Bacterial communities in Branchinella thailandensis and Streptocephalus sirindhornae

• Autorzy: Peerakietkhajorn S. , Sinso T.
• Języki publikacji: EN

Abstrakty

EN

The fairy shrimp is a freshwater crustacean found in both temporary and permanent freshwaters. In Thailand, fairy shrimp are farmed as live food for ornamental fish. This study aimed to investigate the bacterial compositions in two fairy shrimp species, Branchinella thailandensis and Streptocephalus sirindhornae. Both species were cultured, and total DNA was extracted. The V3-V4 region of the 16S rRNA gene was amplified and sequenced using Illumina Miseq. All data were analyzed by Illumina 16S Metagenomics (version 1.0.1) workflow in Base SpaceIllumina. Each read was blasted against the Illumina-curated version of the Greengenes database to determine the operational taxonomic units (OTUs) corresponding to the 16S rRNA gene sequence. The results showed that the Shanon-Weiner diversity index of bacterial compositions in B. thailandensis and S. sirindhornae were 2.135 and 3.122, respectively. The evenness and genus-level richness of the bacterial composition in B. thailandensis were 0.364 and 354 genera, respectively. The dominant bacterium found in B. thailandensis was Nevskia. In S. sirindhornae, the evenness and genus-level richness of the bacterial composition were 0.521 and 400 genera, respectively. Azohydromonas was the dominant bacterium. Our results showed that the compositions and proportions of bacterial communities were specific to each species of fairy shrimp. This study will be useful for further experiments in aquaculture and ecological studies related to symbiotic interaction.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2022
• Tom: 25
• Numer: 4
• Opis fizyczny: p.493-500,fig.,ref.

Twórcy

autor

Peerakietkhajorn S.

• Division of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand
• Gut Biology and Microbiota Research Unit, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand

autor

Sinso T.

• Division of Biological Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand

Identyfikatory

DOI

10.24425/pjvs.2022.140857