Influence of isopropyl methylphosphonofluoridate intoxication on cytokine levels in chosen brain structures

• Autorzy: Kowalczyk M. , Zdanowski R. , Antkowiak B. , Paluch M.
• Języki publikacji: EN

Abstrakty

EN

Poisoning with organophosphate compounds induces pronounced neurotransmitter disturbances in central nervous system. There are some data indicating that these poisonings initiate changes in neuro-immune response. Our study was aimed at the evaluation of cytokines levels (interleukin 1β – IL-1β, interleukin 2 – IL-2, tumor necrosis factor α – TNF-α and interferon γ – IFNγ) after isopropyl methylphosphonofl uoridate (IMPF) intoxication in chosen brain structures of BALB/c mice. Tests were performed in supernatants of frontal cortex, hippocampus and brain stem homogenates using immunoenzymatic assay after 24 h, 7, 14 and 21 days of IMPF intoxication (1/2 LD50, s.c.). IMPF induced differential changes in cytokines levels (except IL-2) in the examined brain structures. The signifi cant increase of TNF-α and IL-1β levels was shown in frontal cortex after 14 days of poisoning. Raised level of TNF-α in this structure was observed until the end of the experiment (21st day). We also observed pronounced increase of IFNg level after 7 days in hippocampus and after 7 and 14 days in brain stem. Concurrently the level of IL-1β in these structures was signifi cantly decreased, in hippocampus in all measurements, while in the brain stem only after 24 hours following IMPF application.Our results indicate that IMPF intoxication without inducing convulsions can produce marked changes in brain cytokines levels that may lead to immune response disturbances.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.318

Twórcy

autor

Kowalczyk M.

• Department of Pharmacology and Toxicology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland

autor

Zdanowski R.

• Department of Pharmacology and Toxicology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland

autor

Antkowiak B.

• Department of Pharmacology and Toxicology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland

autor

Paluch M.

• Department of Pharmacology and Toxicology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland