Chemical composition and antifungal activities of Ziziphora tenuior and Z. clinopodioides essential oils against dermatophytes

• Autorzy: Mahboubi M. , Tabar R.H. , Mahdizadeh E.

Warianty tytułu

PL

Skład chemiczny i działanie przeciwgrzybicze olejków eterycznych z Ziziphora tenuior i Z. clinopodioides przeciw dermatofitom

• Języki publikacji: EN

Abstrakty

EN

Ziziphora species are traditionally used for treatment of different infectious and non-infectious diseases as antiseptic agents. The aim of this study was to evaluate the chemical composition of Ziziphora clinopodioides and Z. tenuir essential oils and their antifungal effects againt five strains of dermatophytes. GC and GC-MS methods were used for essentional oils analize. The anti-elastase activities were determined by porcine pancreatic elastase assays. 48 different compounds were identified in these two essential oils, which thymol, p-cymene, 1,8-cineole and γ-terpinene were their major components. The anti-dermatophyte activities of essential oils against dermatophytes showed that the essential oils (150 ppm) inhibited the mycelium growth, about 5–100%, which Z. clinopodioides essential oil had higher mycelium growth inhibition (28–100%) than that of Z. tenuir oil. The MIC and MFC values of essential oils were 0.01–1 μl/ml. 0.5 μl/ml essential oils inhibited porcine pancreatic elastase, dose-dependently. Due to the anti-dermatophyte and anti-elastase effects of Ziziphora sp., it can be considered as natural antifungal agent for more clinical and pre-clinical trials.

PL

Rośliny z rodzaju Ziziphora są tradycyjnie stosowane w leczeniu różnych chorób infekcyjnych i nieinfekcyjnych ze względu na swe działanie antyseptyczne. Celem pracy było określenie składu chemicznego olejków eterycznych Ziziphora clinopodioides i Z. tenuir oraz działania przeciwgrzybiczego przeciwko pięciu dermatofitom. Skład chemiczny olejków badano za pomocą GC i GC-MS. Działanie antyelastazowe badano z wykorzystaniem świńskiej trzustkowej elastazy. W dwóch olejkach zidentyfikowano 48 różnych składników, wśród których głównymi były tymol, p-cymen, 1,8-cyneol i γ-terpinen. Badanie aktywności antydermatofitowej olejków pokazało, że olejki eteryczne (150 ppm) hamowały wzrost mycelium o około 5–100%. Olejek eteryczny z Z. clinopodioides bardziej hamował wzrost mycelium (28–100%) niż olejek z Z. tenuir. Wartości MIC i MFC olejków eterycznych wynosiły 0,01–1 μl/ml. Stężenie 0,5 μl/ml olejków eterycznych hamowało elastazę trzustki świńskiej w sposób uzależniony od dawki. Z powodu działania antydermatofitowego i antyelastazowego Ziziphora może być uważana za naturalny środek przeciwgrzybiczy, który wymaga dalszych badań przedklinicznych i klinicznych.

Słowa kluczowe

EN

Ziziphora tenuior; Ziziphora clinopodioides; essential oil; chemical composition; antifungal activity; dermatophyte

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2018
• Tom: 64
• Numer: 2
• Opis fizyczny: p.37-45,fig.,ref.

Twórcy

autor

Mahboubi M.

• Department of Microbiology, Medicinal Plants, Research Center of Barij, Kashan, Iran

autor

Tabar R.H.

• Department of Microbiology, Medicinal Plants, Research Center of Barij, Kashan, Iran

autor

Mahdizadeh E.

• Department of Microbiology, Medicinal Plants, Research Center of Barij, Kashan, Iran

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Identyfikatory

DOI

10.2478/hepo-2018-0011