Antioxidant responses and water status in Brassica seedlings subjected to boron stress

• Autorzy: Pandey N , Archana
• Języki publikacji: EN

Abstrakty

EN

The effect of boron (B) on growth, water status and oxidative damage was investigated in the leaves and roots of 7-day-old seedlings of Brassica juncea var.Varuna.For this seedlings of Brassica were grown in solution culture with variable boron supply (0.033, 0.33, 3.3 and 33 mg B L-1) under controlled conditions in green house. Photosynthetic pigments were found to be decreased more under excess (3.3 and 33 mg B L–1) than deficient boron supply (0.033 mg B L–1) when compared to control (0.33 mg B L–1). Accumulation of hydrogen peroxide and thiobarbituric acid reactive substances content in both leaves and roots under deficient and excess boron supply suggested oxidative damage due to excessive production of reactive oxygen species. Increased activity of antioxidative enzymes: superoxide dismutase, catalase and peroxidase along with polyphenol oxidase was observed in leaves and roots under boron deficiency and excess than in control. Increased proline concentration, decreased total water content and water saturation deficit also indicated the water deficit condition in leaves and roots of boron-stressed Brassica seedlings.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 03
• Opis fizyczny: p.697-706,fig.,ref.

Twórcy

autor

Pandey N

• Plant Nutrition and Stress Physiology Laboratory, Department of Botany, University of Lucknow, Lucknow 226007, India

autor

Archana

• Plant Nutrition and Stress Physiology Laboratory, Department of Botany, University of Lucknow, Lucknow 226007, India

