High occurrence of Cryptosporidium ubiquitum and Giardia duodenalis genotype E in sheep from Brazil

• Autorzy: Silva F.M.P. , Lopes R. S. , Bresciani K. D. S. , Amarante A. F. T. , Araujo Jr J. P.
• Języki publikacji: EN

Abstrakty

EN

In this paper we report the occurrence of zoonotic species of Cryptosporidium (C. ubiquitum and C. parvum subtype IIaA15G2R1) and the host-specific Giardia duodenalis genotype E in a sheep farm from Brazil. Fecal specimens were collected from 100 animals and screened for the presence of G. duodenalis and Cryptosporidium spp. by enzyme immunoassay (EIA) and polymerase chain reaction (PCR). Restriction fragment length polymorphism (RFLP) and sequence analysis of selected samples was used to confirm the species and to characterize the isolates. Of 100 specimens, 34 were positive for Giardia (34%) and 25 were positive for Cryptosporidium (25%). The prevalence of Giardia and Cryptosporidium was higher in lambs than in ewes. Co-infection was observed in 14% of animals. All G. duodenalis isolates belonged to genotype E. The genetic characterization performed in 22 Cryptosporidium-positive samples revealed the presence of zoonotic C. ubiquitum and C. parvum in 76% (19/25) and 12% (3/25) of infected animals, respectively. The presence of zoonotic Cryptosporidium subtypes in lambs was of epidemiological and public health relevance.

Słowa kluczowe

EN

Cryptosporidium ubiquitum; Giardia duodenalis; genotype; sheep; Brazil; zoonosis; enzyme immunoassay; polymerase chain reaction; restriction fragment length polymorphism

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2014
• Tom: 59
• Numer: 1
• Opis fizyczny: p.193-196,ref.

Twórcy

autor

Silva F.M.P.

• Laboratory of Parasitic Diseases, Department of Veterinary Clinic, Faculty of Veterinary Medicine and Animal Science, UNESP – Univ Estadual Paulista, Botucatu, Sao Paulo, Brazil

autor

Lopes R. S.

• Laboratory of Parasitic Diseases, Department of Veterinary Clinic, Faculty of Veterinary Medicine and Animal Science, UNESP – Univ Estadual Paulista, Botucatu, Sao Paulo, Brazil

autor

Bresciani K. D. S.

• Departamento de Apoio, Producao e Saude Animal, FMVA, UNESP – Univ Estadual Paulista, Aracatuba, Sao Paulo, Brazil

autor

Amarante A. F. T.

• Department of Parasitology, Biosciences Institute, UNESP – Univ Estadual Paulista, Botucatu, Sao Paulo, Brazil

autor

Araujo Jr J. P.

• Laboratory of Molecular Diagnosis, Department of Microbiology and Immunology, Biosciences Institute, UNESP - Univ Estadual Paulista, Botucatu, Sao Paulo, Brazil

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