | 1 |

Tytuł artykułu

Genetic typing of bovine viral diarrhoea virus (BVDV) by restriction fragment length polymorphism (RFLP) and identification of a new subtype in Poland

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Restriction fragment length polymorphism (RFLP) analysis was developed for genetic ty ping of Polish strains of bovine viral diarrhoea virus (BVDV). The method was applied using 60 BVDV isolates, which included BVDV genotype 1, subtypes a, b, d, e, f, and g, and genotype 2a. RT-PCR products of the 5'untranslated region (5'UTR) were digested using three enzymes. Restriction patterns classified the strains into seven groups, each with a specific and different pattern from other subty pes. These findings were confirmed by nucleotide sequencing and phylogenetic analysis. The results suggest that RFLP analysis is a simple, reliable, and fast genotyping method for BVDV strains in comparison with sequencing. This method can distinguish six subtypes of BVDV-1 including a new subtype 1e, identified exclusively by this method, and it allows differentiation of BVDV-1 from BVDV-2 genotype.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

59

Numer

1

Opis fizyczny

p.19-22,fig.,ref.

Twórcy

autor
  • Department of Virology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Virology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Virology, National Veterinary Research Institute, 24-100 Pulawy, Poland
  • Department of Virology, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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