Nitric oxide synthesis inhibitor increases severity of homocysteine-induced seizures in adult rats

• Autorzy: Hrncic D. , Rasic-Markovic A. , Susik V. , Djuric D. , Stanojlovic O.
• Języki publikacji: EN

Abstrakty

EN

Homocysteine, endogenous sulphur – containing amino acid, displays neuroexcitatory effects and acts as a convulsant agent by still unclear mechanisms. Reported results on the role of nitric oxide (NO) in epileptogenesis are highly contradictory. NO levels could be decreased by N-nitro-L-arginine methyl ester (L-NAME), an inhibitor of enzyme NO synthase (NOS). The aim of the current study was to examine the effects of L-NAME on seizure susceptibility induced by subconvulsive dose of D,L homocysteine – thiolactone (HCT) in rats. Adult male Wistar albino rats were intraperitoneally (i.p.) treated with HCT 5.5 mmol/kg and observed for seizure behavioral manifestations during next 90 min. Increasing doses of L-NAME (200, 500 and 700 mg/kg, i.p., n=6, 7 and 8, respectively) or saline (n=9) were injected 30 min prior to HCT administration. Seizure episode severity was assessed using descriptive rating scale with four grades. L-NAME in doses of 500 and 700 mg/kg signifi cantly increased severity of homocysteine seizures (P<0.05) when compared with saline –injected rats. The lowest applied dose of L-NAME (200 mg/kg) had no statistically signifi cant effect on severity of seizures induced by HCT. Based on these results it cold be concluded that L-NAME, NOS inhibitor, potentiates convulsive properties of HCT.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.319-320

Twórcy

autor

Hrncic D.

• Laboratory of Neurophysiology, Institute of Medical Physiology Richard Burian, Belgrade University School of Medicine, Belgrade, Serbia

autor

Rasic-Markovic A.

• Laboratory of Neurophysiology, Institute of Medical Physiology Richard Burian, Belgrade University School of Medicine, Belgrade, Serbia

autor

Susik V.

• Department of Medical Sciences SASA, Belgrade, Serbia

autor

Djuric D.

• Laboratory of Neurophysiology, Institute of Medical Physiology Richard Burian, Belgrade University School of Medicine, Belgrade, Serbia

autor

Stanojlovic O.

• Laboratory of Neurophysiology, Institute of Medical Physiology Richard Burian, Belgrade University School of Medicine, Belgrade, Serbia