Real-time VSD based optical imaging revealed the biodirectional interactions of ongoing activity and sensory-evoked activity

• Autorzy: Grinvald A.
• Języki publikacji: EN

Abstrakty

EN

To better understand cortical processing, principles underlying the neural code and higher brain functions, many technical requirements must be met by tools used to measure brain electrical activity, all the way from the single cell level to the entire brain. Remarkable progress has been accomplished with the development of genetically engineered calcium and voltage-sensitive probes, and two photon imaging technologies. However, all available tools are currently falling short in meeting one or more of the technological requirements. Here we describe an advance in optical imaging of spatio-temporal pattern of coherent population neuronal activity, millisecond by millisecond, in-vivo. We integrated this technique with other neurophysiological techniques in order to explore the role of ongoing activity on brain function and behavior. We designed and synthetized a superior organic voltage sensitive dye (VSDs) offering remarkable signal-to-noise ratio in a single trial with sub-millisecond time resolution and detection both subthreshold and spiking coherent activity, distinctly. Exploring the dynamics of ongoing cortical activity we discovered that ongoing activity accounts for the large variability of the evoked activity. In exploring anesthetized and behaving non-human primates we found similarity and differences. We also explored the interaction in the opposite direction and found that sensory evoked activity diminished ongoing activity. Imaging tools to detect both the input to and output produced by coherently active neuronal populations, ms by ms, over a large cortical area are available for revolutionizing our current understanding of neuronal processing. Intrinsic cortical activity and extrinsic sensory-evoked activity interacts. These activities are composed of coherent spatio-temporal patterns of synaptic potentials and Coherent Population Spikes rather than by the same individual cells.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S12

Twórcy

autor

Grinvald A.

• Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel