Determination of T-2 and HT-2 toxins in feedstuffs by high-performance liquid chromatography with fluorescence detector

• Autorzy: Burek O. , Wisniewska-Dmytrow H. , Zmudzki J.
• Języki publikacji: EN

Abstrakty

EN

The procedure for the determination of T-2 and HT-2 toxins in feedstuffs has been developed. The test portion was extracted with methanol-water (80:20, v/v) after adding sodium chloride and cleaned-up through immunoaffinity column. Mycotoxins were quantified by a high-performance liquid chromatography coupled with fluorescence detector (λex=381 nm, λem=470 nm) after pre-column derivatisation, which was achieved with 4-dimethylaminopyridine and 1-anthroyl cyanide solutions. The method was validated according to the Commission Regulation (EC) No 401/2006. Average recoveries for the compounds ranged from 94.0% to 106.5% for T-2 toxin and from 80.3% to 102.9% for HT-2 toxin. The limits of detection and quantification for T-2 and HT-2 toxins were 6 and 15 µg kg⁻¹, respectively.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2011
• Tom: 55
• Numer: 4
• Opis fizyczny: p.737-740,fig.,ref.

Twórcy

autor

Burek O.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Wisniewska-Dmytrow H.

autor

Zmudzki J.

Bibliografia

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