Bibliografia

• Ahmed M, Jahiruddin M, Mian MH (2007) Screening of wheat genotype for boron efficiency. J Plant Nutr 30:1127–1138
• Apostol KG, Zwiazek JJ (2004) Boron and water uptake in jack pine (Pinus banksiana) seedlings. Environ Exp Bot 51:145–153
• Apostol KG, Zwiazek JJ, MacKinnon MD (2002) NaCl and Na2SO4 alter responses of jack pine (Pinus banksiana) seedlings to boron. Plant Soil 240:321–329
• Ardıc M, Sekmen AH, MacKinnon MD, Turkan I, Tokur S, Ozdemir F (2009) The effects of boron toxicity on root antioxidant systems of two chickpea (Cicer arietinum L.) cultivars. Plant Soil 314:99–108
• Baisak R, Rana D, Acharaya PB, Kar M (1994) Alterations in the activities of active oxygen scavenging enzymes of wheat leaves subjected to water stress. Plant Cell Physiol 35:489–495
• Bastı´as E, Ferna´ndez-Garcı´a N, Carvajal M (2004) Aquaporin functionality in roots of Zea mays in relation to the interactive effects of boron and salinity. Plant Biol 5:415–421
• Bastı´as E, Alcaraz-Lo´pez C, Bonilla I, Martı´nez-Ballesta MC, Bolano˜s L, Carvajal M (2010) Interactions between salinity and boron toxicity in tomato plants involve apoplastic calcium. J Plant Physiol 167:54–60
• Bates LS, Walderen RD, Taere ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207
• Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gel. Anal Biochem 44:276–287
• Becker DF, Zhu W, Natarajan S (2009) Proline metabolism and protection against oxidative stress. FASEB J 23(678):7
• Bellaloui N, Brown PH (1998) Cultivar differences in boron uptake and distribution in celery (Apium graveolens), tomato (Lycopersicon esculentum) andwheat (Triticumaestivum). Plant Soil 198:153–158
• Blevins DG, Lukaszewski KM (1998) Boron in plant structure and function. Ann Rev Plant Physiol Plant Mol Biol 49:481–501
• Brennan T, Frenkel C (1977) Involvement of hydrogen peroxide in the regulation of senescence in pear. Plant Physiol 59:411–416
• Brown PH, Bellaloui N, Wimmer MA, Bassil ES, Ruiz J, Hu H, Pfeffer H, Dannel F, Ro¨mheld V (2002) Boron in plant biology. Plant Biol 4:205–223
• Cakmak I, Ro¨mheld V (1997) Boron deficiency induced impairment of cellular functions in plant. Plant Soil 193:71–83
• Cakmak I, Kurz H, Marschner H (1995) Short-term effects of boron, germanium and high light intensity on membrane permeability in boron deficient leaves of sunflower. Physiol Plant 95:11–18
• Camacho-Cristo´bal JJ, Herrera-Rodrı´guez MB, Beato VM, Rexach J, Navarro-Gochicoa MT, Maldonado JM, Gonza´lez-Fontes A (2008) The expression of several cell wall-related genes in Arabidopsis roots is down-regulated under boron deficiency. Environ Exp Bot 63:351–358
• Cervilla LM, Blasco B, Rios JJ, Romero L, Ruiz JM (2007) Oxidative stress and antioxidants in tomato (Solanum lycopersicum) plants subjected to boron toxicity. Ann Bot 100:747–756
• Choi EY, Kolesik P, Mcneill A, Collins H, Zhang Q, Huynh B et al (2007) The mechanism of boron tolerance for maintenance of root growth in barley (Hordeum vulgare L.). Plant Cell Environ 30:984–993
• Clarkson DT, Carvajal M, Henzler T, Waterhouse RN, Smyth AJ, Cooke DT, Steudle E (2000) Root hydraulic conductance: diurnal aquaporin expression and the effects of nutrient stress. J Exp Bot 51:61–70
• Dannel F, Pfeffer H, Ro¨mheld V (1998) Compartmentation of boron in roots and leaves of sunflower as affected by boron supply. J Plant Physiol 153:615–622
• Demmig-Adams B, Adams WW III (1996) Xanthophyll cycle and light stress in nature: uniform response to excess direct sunlight among higher plant species. Planta 198:460–470
• Eraslan F, Inal A, Gunes A, Alpaslan M (2007) Boron toxicity alters nitrate reductase activity, proline accumulation, membrane permeability and mineral constituents of tomato and pepper plants. J Plant Nutr 30:981–994
• Han S, Tang N, Jiang H, Yang L, Yan L, Chen L (2009) CO2 assimilation photosystem II photochemistry, carbohydrate metabolism and antioxidant system of citrus leaves in response to boron stress. Plant Sci 176:143–153
• Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
• Hoagland DR, Arnon DI (1950) The water culture method for growing plants without soil. Calif Agric Exp Stn 347(2):32
• Josten P, Kutschera U (1999) The micronutrient boron causes the development of adventitious roots in sunflower cuttings. Ann Bot 84:337–342
• Karabal E, Yucel M, Oktem H (2003) Antioxidant responses of tolerant and sensitive barley cultivars to boron toxicity. Plant Sci 164:925–933
