Loss of protein expression and recurrent DNA hypermethylation of the GNG7 gene in squamous cell carcinoma of the head and neck

• Autorzy: Hartmann S. , Szaumkessel M. , Salaverria I. , Simon R. , Sauter G. , Kiwerska K. , Gawecki W. , Bodnar M. , Marszalek A. , Richter J. , Brauze D. , Zemke N. , Jarmuz M. , Hansmann M.-L. , Siebert R. , Szyfter K. , Giefing M.
• Języki publikacji: EN

Abstrakty

EN

Although down-regulation of GNG7 in cancer was reported before, its role in carcinogenesis is poorly understood. It belongs to a family of large G-proteins that may be involved in cell-contact-induced growth arrest and function in tumor suppression. In the present study, we stained immunohistochemically 188 tumors derived from larynx or floor of the mouth for GNG7 protein and confronted it with clinicopathologic data. Moreover, we performed bisulfite pyrosequencing to analyze GNG7 promoter methylation. We identified recurrent loss of GNG7 protein expression in 68/188 (36%) cases and promoter hypermethylation in (42/98; 43%) primary tumors, predominantly in young patients (p<0.001). Loss of GNG7 expression correlated with hypermethylation of GNG7 promoter region (p<0.001). Moreover, loss of GNG7 protein expression correlated with tumor size (p=0.012) and lack of cervical metastasis (p=0.02) whereas sustained expression correlated with keratinization (p=0.008). Taken together, loss of GNG7 protein expression is a frequent event in head and neck cancer. Moreover, our data suggest that hypermethylation of the promoter region of GNG7 is probably the mechanism of the observed inactivation.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2012
• Tom: 53
• Numer: 2
• Opis fizyczny: p.167-174,fig.,ref.

Twórcy

autor

Hartmann S.

• Institute of Pathology, University Hospital, 60590 Frankfurt, Germany

autor

Szaumkessel M.

• Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland

autor

Salaverria I.

autor

Simon R.

autor

Sauter G.

autor

Kiwerska K.

• Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland

autor

Gawecki W.

• Department of Otolaryngology, University of Medical Sciences, 60-355 Poznan, Poland

autor

Bodnar M.

• Department of Clinical Pathomorphology, Collegium Medicum, 85-094 Bydgoszcz, Poland

autor

Marszalek A.

• Department of Clinical Pathomorphology, Collegium Medicum, 85-094 Bydgoszcz, Poland

autor

Richter J.

autor

Brauze D.

• Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland

autor

Zemke N.

• Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland

autor

Jarmuz M.

• Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland

autor

Hansmann M.-L.

autor

Siebert R.

autor

Szyfter K.

• Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland

autor

Giefing M.

• Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland

Uwagi

PL

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