Cellulose-degrading strains: their screening and application to corn straw in low-temperature environments

• Autorzy: Zhang S. , Shan D. , Liu X. , Sun M.
• Języki publikacji: EN

Abstrakty

EN

Cellulose-degrading strains play an important role in cellulose degradation at low temperatures. In this study, three cellulose-degrading strains with high carboxymethyl cellulase (CMCase) activity were isolated from the soil in a low temperature environment of 10ºC. ZS-7 was Cladosporium sp, ZS-8 was Alternaria porri, and ZS-10 was Trichoderma harzianum, based on 16s rDNA gene sequence analysis. They were mixed, marked as ZS-M, and used for corn straw degradation at 15ºC in order to determine their application properties. ZS-7, ZS-8, ZS-10, and ZS-M efficiently degraded corn straw by 45.98%, 47.96%, 34.215%, and 33.56%, respectively, after 30 days of stationary culture. The dynamics of CMC, β-Glucosidase, and FPA during corn straw degradation were determined. The CMCase activity of ZS-7 and ZS-8 was 31.732 U/ml and 31.111 U/ml, respectively, but the enzyme activities and degradation of ZS-M were lower than the single strain because of metabolic effects that influenced the degradation process. The pH values of ZS-7, ZS-10, and ZS-M were particularly stable, changing from 7.08 to 7.77 during the full degradation cycle. ZS-7, ZS-8, and ZS-10 could be applied more broadly to degrade corn straw in cold regions.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2018
• Tom: 27
• Numer: 5
• Opis fizyczny: p.2349-2355,fig.,ref.

Twórcy

autor

Zhang S.

• College of Resource and Environment, Northeast Agricultural University, Harbin, China

autor

Shan D.

• College of Resource and Environment, Northeast Agricultural University, Harbin, China

autor

Liu X.

• College of Resource and Environment, Northeast Agricultural University, Harbin, China

autor

Sun M.

• College of Resource and Environment, Northeast Agricultural University, Harbin, China

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Identyfikatory

DOI

10.15244/pjoes/79272