• Kobayashi M, Matoh T (2004) Boron nutrition of cultured tobacco BX-2 cells. IV. Genes induced under low B supply. J Exp Bot 55:1441–1443
• Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148:350–382
• Liu P, Yang YA (2000) Effects of molybdenum and boron on membrane lipid peroxidation and endogenous protective systems of soybean leaves. Acta Bot Sin 42:461–466
• Loomis WD, Durst RW (1992) Chemistry and biology of boron. BioFactors 3:229–239
• Mannan MA, Karim MA, Khaliq QA, Haque MM, Mian MAK, Ahmed JU (2009) Proline accumulation, water status and chlorophyll content in leaf in relation to salt tolerance in soybean. Ind J Plant Physiol 14:130–134
• Martı´nez-Ballesta MC, Bastı´as E, Zhu C, Scha¨ffner A, Gozalez-Murua B, Gonzalez-Moro C, Carvajal M (2008) Boric acid and salinity effects on maize roots. Response of aquaporins ZmPIP1 and ZmPIP2 and plasma membrane H?-ATPase in relation to water and nutrient uptake. Physiol Plant 132:479–490
• Nable RO, Banuelos GS, Paull JG (1997) Boron toxicity. Plant Soil 198:181–198
• Ohtuska T, Ito H, Tanaka A (1997) Conversion of chlorophyll b to chlorophyll a and the assembly of chlorophyll with apoprotein by isolated chloroplast. Plant Physiol 113:137–147
• Pandey N, Archana (2009) Boron-stress induced changes in water status and stomatal morphology in Zea mays L. and Catharanthus roseus L. Ind J Plant Physiol 14:310–314
• Pandey N, Archana (2010) Effect of boron on mitotic index and seed germination of sunflower. J Ind Bot Soc 89:293–296
• Pandey N, Sharma CP (2002) Effect of heavy metals Co2+, Ni2+ and Cd2+ on growth andmetabolismof cabbage. Plant Sci 163:753–758
• Papadakis I, Dimassi K, Bosabalidis A, Therios I, Patakas A, Ginnakoula A (2004) Boron toxicity in ‘climentine’ mandarin plants grafted on two root stocks. Plant Sci 166:539–547
• Plesnica´r M, Kastori R, Sakac Z, Pankovic D, Petrovic N (1997) Boron as limiting factor in photosynthesis and growth of sunflower plants in relation to phosphate supply. Agrochimica 41:144–154
• Polovnikova MG, Voskresenskaya OL (2008) Activities of antioxidant system components and polyphenol oxidase in ontogeny of lawn grasses under megapolis conditions. Russ J Plant Physiol 55:699–705
• Power PP, Woods WG (1997) The chemistry of boron and its speciation in plants. Plant Soil 193:1–13
• Sairam RK, Deshmukh PS, Saxsena DC (1998) Role of antioxidant system in wheat genotypes tolerance to water stress. Biol Plant 41:384–394
• Samaras Y, Bressan RA, Csonka LN, Garcia-Rios MG, Paino D, Urzo M, Rhodes D (1995) Proline accumulation during drought and salinity. In: Smirnoff N (ed) Environment and plant metabolism. Bio Scientific Publishers, Oxford, pp 161–187
• Sangakkara UR, Hartwig UA, Nosberger J (1996) Responses of root branching and shoot water potential of French bean (Phaseolus vulgaris L) to soil moisture and fertilizer potassium. J Agron Crop Sci 177:165–173
• Sharma PN, Ramachandra T (1990) Water relations and photosynthesis in mustard plants subjected to boron deficiency. Ind J Plant Physiol 33:150–154
• Sharma CP, Sharma PN (1987) Mineral nutrient deficiencies affect plant water relations. J Plant Nutr 10:1637–1643
• Shelp BJ (1988) Boron mobility and nutrition in broccoli (Brassica oleracea var. Italica). Ann Bot 61:83–91
• Shelp BJ (1993) Physiology and biochemistry of boron in plants. In: Gupta UC (ed) Boron and its role in crop production. CRC Press, Boca Raton, pp 53–85
• Shenshi H, Noguchi M (1975) Relationship between peroxidase, IAA oxidase and polyphenoloxidase. Phytochemistry 14:1255–1258
• Sieferman-Harms D (1987) The light harvesting and protective function of carotenoids in photosynthetic membrane. Physiol Plant 69:561–568
• Takano J, Noguchi K, Yasumori M, Kobayashi M, Gajdos Z, Miwa K, Hayashi H, Yoneyama T, Fujiwara T (2002) Arabidopsis boron transporter for xylem loading. Nature 420:337–340
• Takano J, Wada M, Ludewig U, Schaaf G, von Wire´n N, Fujiwara T (2006) The Arabidopsis major intrinsic protein NIP5;1 is essential for efficient boron uptake and plant development under boron limitation. Plant Cell 18:1498–1509
• Wan X, Zwiazek JJ (2001) Root water flow and leaf stomatal conductance in aspen (Populus tremuloides) seedlings treated with abscisic acid. Planta 213:741–747
• Wang ZY, Tang YL, Zhang FS, Wang H (1999) Effect of boron and low temperature on membrane integrity of cucumber leaves. J Plant Nutr 22:543–550
• Wolf B (1971) The determination of boron in soil extracts, plant materials, composts, water and nutrient solutions. Commun Soil Sci Plant Anal 2:363–374

Uwagi

rekord w opracowaniu