Molecular characterization and expression patterns of sucrose transport-related genes in sweet sorghum under defoliation

• Autorzy: Li X. , Su M. , Li X. , Cheng L. , Qi D. , Chen S. , Liu G.
• Języki publikacji: EN

Abstrakty

EN

Sucrose is the principal form of photosynthesis products, and long-distance transport of sucrose requires sucrose transporters (SUTs) to perform loading and unloading functions. SUTs play an important role in plant growth, development and reproduction. In this study, five unique sucrose transporter (SbSUT) genes that contain full-length cDNA sequences were cloned from sweet sorghum, and these SbSUT genes were clustered into four different clades: SUT1, SUT3, SUT4 and SUT5. Heterologous expression of SbSUTs in yeast demonstrated that they were functional sucrose transporters. Tissue-specific expression profiles showed that sorghum SUT genes had different tissue-specific expression patterns, suggesting that sorghum SUT genes may play an important role in plant growth and developmental processes. After defoliation, expression patterns of SbSUT1, SbSUT2 and SbSUT4 were different in leaf sheaths, leaves and roots. Taken together, the results indicate that the above mentioned five unique sucrose transporter genes may play important roles in performing sucrose loading and unloading functions and that they exhibit different expression in response to leaf blade removal.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 05
• Opis fizyczny: p.1251-1259,fig.,ref.

Twórcy

autor

Li X.

• Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People's Republic of China

autor

Su M.

• Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People's Republic of China

autor

Li X.

• Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People's Republic of China

autor

Cheng L.

• Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People's Republic of China

autor

Qi D.

• Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People's Republic of China

autor

Chen S.

• Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People's Republic of China

autor

Liu G.

• Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People's Republic of China

Bibliografia

• Aoki N, Hirose T, Scofield GN, Whitfeld PR, Furbank RT (2003) The sucrose transporter gene family in rice. Plant Cell Physiol 44:223–232
• Barker L, Kühn C, Weise A, Schulz A, Gebhardt C, Hirner B, Hellmanna H, Schulze W, Ward JM, Frommer WB (2000) SUT2, a putative sucrose sensor in sieve elements. Plant Cell 12:1153–1164
• Berthier A, Desclos M, Amiard V, Morvan-Bertrand A, Demmig-Adams B, William WA, Turgeon R, Prud’homme M, Noiraud-Romy N (2009) Activation of sucrose transport in defoliated Lolium perenne L.: an example of apoplastic phloem loading plasticity. Plant Cell Physiol 50:1329–1344
• Braun DM, Slewinski TL (2009) Genetic control of carbon partitioning in grasses: roles of sucrose transporters and Tie-dyed loci in phloem loading. Plant Physiol 149:71–81
• Callow MN, Michell P, Baker JE, Cocks PS, Houg GM (2005) Response of herbage regrowth and water-soluble carbohydrate concentration of ryegrass species to defoliation practices when grown in a Mediterranean environment. Grass Forage Sci 60:59–67
• Carpaneto A, Geiger D, Bamberg E, Sauer N, Fromm J, Hedrich R (2005) Phloem-localized, proton-coupled sucrose carrier ZmSUT1 mediates sucrose efflux under the control of the sucrose gradient and the proton motive force. J Biol Chem 280:21437–21443
• Chandran D, Reinders A, Ward JM (2003) Substrate specificity of the Arabidopsis thaliana sucrose transporter AtSUC2. J Biol Chem 278:44320–44325
• Chen JY, Liu SL, Siao W, Wang SJ (2010) Hormone and sugar effects on rice sucrose transporter OsSUT1 expression in germinating embryos. Acta Physiol Plant 32:749–756
• Daie J (1989) Phloem loading of sucrose: update and opportunities in molecular biology. Plant Mol Biol Rep 7:106–115
• Doidy J, Tuinen DV, Lamotte O, Corneillat M, Alcaraz G, Wipf D (2012) The Medicago truncatula sucrose transporter family: characterization and implication of key member in carbon partitioning towards arbuscular mycorrhizal fungi. Mol Plant 5:1346–1358
• Hirose T, Imaizumi N, Scofield GN, Furbank RT, Ohsugi R (1997) cDNA cloning and tissue specific expression of a gene for sucrose transporter from rice (Oryza sativa L.). Plant Cell Physiol 38:1389–1396
• Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. California Agric Exp Stn Circ 347(1950):536–537
• Housley TL, Volenec JJ (1988) Fructan content and synthesis in leaf tissue of Festuca arundinacea. Plant Physiol 86:1247–1251
• Kühn C, Grof C (2010) Sucrose transporters of higher plants. Curr Opin Plant Biol 13:288–298
• Lalonde S, Wipf D, Frommer WB (2004) Transport mechanisms for organic forms of carbon and nitrogen between source and sink. Annu Rev Plant Biol 55:341–372
• Livaka KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25:402–408
• Meyer S, Lauterbach C, Niedermeier M, Barth I, Sjolund RD, Sauer N (2004) Wounding enhances expression of AtSUC3, a sucrose transporter from arabidopsis sieve elements and sink tissues. Plant Physiol 134:684–693
• Payyavula RS, Tay KHC, Tsai C-J, Harding SA (2011) The sucrose transporter family in Populus: the importance of a tonoplast PtaSUT4 to biomass and carbon partitioning. Plant J 65:757–770
• Reinders A, Sivitz AB, Hsi A, Grof CP, Perroux JM, Ward JM (2006) Sugarcane ShSUT1: analysis of sucrose transport activity and inhibition by sucralose. Plant Cell Environ 29:1871–1880
• Riesmeier JW, Willmitzer L, Frommer WB (1992) Isolation and characterization of a sucrose carrier cDNA from spinach by functional expression in yeast. EMBO J 11:4705–4713
• Riesmeier JW, Willmitzer L, Frommer WB (1994) Evidence for an essential role of the sucrose transporter in phloem loading and assimilate partitioning. EMBO J 13:1–7
• Sauer N (2007) Molecular physiology of higher plant sucrose transporters. FEBS Lett 581:2309–2317
• Scofield GN, Hirose T, Aoki N, Furbank RT (2007) Involvement of the sucrose transporter, OsSUT1, in the long-distance pathway for assimilate transport in rice. J Exp Bot 58:3155–3169
• Sivitz AB, Reinders A, Johnson ME, Krentz AD, Grof CP, Perroux JM, Ward JM (2007) Arabidopsis sucrose transporter AtSUC9. High-affinity transport activity, intragenic control of expression, and early flowering mutant phenotype. Plant Physiol 143: 188–198
• Slewinski TL, Braun DM (2010) Current perspectives on the regulation of whole-plant carbohydrate partitioning. Plant Sci 178:341–349
• Slewinski TL, Meeley R, Braun DM (2009) Sucrose transporter 1 functions in phloem loading in maize leaves. J Exp Bot 60: 881–892
• Stadler R, Truernit E, Gahrtz M, Sauer N (1999) The AtSUC1 sucrose carrier may represent the osmotic driving force for anther dehiscence and pollen tube growth in Arabidopsis. Plant J 19:269–278
• Su M, Li XF, Ma XY, Peng XJ, Zhao AG, Cheng LQ, Chen SY, Liu GS (2011) Cloning two P5CS genes from bioenergy sorghum and their expression profiles under abiotic stresses and MeJA treatment. Plant Sci 181:652–659
• Su M, Li XX, Li XF, Cheng LQ, Qi DM, Chen SY, Liu GS (2013) Molecular characterization and defoliation-induced expression of a sucrose transporter LcSUT1 gene in sheep grass (Leymus chinesis). Plant Mol Biol Rep 31:1184–1191
• Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
• Volenec JJ (1986) Nonstructural carbohydrates in stem base components of tall fescue during regrowth. Crop Sci 26:122–127
• Wang F, Liu CZ (2009) Development of an economic refining strategy of sweet sorghum in the Inner Mongolia region of China. Energy Fuels 23:4137–4142
• Wang XL, Liu D, Li ZQ (2012) Effects of the coordination mechanism between roots and leaves induced by root-breaking and exogenous cytokinin spraying on the grazing tolerance of ryegrass. J Plant Res 125:407–416
• Weise A, Barker L, Kühn C, Lalonde S, Buschmann H, Frommer WB et al (2000) A new subfamily of sucrose transporters, SUT4, with low affinity/high capacity localized in enucleate sieve elements of plants. Plant Cell 12:1345–1355
• Zhou Y, Qu H, Dibley KE, Offler CE, Patrick JW (2007) A suite of sucrose transporters expressed in coats of developing legume seeds includes novel pH-independent facilitators. Plant J 49:750–764

Identyfikatory

DOI

10.1007/s11738-014-1505-0